Journal of Plant Research

, Volume 130, Issue 6, pp 1071–1077 | Cite as

Biochemical indicators of root damage in rice (Oryza sativa) genotypes under zinc deficiency stress

Regular Paper

Abstract

Zn deficiency is one of the major soil constraints currently limiting rice production. Although recent studies demonstrated that higher antioxidant activity in leaf tissue effectively protects against Zn deficiency stress, little is known about whether similar tolerance mechanisms operate in root tissue. In this study we explored root-specific responses of different rice genotypes to Zn deficiency. Root solute leakage and biomass reduction, antioxidant activity, and metabolic changes were measured using plants grown in Zn-deficient soil and hydroponics. Solute leakage from roots was higher in sensitive genotypes and linked to membrane damage caused by Zn deficiency-induced oxidative stress. However, total root antioxidant activity was four-fold lower than in leaves and did not differ between sensitive and tolerant genotypes. Root metabolite analysis using gas chromatography–mass spectrometry and high performance liquid chromatography indicated that Zn deficiency triggered the accumulation of glycerol-3-phosphate and acetate in sensitive genotypes, while less or no accumulation was seen in tolerant genotypes. We suggest that these metabolites may serve as biochemical indicators of root damage under Zn deficiency.

Keywords

Zn deficiency Oxidative stress Rice Root solute leakage Acetic acid Glycerol-3-phosphate 

Supplementary material

10265_2017_962_MOESM1_ESM.pdf (517 kb)
Supplementary material 1 (PDF 517 KB)

