Journal of Plant Research

, Volume 131, Issue 1, pp 23–29 | Cite as

Reconstitutive approach for investigating plant vascular development

JPR Symposium Semi-in-vivo Developmental Biology

Abstract

Plants generate various tissues and organs via a strictly regulated developmental program. The plant vasculature is a complex tissue system consisting of xylem and phloem tissues with a layer of cambial cells in between. Multiple regulatory steps are involved in vascular development. Although molecular and genetic studies have uncovered a variety of key factors controlling vascular development, studies of the actual functions of these factors have been limited due to the inaccessibility of the plant vasculature. Thus, to obtain a different perspective, culture systems have been widely used to analyze the sequential processes that occur during vascular development. A tissue culture system known as VISUAL, in which molecular genetic analysis can easily be performed, was recently established in Arabidopsis thaliana. This reconstitutive approach to vascular development enables this process to be investigated quickly and easily. In this review, I summarize our recent knowledge of the regulatory mechanisms underlying vascular development and provide future perspectives on vascular analyses that can be performed using VISUAL.

Keywords

Vascular development In vitro culture system Signaling network Plant hormones Reconstitution 

Notes

Acknowledgements

This work was supported by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (17H06476 to YK), and from the Japan Society for the Promotion of Science (17H05008 to YK).

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Copyright information

© The Botanical Society of Japan and Springer Japan KK, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Department of Biological Sciences, Graduate School of ScienceThe University of TokyoBunkyo-kuJapan

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