Journal of Plant Research

, Volume 131, Issue 1, pp 91–97 | Cite as

Genetic analysis of Japanese and American specimens of Scirpus hattorianus suggests its introduction from North America

  • Kohei Satoh
  • Kohtaroh Shutoh
  • Takahide Kurosawa
  • Eisuke Hayasaka
  • Shingo Kaneko
Regular Paper


Scirpus hattorianus is a possible alien species in Japan, and a clarification of its unclear taxonomy is required to reveal its origin. It is not known whether the plants initially described from Japan represent the same species distributed in North America. To clarify the origin of the species, we attempted to sequence old specimens collected about 80 years ago using newly designed primer pairs specific for short sequences, including the variable sites. Chloroplast sequences of ndhF were compared among Japanese and North American S. hattorianus, and the closely related species, S. atrovirens, S. flaccidifolius, and S. georgianus. We succeeded in sequencing all samples, and two haplotypes were detected in S. hattorianus: one was unique to the species and the other, detected from specimens potentially collected from the same population as the types, was shared by both North American S. hattorianus and two closely related species, S. atrovirens and S. flaccidifolius. Our results suggest that Japanese S. hattorianus is an alien species that was introduced from North America at least twice.


Alien species Chloroplast DNA Cyperaceae Herbarium specimens Scirpus hattorianus Taxonomy 



We thank Drs. David E. Boufford, A. R. Brach (Harvard University), M. Maki, K. Yonekura (Tohoku University), H. Nagamasu (Kyoto University), and H. Ikeda (Tokyo University), and Ms. A. Shimizu (Tokyo University), for their cooperation in the collection of specimen samples, and Messrs. K. Fukatsu (Notsuke Peninsule Nature Center) and S. Nemoto (Fukushima University), and Dr. Y. Kato (Kushiro City Museum), for their help in collecting samples. This work was supported by the Research Project of Fukushima University for Regeneration of Harmonies between Human Activity and Nature in Bandai-Asahi National Park.

Supplementary material

10265_2017_976_MOESM1_ESM.docx (21 kb)
Supplementary material 1 (DOCX 20 KB)


