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Screening of mutants using chlorophyll fluorescence

Abstract

Chlorophyll fluorescence has been widely used for the estimation of photosynthesis or its regulatory mechanisms. Chlorophyll fluorescence measurements are the methods with non-destructive nature and do not require contact between plant materials and fluorometers. Furthermore, the measuring process is very rapid. These characteristics of chlorophyll fluorescence measurements make them a suitable tool to screen mutants of photosynthesis-related genes. Furthermore, it has been shown that genes with a wide range of functions can be also analyzed by chlorophyll fluorescence through metabolic interactions. In this short review, we would like to first introduce the basic principle of the chlorophyll fluorescence measurements, and then explore the advantages and limitation of various screening methods. The emphasis is on the possibility of chlorophyll fluorescence measurements to screen mutants defective in metabolisms other than photosynthesis.

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Acknowledgements

This work was supported by Grant-in-Aid for Scientific Research on Innovative Areas (No. 16H06552 and No. 16H06553 to KS).

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Correspondence to Kintake Sonoike.

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Ogawa, T., Sonoike, K. Screening of mutants using chlorophyll fluorescence. J Plant Res 134, 653–664 (2021). https://doi.org/10.1007/s10265-021-01276-6

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Keywords

  • Chlorophyll fluorescence
  • Imaging
  • Kautsky transient
  • Metabolic interaction
  • OJIP analysis
  • Screening