Journal of Plant Research

, Volume 131, Issue 2, pp 319–329 | Cite as

Molecular characterization of cytosolic cysteine synthase in Mimosa pudica

  • Md. Harun-Ur- Rashid
  • Hironori Iwasaki
  • Shigeki Oogai
  • Masakazu Fukuta
  • Shahanaz Parveen
  • Md. Amzad Hossain
  • Toyoaki Anai
  • Hirosuke Oku
Regular Paper


In the cysteine and mimosine biosynthesis process, O-acetyl-l-serine (OAS) is the common substrate. In the presence of O-acetylserine (thiol) lyase (OASTL, cysteine synthase) the reaction of OAS with sulfide produces cysteine, while with 3-hydroxy-4-pyridone (3H4P) produces mimosine. The enzyme OASTL can either catalyze Cys synthesis or both Cys and mimosine. A cDNA for cytosolic OASTL was cloned from M. pudica for the first time containing 1,410 bp nucleotides. The purified protein product from overexpressed bacterial cells produced Cys only, but not mimosine, indicating it is Cys specific. Kinetic studies revealed that pH and temperature optima for Cys production were 6.5 and 50 °C, respectively. The measured Km, Kcat, and Kcat Km−1 values were 159 ± 21 µM, 33.56 s−1, and 211.07 mM−1s−1 for OAS and 252 ± 25 µM, 32.99 s−1, and 130.91 mM−1s−1 for Na2S according to the in vitro Cys assay. The Cy-OASTL of Mimosa pudica is specific to Cys production, although it contains sensory roles in sulfur assimilation and the reduction network in the intracellular environment of M. pudica.


α-Aminoacrylate Cysteine 2,5-Dimethyl-3-pyridinol O-acetylserine (thiol) lyase Mimosine 



We are grateful to Dr. Shinichi Gima of the Instrumental Research Center, University of the Ryukyus, and Dr. Michael Chandro Roy of the Okinawa Institute of Science and Technology for providing technical assistance during the LC–MS/MS analyses. We sincerely thank to Dr. Rafiq Islam (Professor, Department of Natural Sciences, Northwest Missouri State University) for useful discussion and necessary corrections of our manuscript.

Supplementary material

10265_2017_986_MOESM1_ESM.docx (615 kb)
Supplementary material 1 (DOCX 615 KB)


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Copyright information

© The Botanical Society of Japan and Springer Japan KK 2017

Authors and Affiliations

  • Md. Harun-Ur- Rashid
    • 1
    • 5
  • Hironori Iwasaki
    • 3
  • Shigeki Oogai
    • 1
  • Masakazu Fukuta
    • 2
  • Shahanaz Parveen
    • 1
    • 5
  • Md. Amzad Hossain
    • 2
  • Toyoaki Anai
    • 4
  • Hirosuke Oku
    • 3
  1. 1.The United Graduate School of Agricultural SciencesKagoshima UniversityKagoshimaJapan
  2. 2.Graduate School of AgricultureUniversity of the RyukyusOkinawaJapan
  3. 3.Tropical Biosphere Research CenterUniversity of the RyukyusOkinawaJapan
  4. 4.Faculty of AgricultureSaga UniversitySagaJapan
  5. 5.Faculty of AgricultureSher-e-Bangla Agricultural UniversityDhakaBangladesh

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