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Journal of Plant Research

, Volume 131, Issue 5, pp 827–837 | Cite as

A DREPP protein interacted with PeaT1 from Alternaria tenuissima and is involved in elicitor-induced disease resistance in Nicotiana plants

  • Fanlu Meng
  • Yao Xiao
  • Lihua Guo
  • Hongmei Zeng
  • Xiufen Yang
  • Dewen Qiu
Regular Paper

Abstract

PeaT1 is a proteinaceous elicitor from fungal pathogen Alternaria tenuissima. Our previous research revealed that this elicitor could induce defense response and enhance disease resistance in various plants including Nicotiana plants. However, immune activation mechanisms whereby PeaT1 elicits defense response remain unclear. In this study, the association between elicitor protein PeaT1 and the plasma membrane was assessed using the FITC (Fluorescein isothiocyanate) labeling method. A PeaT1-interacting protein was isolated via 125I-PeaT1 cross-linking and Far Western blot analyses, and designated PtBP1 (PeaT1 Binding Protein 1). From the data of Mass spectrometry (MS) and bioinformatics analysis, the 22 kDa plasma membrane protein PtBP1 was inferred to be a member of DREPP (developmentally regulated plasma membrane polypeptide) family that is induced in plants under stress conditions and might get involved in downstream signaling. For further verification of this association, Far Western blot, co-immunoprecipitation and bimolecular fluorescence complementation (BiFC) analyses were performed, showing PtBP1 could bind with PeaT1 in vitro and in vivo. Virus-induced gene silencing (VIGS) analysis exhibited that PtBP1 silencing in Nicotiana benthamiana attenuated tobacco mosaic virus (TMV) resistance compared to the tobacco rattle virus (TRV) control after PeaT1 treatment.

Keywords

Disease resistance DREPP family Elicitor PeaT1 Protein–protein interaction 

Notes

Acknowledgements

This research was supported by the National Natural Science Foundation of China (Grant No. 31672097).

Supplementary material

10265_2018_1038_MOESM1_ESM.pdf (1.2 mb)
Supplementary material 1 (PDF 1191 KB)

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Copyright information

© The Botanical Society of Japan and Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  1. 1.The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant ProtectionChinese Academy of Agricultural ScienceBeijingChina

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