The mechanism of monolignol transportation from the cytosol to the apoplast is still unclear despite being an essential step of lignification. Recently, ATP-binding cassette (ABC) transporters were suggested to be involved in monolignol transport. However, there are no reliable clues to the transporters of the major lignin monomers coniferyl and synapyl alcohol. In this study, the lignification progress of Arabidopsis cultured cells during tracheary element differentiation was monitored. The expression of selected transporter genes, as well as lignification and cell-wall formation related genes as references, in differentiating cultured cell samples harvested at 2-day intervals was analyzed by real-time PCR and the data were statistically processed. The cell wall formation transcription factor MYB46, programmed-cell death related gene XCP1 and lignin polymerization peroxidase AtPrx25 were classified into the same cluster. Furthermore, the cluster closest to the abovementioned cluster contained the lignin synthesis transcription factor MYB58 and the Arabidopsis ABC transporters ABCG11, ABCG22, ABCG36 and ABCG29. This result suggested that these four ABC transporters may be involved in lignification. In the expression analysis, unexpectedly, the lignification-related genes CAD5 and C4H were not included in the same cluster as MYB58 and AtPrx25. The expression data also suggested that the lignification of tracheary elements in the culture, where lignification ratio finally reached to around 40%, continued after cell death because lignification actively progressed after programmed cell death-related gene started to be expressed.
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This work was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI Scientific Research (B) Grant number JP26292097 (Y.T.), and JSPS KAKENHI Exploratory Research Grant number JP15K14774 (Y.T.).
Axelos M, Curie C, Mazzolini L et al (1992) A protocol for transient gene expression in Arabidopsis thaliana protoplasts isolated from cell suspension cultures. Plant Physiol Biochem 30:123–128Google Scholar
BellLelong DA, Cusumano JC, Meyer K, Chapple C (1997) Cinnamate-4-hydroxylase expression in Arabidopsis—regulation in response to development and the environment. Plant Phys 113:729–738. doi:10.1104/pp.113.3.729CrossRefGoogle Scholar
Campe R, Langenbach C, Leissing F et al (2016) ABC transporter PEN3/PDR8/ABCG36 interacts with calmodulin that, like PEN3, is required for Arabidopsis nonhost resistance. New Phytol 209:294–306. doi:10.1111/nph.13582CrossRefPubMedGoogle Scholar
Dittgen J, Sa C, Hou B et al (2006) Plant fungal infection process Arabidopsis PEN3/PDR8, an ATP binding cassette transporter, contributes to nonhost resistance to inappropriate pathogens that enter by direct penetration. Society 18:731–746. doi:10.1105/tpc.105.038372.1Google Scholar
Ehlting J, Mattheus N, Aeschliman DS et al (2005) Global transcript profiling of primary stems from Arabidopsis thaliana identifies candidate genes for missing links in lignin biosynthesis and transcriptional regulators of fiber differentiation. Plant J 42:618–640. doi:10.1111/j.1365-313X.2005.02403.xCrossRefPubMedGoogle Scholar
Fukuda H, Komamine A (1982) Lignin synthesis and its related enzymes as markers of tracheary-element differentiation in single cells isolated from the mesophyll of Zinnia elegans. Planta 155:423–430CrossRefPubMedGoogle Scholar
Hosokawa M (2001) Progress of lignification mediated by intercellular transportation of monolignols during tracheary element differentiation of isolated Zinnia mesophyll cells. Plant Cell Physiol 42:959–968. doi:10.1093/pcp/pce124CrossRefPubMedGoogle Scholar
Lu X, Dittgen J, Piślewska-Bednarek M et al (2015) Mutant allele-specific uncoupling of PENETRATION3 functions reveals engagement of the ATP-binding cassette transporter in distinct tryptophan metabolic pathways. Plant Physiol 168:814–827. doi:10.1104/pp.15.00182CrossRefPubMedPubMedCentralGoogle Scholar
Nilsson R, Bernfur K, Gustavsson N et al (2010) Proteomics of plasma membranes from poplar trees reveals tissue distribution of transporters, receptors, and proteins in cell wall formation. Mol Cell Proteomics 9:368–387. doi:10.1074/mcp.M900289-MCP200CrossRefPubMedGoogle Scholar
Panikashvili D, Shi JX, Bocobza S et al (2010) The arabidopsis DSO/ABCG11 transporter affects cutin metabolism in reproductive organs and suberin in roots. Mol Plant 3:563–575. doi:10.1093/mp/ssp103CrossRefPubMedGoogle Scholar
Shigeto J, Kiyonaga Y, Fujita K et al (2013) Putative cationic cell-wall-bound peroxidase homologues in arabidopsis, AtPrx2, AtPrx25, and AtPrx71, are involved in lignification. J Agric Food Chem 61:3781–3788. doi:10.1021/jf400426gCrossRefPubMedGoogle Scholar
Shigeto J, Itoh Y, Hirao S et al (2015) Simultaneously disrupting AtPrx2, AtPrx25 and AtPrx71 alters lignin content and structure in Arabidopsis stem. J Integr Plant Biol 57:349–356. doi:10.1111/jipb.12334CrossRefPubMedGoogle Scholar
Tokunaga N, Sakakibara N, Umezawa T et al (2005) Involvement of extracellular dilignols in lignification during tracheary element differentiation of isolated Zinnia mesophyll cells. Plant Cell Physiol 46:224–232. doi:10.1093/pcp/pci017CrossRefPubMedGoogle Scholar
Zhong R, Richardson EA, Ye Z (2007) The MYB46 transcription factor is a direct target of SND1 and regulates secondary wall biosynthesis in Arabidopsis The MYB46 transcription factor is a direct target of SND1 and regulates secondary wall biosynthesis in Arabidopsis. Plant Cell 19:2776–2792. doi:10.1105/tpc.107.053678CrossRefPubMedPubMedCentralGoogle Scholar