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DORMANCY/AUXIN ASSOCIATED FAMILY PROTEIN 2 of Arabidopsis thaliana is a negative regulator of local and systemic acquired resistance

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Abstract

To fine tune defense response output, plants recruit both positive and negative regulators. Here we report Arabidopsis DORMANCY/AUXIN ASSOCIATED FAMILY PROTEIN 2(DAP2) gene as a negative regulator of basal defense against virulent bacterial pathogens. Expression of DAP2 enhances upon pathogen inoculation. Our experiments show that DAP2 suppressed resistance against virulent strains of bacterial pathogens, pathogen-induced callose deposition, and ROS accumulation; however, it did not influence effector-triggered immunity. In addition, DAP2 negatively regulated systemic acquired resistance (SAR). DAP2 expression was enhanced in the pathogen-free systemic tissues of SAR-induced plants. Previously, Arabidopsis Flowering locus D (FLD) gene has been shown to be essential for SAR but not for local resistance. We show here that FLD function is necessary for SAR-induced expression of DAP2, suggesting DAP2 as a target of FLD for activation of SAR in Arabidopsis.

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Acknowledgements

We acknowledge Prof. Ping He, Texas A&M University, USA for Pst hrcC strain, Arabidopsis Biological Resource Centre, Ohio State University, USA for the mutant seeds. This work was financially supported by DBT Grant (No. BT/PR23632/BPA/118/312/2017) to AKN. SR and SS acknowledge CSIR and JNU Non-net merit fellowship, respectively. The authors also acknowledge infrastructural supports received from DST-FIST and PURSE programs.

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  1. Shweta Roy and Shobhita Saxena have contributed equally.

Authors and Affiliations

  1. 415, School of Life Science, Jawaharlal Nehru University, New Delhi, 110067, India

    Shweta Roy, Shobhita Saxena, Aviroop Sinha & Ashis Kumar Nandi

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  1. Shweta Roy
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  2. Shobhita Saxena
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  3. Aviroop Sinha
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  4. Ashis Kumar Nandi
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AKN conceptualized and designed most of the experiments. SR, SS, and AS designed some of the experiments and performed all the experiments. SS and AKN wrote the manuscript, which has been further modified and approved by all authors.

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Correspondence to Ashis Kumar Nandi.

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Roy, S., Saxena, S., Sinha, A. et al. DORMANCY/AUXIN ASSOCIATED FAMILY PROTEIN 2 of Arabidopsis thaliana is a negative regulator of local and systemic acquired resistance. J Plant Res 133, 409–417 (2020). https://doi.org/10.1007/s10265-020-01183-2

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  • DOI: https://doi.org/10.1007/s10265-020-01183-2

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