Journal of Plant Research

, Volume 132, Issue 4, pp 473–480 | Cite as

Effects of light conditions on growth and defense compound contents of Datura inoxia and D. stramonium

  • Itsuka Hirano
  • Hitomi Iida
  • Yasuaki Ito
  • Ho-Dong Park
  • Koichi TakahashiEmail author
Regular Paper


We examined the effects of light conditions on plant growth and production of defense compounds in the toxic species Datura inoxia and D. stramonium. Specifically, we investigated morphological and physiological traits, including the contents of nitrogen-based tropane alkaloids (atropine and scopolamine) as defense compounds, under three light conditions: 100%, 80%, and 50% of full sunlight. Both species showed similar morphological and physiological responses to exposure to different intensities of light. Although the total plant mass decreased under lower light conditions, the total leaf area per plant increased. The reason being that the leaf mass per plant did not decrease, while the leaf mass per unit area decreased. Leaf nitrogen and chlorophyll concentrations and the chlorophyll/nitrogen ratio increased under lower light conditions, whereas the chlorophyll a/b ratio decreased. These morphological and physiological changes may be seen as ways to increase light acquisition under low light conditions. Leaf atropine and scopolamine concentrations did not differ among the three light conditions for both species. In conclusion, both Datura species underwent morphological and physiological changes under low light conditions, enabling them to use carbon and nitrogen to increase light acquisition while maintaining their chemical defense capability.


Carbon allocation Chlorophyll Leaf nitrogen Plasticity Tropane alkaloid 



This study was partially supported by grants from the Ministry of Education, Culture, Sports, Science and Technology, Japan.


  1. Aguilar-Chama A, Guevara R (2016) Resource allocation in an annual herb: effects of light, mycorrhizal fungi, and defoliation. Acta Oecol 71:1–7CrossRefGoogle Scholar
  2. Anten NPR, Miyazawa K, Hikosaka K, Nagashima H, Hirose T (1998) Leaf nitrogen distribution in relation to leaf age and photon flux density in dominant and subordinate plants in dense stands of a dieotyledonous herb. Oecologia 113:314–324CrossRefGoogle Scholar
  3. Brown PD, Tokuhisa JG, Reichelt M, Gershenzon J (2003) Variation of glucosinolate accumulation among different organs and developmental stages of Arabidopsis thaliana. Phytochemistry 62:471–481CrossRefGoogle Scholar
  4. Burns AE, Gleadow RM, Woodrow IE (2002) Light alters the allocation of nitrogen to cyanogenic glycosides in Eucalyptus cladocalyx. Oecologia 133:288–294CrossRefGoogle Scholar
  5. Cai ZQ, Chen YJ, Bongers F (2007) Seasonal changes in photosynthesis and growth of Zizyphus attopensis seedlings in three contrasting microhabitats in a tropical seasonal rain forest. Tree Physiol 27:827–836CrossRefGoogle Scholar
  6. Cai ZQ, Wang WH, Yang J, Cai CT (2009) Growth, photosynthesis and root reserpine concentrations of two Rauvolfia species in response to a light gradient. Ind Crop Prod 30:220–226CrossRefGoogle Scholar
  7. Castillo G, Cruz LL, Hernández-Cumplido J, Oyama K, Flores-Ortiz CM, Fornoni J, Valverde PL, Núñez-Farfán J (2013) Geographic association and temporal variation of chemical and physical defense and leaf damage in Datura stramonium. Ecol Res 28:663–672CrossRefGoogle Scholar
  8. Castillo G, Cruz LL, Tapia-Lopez R, Olmedo-Vicente E, Carmona D, Anaya-Lang AL, Fornoni J, Andraca-Gomez G, Valverde PL, Nunez-Farfan J (2014) Selection mosaic exerted by specialist and generalist herbivores on chemical and physical defense of Datura stramonium. PLoS One 9:e102478CrossRefGoogle Scholar
  9. Cipollini D, Walters D, Voelckel C (2014) Costs of resistance in plants: from theory to evidence. Annu Plant Rev 47:263–308CrossRefGoogle Scholar
  10. Cisneros-Silva A, Castillo G, Chávez-Pesqueira M, Bello-Bedoy R, Camargo ID, Núñez-Fárfan J (2017) Light limitation reduces tolerance to leaf damage in Datura stramonium. Evol Ecol Res 18:351–362Google Scholar
  11. Coley PD, Bryant JP, Chapin FS III (1985) Resource availability and plant antiherbivore defense. Science 230:895–899CrossRefGoogle Scholar
  12. Cronin G, Lodge DM (2003) Effects of light and nutrient availability on the growth, allocation, carbon/nitrogen balance, phenolic chemistry, and resistance to herbivory of two freshwater macrophytes. Oecologia 137:32–41CrossRefGoogle Scholar
  13. Dudt JF, Shure DJ (1994) The influence of light and nutrients on foliar phenolics and insect herbivory. Ecology 75:86–98CrossRefGoogle Scholar
  14. Elle E, Hare JD (2000) No benefit of glandular trichome production in natural populations of Datura wrightii? Oecologia 123:57–65CrossRefGoogle Scholar
  15. Elle E, van Dam NM, Hare JD (1999) Cost of glandular trichomes, a “resistance” character in Datura wrightii Regel (Solanaceae). Evolution 53:22–35Google Scholar
  16. Evans JR (1989) Photosynthesis and nitrogen relationships in leaves of C3 plants. Oecologia 78:9–19CrossRefGoogle Scholar
  17. Gianoli E, Molina-Montenegro MA, Becerra J (2007) Interactive effects of leaf damage, light intensity and support availability on chemical defenses and morphology of a twining vine. J Chem Ecol 33:95–103CrossRefGoogle Scholar
  18. Gratani L, Covone F, Larcher W (2006) Leaf plasticity in response to light of three evergreen species of the Mediterranean maquis. Trees 20:549–558CrossRefGoogle Scholar
  19. Griffin WJ, Lin GD (2000) Chemotaxonomy and geographical distribution of tropane alkaloids. Phytochemistry 53:623–637CrossRefGoogle Scholar
  20. Herms DA, Mattson WJ (1992) The dilemma of plants: to grow or defend. Q Rev Biol 67:283–335CrossRefGoogle Scholar
  21. Hikosaka K, Terashima I (1995) A model of the acclimation of photosynthesis in the leaves of C3 plants to sun and shade with respect to nitrogen use. Plant Cell Environ 18:605–618CrossRefGoogle Scholar
  22. Hikosaka K, Terashima I (1996) Nitrogen partitioning among photosynthetic components and its consequence in sun and shade plants. Funct Ecol 10:335–343CrossRefGoogle Scholar
  23. Hirata K, Horiuchi M, Asada M, Ando T, Miyamoto K, Miura Y (1992) Stimulation of dimeric alkaloid production by near-ultraviolet light in mutiple shoot cultures of Catharanthus roseus. J Ferment Bioeng 74:222–225CrossRefGoogle Scholar
  24. Hirose T, Werger MJA (1987) Maximizing daily canopy photosynthesis with respect to the leaf nitrogen allocation pattern in the canopy. Oecologia 72:520–526CrossRefGoogle Scholar
  25. Höft M, Verpoorte R, Beck E (1996) Growth and alkaloid contents in leaves of Tabernaemontana pachysiphon Stapf (Apocynaceae) as influenced by light intensity, water and nutrient supply. Oecologia 107:160–169CrossRefGoogle Scholar
  26. Husen AH, Ali ST, Mahmooduzafar Iqbal M (1999) Structural, functional and biochemical responses of Datura innoxia Mill. to coal-smoke pollution. Proc Acad Environ Biol 8:61–72Google Scholar
  27. Ibrahim HM, Abdo NA, Al Masaudi ES, Al-Gifri ANA (2016) Morphological, epidermal and anatomical properties of Datura linn. Leaf in Sana’a City–Yemen and its taxonomical significance. Asian J Plant Sci Res 6:69–80Google Scholar
  28. Kang JH, Shi F, Jones AD, Marks MD, Howe GA (2010) Distortion of trichome morphology by the hairless mutation of tomato affects leaf surface chemistry. J Exp Bot 61:1053–1064CrossRefGoogle Scholar
  29. Kariñho-Betancourt E, Agrawal AA, Halitschke R, Núñez-Farfán J (2015) Phylogenetic correlations among chemical and physical plant defenses change with ontogeny. New Phytol 206:796–806CrossRefGoogle Scholar
  30. Leete E (1979) Biosynthesis and metabolism of the tropane alkaloids. J Med Plant Res 36:97–112CrossRefGoogle Scholar
