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Journal of Plant Research

, Volume 132, Issue 4, pp 509–520 | Cite as

Spatiotemporal variation in phenolic levels in galls of calophyids on Schinus polygama (Anacardiaceae)

  • Lubia M. Guedes
  • Narciso Aguilera
  • Bruno G. Ferreira
  • Sebastián Riquelme
  • Katia Sáez-Carrillo
  • José Becerra
  • Claudia Pérez
  • Evelyn Bustos
  • Rosy M. S. IsaiasEmail author
Regular Paper

Abstract

The expression of plant secondary metabolism is strongly controlled by plant both in time and space. Although the variation of secondary metabolites, such as soluble and structural phenolics (e.g., lignins), has been largely observed in gall-inducing insects, and compared to their non-galled host organs, only a few datasets recording such variation are available. Accordingly, the relative importance of spatiotemporal variability in phenolic contents, and the influence of gall developmental stages on the original composition of host organs are poorly discussed. To address this knowledge gap, we histochemically determined the sites of polyphenol and lignin accumulation, and the polyphenol contents in three developmental stages of two calophyid galls and their correspondent host organs. Current results indicate that the compartmentalization of phenolics and lignins on Schinus polygama (Cav.) Cabrera follows a similar pattern in the two-calophyid galls, accumulating in the outer (the external tissue layers) and in the inner tissue compartments (the cell layers in contact with the gall chamber). The non-accumulation in the median compartment (median parenchyma layers of gall wall with vascular bundles, where gall inducer feeds) is important for the inducer, because its mouth apparatus enter in contact with the cells of this compartment. Also, the concentration of phenolics has opposite dynamics, decreasing in leaf galls and increasing in stem galls, in temporal scale, i.e., from maturation toward senescence. The concentration of phenolics in non-galled host organs, and in both galls indicated the extended phenotype of Calophya rubra (Blanchard) and C. mammifex Burckhardt & Basset (Hemiptera: Sternorrhyncha: Psylloidea: Calophyidae) over the same host plant metabolic potentiality.

Keywords

Calophyidae Compartmentalization Gall Lignins Polyphenols Schinus polygama 

Notes

Acknowledgements

This work was supported by the Comisión Nacional de Investigación Científica y Tecnológica (CONICYT) under Grant 63140050 (National PhD/2014-fellowship) awarded to LMG, Projects REDI170025 and MEC80170028 funded by CONICYT, Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). The authors are grateful to PD Dr. Daniel Burckhardt (Naturhistorisches Museum Switzerland) for his contribution on insect identification.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10265_2019_1118_MOESM1_ESM.pdf (112 kb)
Supplementary material 1 (PDF 111 kb)

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Copyright information

© The Botanical Society of Japan and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  • Lubia M. Guedes
    • 1
  • Narciso Aguilera
    • 1
  • Bruno G. Ferreira
    • 2
  • Sebastián Riquelme
    • 3
  • Katia Sáez-Carrillo
    • 4
  • José Becerra
    • 5
  • Claudia Pérez
    • 5
  • Evelyn Bustos
    • 5
  • Rosy M. S. Isaias
    • 6
    Email author
  1. 1.Departamento de Silvicultura, Facultad de Ciencias ForestalesUniversidad de ConcepciónConcepciónChile
  2. 2.Departamento de Botânica, Instituto de BiologiaUniversidade Federal do Rio de Janeiro (UFRJ)Rio de JaneiroBrazil
  3. 3.Unidad de Desarrollo Tecnológico (UDT)Universidad de ConcepciónCoronelChile
  4. 4.Departamento de Estadística, Facultad de Ciencias Físicas y MatemáticasUniversidad de ConcepciónConcepciónChile
  5. 5.Departamento de Botánica, Facultad de Ciencias Naturales y OceanográficasUniversidad de ConcepciónConcepciónChile
  6. 6.Departamento de Botânica, Instituto de Ciências BiológicasUniversidade Federal de Minas GeraisBelo HorizonteBrazil

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