  1. Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797. doi: 10.1093/nar/gkh340 CrossRefPubMedPubMedCentralGoogle Scholar
  2. Govaerts R, Simpson DA, Goetghebeur P, Wilson KL, Egorova T, Bruhl J (2007) World checklist of Cyperaceae. The Board of Trustees of the Royal Botanic Gardens, Kew, RichmondGoogle Scholar
  3. Ishida Y, Demeke Y, de Groot PJVC, Georgiadis NJ, Leggett KE, Fox VE, Roca AL (2012) Short amplicon microsatellite markers for low quality elephant DNA. Cons Genet Res 4:491–494. doi: 10.1007/s12686-011-9582-5 CrossRefGoogle Scholar
  4. Jung J, Choi HK (2010) Systematic rearrangement of Korean Scirpus L. sl (Cyperaceae) as inferred from nuclear ITS and chloroplast rbcL sequences. J Plant Biol 53:222–232. doi: 10.1007/s12374-010-9109-8 CrossRefGoogle Scholar
  5. Kato Y, Fukatsu K (2016) A new record of Scirpus hattorianus Makino (Cyperaceae) from Hokkaido. J Jpn Bot 91:41–46 (Japanese)Google Scholar
  6. Kurosawa T, Hasunuma K, Kaneko S, Tanaka N, Hayasaka E (2015) Distribution of Scirpus hattorianus and S. georgianus (Cyperaceae) in Japan and the former’s putative North-American origin. Bunrui 15:29–40Google Scholar
  7. Léveillé-Bourret É, Gilmour CN, Starr JR, Naczi RF, Spalink D, Sytsma KJ (2014) Searching for the sister to sedges (Carex): resolving relationships in the Cariceae-Dulichieae-Scirpeae clade (Cyperaceae). Bot J Linn Soc 176:1–21. doi: 10.1111/boj.12193 CrossRefGoogle Scholar
  8. Makino T (1933) A contribution to the knowledge of the flora of Nippon. J Jpn Bot 8:44–45Google Scholar
  9. Muasya AM, Simpson DA, Verboom GA, Goetghebeur P, Naczi RF, Chase MW, Smets E (2009) Phylogeny of Cyperaceae based on DNA sequence data: current progress and future prospects. Bot Rev 75:2–21. doi: 10.1007/s12229-008-9019-3 CrossRefGoogle Scholar
  10. Murata G (1975) Discovery of Scirpus hattorianus in Shiga Prefecture. Acta Phytotax Geobot 27:28 (Japanese)Google Scholar
  11. Ohwi J (1943) Cyperaceae Japonicae, II: A synopsis of the Rhynchosporoideae and Scirpoideae of Japan, including the Kuriles, Saghalin, Korea, and Formosa. Mem Coll Sci, Kyoto Imp Univ. Ser B Biol 18:1–182Google Scholar
  12. Ohwi J (1982) Cyperaceae. In: Satake Y, Ohwi J, Kitamura S, Watari S, Tominari T (eds) Wild Flowers of Japan. Herbaceous plants 1. Heibonsha, Tokyo, pp 145–184 (Japanese)Google Scholar
  13. Pääbo S, Poinar H, Serre D, Jaenicke-Després V, Hebler J, Rohland N, Kuch M, Krause J, Vigilant L, Hofreiter M (2004) Genetic analyses from ancient DNA. Annu Rev Genet 38:645–679. doi: 10.1146/annurev.genet.37.110801.143214 CrossRefPubMedGoogle Scholar
  14. Saltonstall K (2002) Cryptic invasion by a non-native genotype of the common reed, Phragmites australis, into North America. Proc Natl Acad Sci 99:2445–2449. doi: 10.1073/pnas.032477999 CrossRefPubMedPubMedCentralGoogle Scholar
  15. Savolainen V, Cuénoud P, Spichiger R, Martinez MD, Crèvecoeur M, Manen JF (1995) The use of herbarium specimens in DNA phylogenetics: evaluation and improvement. Plant Syst Evol 197:87–98. doi: 10.1007/BF00984634 CrossRefGoogle Scholar
  16. Schuyler AE (1967a) Scirpus hattorianus in North America. Not Nat 398:1–5Google Scholar
  17. Schuyler AE (1967b) A taxonomic revision of North-American leafy species of Scirpus. Proc Acad Nat Sci Philadelphia 119:295–323Google Scholar
  18. Strong MT (1994) Taxonomy of Scirpus. Trichophorum, and Schoenoplectus (Cyperaceae) in Virginia.Bartonia 58:29–68Google Scholar
  19. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729. doi: 10.1093/molbev/mst197 CrossRefPubMedPubMedCentralGoogle Scholar
  20. Untergasser A, Cutcutache I, Koressaar T, Ye J, Faircloth BC, Remm M, Rozen SG (2012) Primer3—new capabilities and interfaces. Nucleic Acids Res 40:e115–e115. doi: 10.1093/nar/gks596 CrossRefPubMedPubMedCentralGoogle Scholar
  21. Valentini A, Miquel C, Nawaz MA, Bellemain EVA, Coissac E, Pompanon F, Gielly L, Cruaud C, Nascetti G, Wincker P, Swenson JE (2009) New perspectives in diet analysis based on DNA barcoding and parallel pyrosequencing: the trnL approach. Mol Ecol Res 9:51–60. doi: 10.1111/j.1755-0998.2008.02352.x CrossRefGoogle Scholar
  22. Verloove F (2014) Scirpus hattorianus (Cyperaceae), newly reported for Europe, naturalized in France. Willdenowia 44:51–55. doi: 10.3372/wi.44.44108 CrossRefGoogle Scholar
  23. Whittemore AT, Schuyler AE (2002) Scirpus. In: Flora of North America Editorial Committee (ed) Flora of North America vol 23. Oxford University Press, Oxford, pp 8–21Google Scholar

Copyright information

© The Botanical Society of Japan and Springer Japan KK 2017

Authors and Affiliations

  • Kohei Satoh
    • 1
  • Kohtaroh Shutoh
    • 1
    • 2
  • Takahide Kurosawa
    • 1
  • Eisuke Hayasaka
    • 3
  • Shingo Kaneko
    • 1
  1. 1.Faculty of Symbiotic Systems ScienceFukushima UniversityFukushimaJapan
  2. 2.Faculty of EducationNiigata UniversityNiigataJapan
  3. 3.Fukui Botanical GardenFukuiJapan

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