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The responses of photosystem II and intracellular ATP production of Arabidopsis leaves to salt stress are affected by extracellular ATP

Abstract

Hypertonic salt stress with different concentrations of NaCl increased the levels of extracellular ATP of Arabidopsis leaves. And, hypertonic salt stress decreased the levels of F v /F m (the maximal efficiency of photosystem II), Φ PSII (the photosystem II operating efficiency), qP (photochemical quenching), and intracellular ATP (iATP) production. The treatment with β,γ-methyleneadenosine 5′-triphosphate (AMP-PCP), which can exclude extracellular ATP from its binding sites of extracellular ATP receptors, caused a further decrease in the levels of F v /F m , Φ PSII, qP, and iATP production of the salt-stressed Arabidopsis leaves, while the addition of exogenous ATP rescued the inhibitory effects of AMP-PCP on Φ PSII , qP, and iATP production under hypertonic salt stress. Under hypertonic salt stress, the values of F v /F m , Φ PSII , qP, and iATP production were lower in the dorn 13 mutant than in the wild-type plants. These results indicate that the responses of photosystem II and intracellular ATP production to salt stress could be affected by extracellular ATP.

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Acknowledgements

The authors thank the National Natural Science Foundation of China (Projects Nos. 31360044, 31560070 and 31260059) Key Project of Science and Technology Research of Chinese Ministry of Education (No. 211190), the Fundamental Research Funds for the Gansu Universities of Gansu Provincial Department of Finance, the University Scientific Research Project of Gansu Province (No. 2015A-007), and Youth Teacher Scientific Research Ability Promotion Plan Innovation Team Project of Northwest Normal University.

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Correspondence to Han-Qing Feng.

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Hou, QZ., Sun, K., Zhang, H. et al. The responses of photosystem II and intracellular ATP production of Arabidopsis leaves to salt stress are affected by extracellular ATP. J Plant Res 131, 331–339 (2018). https://doi.org/10.1007/s10265-017-0990-9

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  • DOI: https://doi.org/10.1007/s10265-017-0990-9

Keywords

  • Extracellular ATP
  • Hypertonic salt stress
  • Intracellular ATP
  • Photosystem II