  31. Lind EM, Borer E, Seabloom E, Adler P, Bakker JD, Blumenthal DM, Crawley M, Davies K, Firn J, Gruner DS, Harpole WS, Hautier Y, Hillebrand H, Knops J, Melbourne B, Mortensen B, Risch AC, Schuetz M, Stevens C, Wragg PD (2013) Life-history constraints in grassland plant species: a growth-defence trade-off is the norm. Ecol Let 16:513–521CrossRefGoogle Scholar
  32. Lindsey K, Yeoman MM (1983) The relationship between growth rate, differentiation and alkaloid accumulation in cell cultures. J Exp Bot 43:1055–1065CrossRefGoogle Scholar
  33. Loyola-Vargas V, Méndez-Zeel M, Monforte-González M, Miranda-Ham ML (1992) Serpentine accumulation during greening in normal and tumor tissues of Catharanthus roseus. J Plant Physiol 140:213–217CrossRefGoogle Scholar
  34. McCall AC, Fordyce JA (2010) Can optimal defence theory be used to predict the distribution of plant chemical defences? J Ecol 98:985–992CrossRefGoogle Scholar
  35. McKey D (1974) Adaptive patterns in alkaloid physiology. Am Nat 108:305–320CrossRefGoogle Scholar
  36. Naidu SL, DeLucia EH (1997) Growth, allocation and water relations of shade-grown Quercus rubra L. saplings exposed to a late-season canopy gap. Ann Bot 80:335–344CrossRefGoogle Scholar
  37. Niinemets Ü (1997) Role of foliar nitrogen in light harvesting and shade tolerance of four temperate deciduous woody species. Funct Ecol 11:518–531CrossRefGoogle Scholar
  38. Peng G, Wu C, Xu X, Yang D (2012) The age-related changes of leaf structure and biochemistry in juvenile and mature subalpine fir trees (Abies faxoniana Rehder & E.H. Wilson.) along an altitudinal gradient. Polish J Ecol 60:311–321Google Scholar
  39. Poorter L (1999) Growth responses of 15 rain-forest tree species to a light gradient: the relative importance of morphological and physiological traits. Funct Ecol 13:396–410CrossRefGoogle Scholar
  40. Poorter L, Rozendaal DMA (2008) Leaf size and leaf display of thirty-eight tropical tree species. Oecologia 158:35–46CrossRefGoogle Scholar
  41. Porra RJ, Thompson WA, Kriedemann PE (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim Biophys Acta 975:384–394CrossRefGoogle Scholar
  42. R Core Team (2017) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Accessed 27 April 2018
  43. Rodriguez-Calcerrada J, Pardos JA, Gil L, Reich PB, Aranda I (2008) Light response in seedlings of temperate (Quercus petraea) and a sub-Mediterranean species (Quercus pyrenaica): contrasting ecological strategies as potential keys to regeneration performance in mixed marginal populations. Plant Ecol 195:273–285CrossRefGoogle Scholar
  44. Sangekar SN, Devarkar VD (2017) Pharmacognostical studies in Datura inoxia Mill. Biosci Discov 8:468–473Google Scholar
  45. Shimizu T (1997) Flora of Nagano prefecture. Shinano Mainichi Shinbunsha, Nagano (in Japanese) Google Scholar
  46. Shimizu N, Morita H, Hirota S (2001) Plant invader 600 species. Zenkoku Noson Kyouiku Kyokai, Tokyo (in Japanese) Google Scholar
  47. Strauss SY, Rudgers JA, Lau JA, Irwin R (2002) Direct and ecological costs of resistance to herbivory. Trend Ecol Evol 17:278–285CrossRefGoogle Scholar
  48. Takahashi K, Goto A (2012) Morphological and physiological responses of beech and oak seedlings to canopy conditions: why does beech dominate the understory of unmanaged oak fuelwood stands? Can J For Res 42:1623–1630CrossRefGoogle Scholar
  49. Takahashi K, Mikami Y (2006) Effects of canopy cover and seasonal reduction in rainfall on leaf phenology and leaf traits of the fern Oleandra pistillaris in a tropical montane forest, Indonesia. J Trop Ecol 22:599–604CrossRefGoogle Scholar
  50. Takahashi K, Rustandi A (2006) Responses of crown development to canopy openings by saplings of eight tropical submontane forest tree species in Indonesia: a comparison with cool temperate trees. Ann Bot 97:559–569CrossRefGoogle Scholar
  51. Takahashi K, Seino T, Kohyama T (2001) Responses to canopy openings in architectural development of saplings in eight deciduous broad-leaved tree species. Can J For Res 31:1336–1347CrossRefGoogle Scholar
  52. Takahashi K, Seino T, Kohyama T (2005) Plastic changes of leaf mass per area and leaf nitrogen content in response to canopy openings in saplings of eight deciduous broad-leaved tree species. Ecol Res 20:17–23CrossRefGoogle Scholar
  53. Valverde PL, Fornoni J, Nunez-Farfan J (2001) Defensive role of leaf trichomes in resistance to herbivorous insects in Datura stramonium. J Evol Biol 14:424–432CrossRefGoogle Scholar
  54. Van Hees AFM, Clerkx APPM (2003) Shading and root-shoot relations in saplings of silver birch, pedunculate oak and beech. For Ecol Manag 176:439–448CrossRefGoogle Scholar
  55. Van Horne B, Hanley TA, Cates RG, McKendrick JD, Horner JD (1988) Influence of seral stage and season on leaf chemistry of southeastern Alaska deer forage. Can J For Res 18:90–99CrossRefGoogle Scholar
  56. Welander NT, Ottosson B (1998) The influence of shading on growth and morphology in seedlings of Quercus robur L. and Fagus sylvatica L. For Ecol Manag 107:117–126CrossRefGoogle Scholar
  57. Zheng YL, Feng YL, Liu WX, Liao ZY (2009) Growth, biomass allocation, morphology, and photosynthesis of invasive Eupatorium adenophorum and its native congeners grown at four irradiances. Plant Ecol 203:263–271CrossRefGoogle Scholar

Copyright information

© The Botanical Society of Japan and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  • Itsuka Hirano
    • 1
  • Hitomi Iida
    • 1
  • Yasuaki Ito
    • 1
  • Ho-Dong Park
    • 2
  • Koichi Takahashi
    • 1
    • 3
    Email author
  1. 1.Department of Biology, Faculty of ScienceShinshu UniversityMatsumotoJapan
  2. 2.Department of Environmental Sciences, Faculty of ScienceShinshu UniversityMatsumotoJapan
  3. 3.Institute of Mountain ScienceShinshu UniversityMatsumotoJapan

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