References

  1. Alloway BJ (2008) Zinc in soils and crop Nutrition, 2nd edn. International Zinc Association, Brussels and International Fertilizer Industry Association, ParisGoogle Scholar
  2. Asada K (2006) Production and scavenging of reactive oxygen species in chloroplasts and their functions. Plant Physiol 141:391–396CrossRefPubMedPubMedCentralGoogle Scholar
  3. Bandyopadhyay T, Mehra P, Hairat S, Giri J (2017) Morpho-physiological and transcriptome profiling reveal novel zinc deficiency-responsive genes in rice. Funct Integr Genom. doi:10.1007/s10142-017-0556-x Google Scholar
  4. Berg MJ, Tymoczko JL, Stryer L (2002) Biochemistry, 5th edn. W H Freeman, New YorkGoogle Scholar
  5. Cakmak I (2000) Possible roles of zinc in protecting plant cells from damage by reactive oxygen species. New Phytol 146:185–205CrossRefGoogle Scholar
  6. Dat J, Vandenabeele S, Vranová E, Van Montagu M, Inzé D, Van Breusegem F (2000) Dual action of the active oxygen species during plant stress responses. Cell Mol Life Sci 57:779–795CrossRefPubMedGoogle Scholar
  7. Dewanto V, Wu X, Liu RH (2002) Processed sweet corn has higher antioxidant activity. J Agric Food Chem 50:4959–4964CrossRefPubMedGoogle Scholar
  8. EI B, Ram PC, Jackson MB, Reuss J, Harren FJ (2003) Dynamic aspects of alcoholic fermentation of rice seedlings in response to anaerobiosis and to complete submergence: relationship to submergence tolerance. Ann Bot 91:279–290CrossRefGoogle Scholar
  9. Estioko LP, Miro B, Baltazar AM, Merca FE, Ismail AM, Johnson DE (2014) Differences in responses to flooding by germinating seeds of two contrasting rice cultivars and two species of economically important grass weeds. AoB Plants 6:plu064CrossRefPubMedPubMedCentralGoogle Scholar
  10. Frei M, Wang Y, Ismail AM, Wissuwa M (2010) Biochemical factors conferring shoot tolerance to oxidative stress in rice grown in low zinc soil. Funct Plant Biol 37:74–84CrossRefGoogle Scholar
  11. Ismail AM, Hall AE (1999) Reproductive-stage heat tolerance, leaf membrane thermostability and plant morphology in Cowpea. Crop Sci 39:1762–1768CrossRefGoogle Scholar
  12. Kimmerer T, Kozlowski TT (1982) Ethylene, ethane, acetaldehyde, and ethanol production by plants under stress. Plant Physiol 69:840–847CrossRefPubMedPubMedCentralGoogle Scholar
  13. Kirk GJD (2004) The biogeochemistry of submerged soils. Wiley, ChichesterCrossRefGoogle Scholar
  14. Lee DJ, Lee JY (2004) Antioxidant activity by DPPH assay. Kor J Crop Sci 49:187–194Google Scholar
  15. Mengel K, Breininger MTh, Bübl W (1984) Bicarbonate, the most important factor inducing iron chlorosis in vine grapes on calcareous soil. Plant Soil 81:333–344CrossRefGoogle Scholar
  16. Miro B, Ismail AM (2013) Tolerance of anaerobic conditions caused by flooding during germination and early growth in rice (Oryza sativa L.). Front Plant Sci 4:269CrossRefPubMedPubMedCentralGoogle Scholar
  17. Mori A, Kirk GJD, Lee JS, Morete MJ, Nanda AK, Johnson-Beebout SE, Wissuwa M (2016) Rice genotype differences in tolerance of zinc-deficient soils: evidence for the importance of root-induced changes in the rhizosphere. Front Plant Sci 6:1160CrossRefPubMedPubMedCentralGoogle Scholar
  18. Nakazono M, Tsuji H, Li Y, Saisho D, Arimura S, Tsutsumi N, Hirai A (2000) Expression of a gene encoding mitochondrial aldehyde dehydrogenase in rice increases under submerged conditions. Plant Physiol 124:587–598CrossRefPubMedPubMedCentralGoogle Scholar
  19. Neue HU, Lantin RS (1994) Micronutrient toxicities and deficiencies in rice. In: Yeo AR, Flowers TJ,(eds) Soil mineral stresses: approaches to crop improvement. Springer, Berlin, pp 175–200CrossRefGoogle Scholar
  20. Ponnamperuma FN (1972) The chemistry of submerged soils. Adv Agron 24:29–96CrossRefGoogle Scholar
  21. Quijano-Guerta C, Kirk GJD, Portugal AM, Bartolome VI, McLaren GC (2002) Tolerance of rice germplasm to zinc deficiency. Field Crops Res 76:123–130CrossRefGoogle Scholar
  22. Ripperger H (1993) Gas chromatography/mass spectrometry of Trimethylsilyl derivatives of Nicotianamine and related amino acids. Biol Mass Spect 22:163–169CrossRefGoogle Scholar
  23. Roessner U, Luedemann A, Brust D, Fiehn O, Linke T, Willmitzer L, Fernie A (2001) Metabolic profiling allows comprehensive phenotyping of genetically or environ-mentally modified plant systems. Plant Cell 13:11–29CrossRefPubMedPubMedCentralGoogle Scholar
  24. Rose MT, Rose TJ, Pariasca-Tanaka J, Widodo, Wissuwa M (2011) Revisiting the role of organic acids in the bicarbonate tolerance of zinc-efficient rice genotypes. Funct Plant Biol 38:493–504Google Scholar
  25. Rose MT, Rose TJ, Pariasca-Tanaka J, Yoshihashi T, Neuweger H, Goesmann A, Frei M, Wissuwa M (2012) Root metabolic response of rice (Oryza sativa L.) genotypes with contrasting tolerance to zinc deficiency and bicarbonate excess. Planta 236:959–973CrossRefPubMedGoogle Scholar
  26. Schraudner M, Möder W, Wiese C, Van Camp W, Inzé D, Langebartels C, Sandermann H (1998) Ozone-induced oxidative burst in the ozone biomonitor plant, tobacco Bel W3. Plant J 16:235–245CrossRefPubMedGoogle Scholar
  27. White JG, Zasoski RJ (1999) Mapping soil micronutrients. Field Crops Res 60:11–24CrossRefGoogle Scholar
  28. Wissuwa M, Ismail AM, Yanagihara S (2006) Effects of zinc deficiency on rice growth and genetic factors contributing to tolerance. Plant Physiol 142:731–741CrossRefPubMedPubMedCentralGoogle Scholar
  29. Yoshida S, Tanaka A (1969) Zinc deficiency of the rice plant in calcareous soils. Soil Sci Plant Nutr 15:75–80CrossRefGoogle Scholar
  30. Yoshida S, Forno DA, Cock JH, Gomez KA (1971) Laboratory manual for physiological studies of rice, 3rd edn. IRRI, Los BañosGoogle Scholar

Copyright information

© The Botanical Society of Japan and Springer Japan KK 2017

Authors and Affiliations

  1. 1.College of AgricultureUniversity of the Philippines Los Baños CollegeLagunaPhilippines
  2. 2.Crop and Environmental Sciences DivisionInternational Rice Research InstituteMetro ManilaPhilippines
  3. 3.Genetics and Biotechnology DivisionInternational Rice Research InstituteMetro ManilaPhilippines
  4. 4.Crop, