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Fungal colonization associated with phenological stages of a photosynthetic terrestrial temperate orchid from the Southern Iberian Peninsula

  • Regular Paper – Taxonomy/Phylogenetics/Evolutionary Biology
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Abstract

Fungal endophytes, both mycorrhizal and non-mycorrhizal, are involved in the development of the life cycle of orchids, providing potential beneficial relationships. Here, we assess the succession of changes in the diversity of fungal symbionts associated with a terrestrial temperate orchid species, Anacamptis morio subsp. champagneuxii, over three phenological stages: developed leaves but no stem elongation, flowering, and fruiting. Fungi endophyte associated with roots were obtained by culture in sterile conditions. A total of 18 morphotypes—one Mortierellomycota, two Basidiomycota and 15 Ascomycota—were differentiated, and were also characterized using PCR and DNA sequencing techniques. Only three of the 18 OTUs are shared among the three phenological stages examined: Westerdykella sp., a member of Ceratobasidiaceae, and Fusarium oxysporum, representing a relative abundance of between 28% (fruiting) to 41% (flowering). Our research confirmed that fungal symbionts varied among the different phenological stages examined, the peak of endophyte diversity appearing in the flowering stage. The availability of a diverse mycobiota seems to be important for the survival of orchid plants because it may cover particular physiological needs, and knowledge concerning this mycobiota is of special relevance in the establishment of reliable conservation programmes.

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Acknowledgements

The authors would like to express their gratitude to Mr. Tony Krupa for reviewing the English version of this paper. The authors also appreciate the constructive comments and suggestions provided by two anonymous reviewers and the editor of JPRE on an early version of the manuscript.

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  1. Irene Herrera-Rus and Rocío Juan contributed equally to this work.

Authors and Affiliations

  1. Departamento de Biología Vegetal y Ecología, Facultad de Farmacia, Universidad de Sevilla, Profesor García González, 2, 41012, Sevilla, Spain

    Irene Herrera-Rus & Rocío Juan

  2. Departamento de Biología Vegetal y Ecología, Facultad de Biología, Universidad de Sevilla, Reina Mercedes, 6, 41012, Sevilla, Spain

    Julio E. Pastor

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  1. Irene Herrera-Rus
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  2. Julio E. Pastor
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Correspondence to Rocío Juan.

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Herrera-Rus, I., Pastor, J.E. & Juan, R. Fungal colonization associated with phenological stages of a photosynthetic terrestrial temperate orchid from the Southern Iberian Peninsula. J Plant Res 133, 807–825 (2020). https://doi.org/10.1007/s10265-020-01225-9

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