Journal of Plant Research

, Volume 131, Issue 1, pp 125–141 | Cite as

Diversity and evolution of leaf anatomical characters in Taxaceae s.l.—fluorescence microscopy reveals new delimitating characters

  • Christoph Elpe
  • Patrick Knopf
  • Thomas Stützel
  • Christian Schulz
Regular Paper
  • 279 Downloads

Abstract

Taxaceae s.l. comprise six genera (including Cephalotaxus) and about 35 species; The present study aims to give new insights into the evolution of this family, especially into the phylogenetic position of Cephalotaxus. Moreover, only little is known about comparative leaf anatomy of this family and this study aims to expose and interpret the diversity and evolution of leaf anatomical characters and to assess their applicability to identify taxa at the generic and species level. A detailed phylogeny was reconstructed, using both maximum likelihood and Bayesian inference, with a combined dataset of four molecular markers from the plastid and nuclear genomes. Leaf sections from 132 specimens, representing 32 species and four varieties (fresh and herbarium material) were inspected, using fluorescence microscopy. Ancestral characters were reconstructed using Mesquite. The phylogenetic analyses provided full support for Cephalotaxus as sister group to Taxaceae s.str. Within the latter, two monophyletic tribes Taxeae (comprising Austrotaxus, Pseudotaxus, and Taxus) and Torreyeae (comprising Amentotaxus and Torreya) were fully supported. Fluorescence microscopy was shown to be very useful for identifying leaf tissues and their constitution. We were able to show that particularly sclerified tissues have highest potential for the discrimination of both freshly collected samples and rehydrated herbarium specimens at the generic and species level. A correlation between the presence of different sclereid types could be shown and sclereids were hypothesized to pose a primitive trait in the evolution of Taxaceae s.l. New identification keys were generated on the basis of leaf anatomical characters. The microscopic method presented here is applicable for further studies within gymnosperms and probably in angiosperms, as well.

Keywords

Character evolution Conifers Fluorescence microscopy Leaf anatomy Phylogeny Taxaceae s.l. 

Notes

Acknowledgements

We would like to thank all botanical institutions and Botanical Gardens (BG) (Atlanta BG, Pinetum Blijdenstein, BG Bochum, BG Bonn, Plantentuin Esveld, BG Marburg, Royal BG Edinburgh, and the Jardin des Plantes Paris), as well as several private collections (Mr. Hubertus Nimsch and Mr. Albrecht Weiss) for their support in providing access to living material. Likewise, we thank the Nationaal Herbarium Nederland in Leiden and the Museum National d’ Historie Naturelle in Paris for their generous access to herbarium material.

Supplementary material

10265_2017_973_MOESM1_ESM.pdf (5.8 mb)
Supplementary material 1 (PDF 5974 KB)

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Copyright information

© The Botanical Society of Japan and Springer Japan KK 2017

Authors and Affiliations

  1. 1.Department for Evolution and Biodiversity of PlantsRuhr-Universität BochumBochumGermany
  2. 2.Botanischer Garten RombergparkDortmundGermany

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