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Hybridization and polyploidization effects on LTR-retrotransposon activation in potato genome


Hybridization and polyploidization are major forces in plant evolution and potatoes are not an exception. It is proposed that the proliferation of Long Terminal Repeat-retrotransposons (LTR-RT) is related to genome reorganization caused by hybridization and/or polyploidization. The main purpose of the present work was to evaluate the effect of interspecific hybridization and polyploidization on the activation of LTR-RT. We evaluated the proliferation of putative active LTR-RT in a diploid hybrid between the cultivated potato Solanum tuberosum and the wild diploid potato species S. kurtzianum, allotetraploid lines derived from this interspecific hybrid and S. kurtzianum autotetraploid lines (ktz-autotetraploid) using the S-SAP (sequence-specific amplified polymorphism) technique and normalized copy number determination by qPCR. Twenty-nine LTR-RT copies were activated in the hybrid and present in the allotetraploid lines. Major LTR-RT activity was detected in Copia-27, Copia-12, Copia-14 and, Gypsy-22. According to our results, LTR-RT copies were activated principally in the hybrid, there was no activation in allotetraploid lines and only one copy was activated in the autotetraploid.

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This work was supported by the following institutions from Argentina, Agencia Nacional de Promoción Científica y Técnica (ANPCyT) PICT 1243 and Universidad Nacional de Cuyo, Mendoza, Argentina, project 06/A597. G.M, M.A and I.V.N. are Consejo Nacional de Investigaciones Científica y Técnicas (CONICET) fellows.

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Correspondence to Magdalena Gantuz or Ricardo Williams Masuelli.

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Gantuz, M., Morales, A., Bertoldi, M.V. et al. Hybridization and polyploidization effects on LTR-retrotransposon activation in potato genome. J Plant Res 135, 81–92 (2022).

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  • Allotetraploid
  • Autotetraploid
  • Hybridization
  • Long terminal repeat retrotransposons
  • Solanum kurtzianum
  • Solanum tuberosum