Enhanced transcriptome responses in herbivore-infested tea plants by the green leaf volatile (Z)-3-hexenol
Green leaf volatiles (GLVs) play a vital role in enhancing herbivore-associated defense responses, but the mechanism by which they precisely regulate such responses is not well understood. (Z)-3-Hexenol (z3HOL), an important component of GLVs, effectively activates the defense of tea plants (Camellia sinensis) against a tea geometrid (TG) Ectropis obliqua Prout. To elucidate the molecular mechanisms of defense activation by z3HOL, RNA-Sequencing was employed to investigate the effect of z3HOL on transcriptome responses to TG in tea plants. A total of 318 upregulated genes were identified, and expression of 10 unigenes was validated by quantitative real-time PCR. Among these 318 upregulated genes, 56 were defense-related, including 6 key enzyme genes in jasmonic acid, and ethylene biosynthesis, 24 signal transduction genes, and 12 insect-responsive transcription factors. Most of the defense-related genes are induced by JA, TG, or wounding treatments, suggesting that JA signaling plays a vital role in z3HOL-induced tea defense against TG.
KeywordsTea (Z)-3-Hexenol Jasmonic acid RNA-Seq Ectropis obliqua Defense-related genes
Differentially expressed genes
Digital gene expression
Gas chromatography-mass spectrometry
Green leaf volatiles
Herbivore-induced plant volatiles
Mitogen-activated protein kinases
Quantitative real-time PCR
Relative air humidity
Receptor-like protein kinases
Xylanase inhibitor proteins
The study was jointly sponsored by the Special Fund for Agro-scientific Research in the Public Interest (201403030), the Central Public-interest Scientific Institution Basal Research Fund (no. 1610212016019), the National Natural Science Foundation of China (31401758), and the Science and Technology Program of Zhejiang Province (2016C32026).
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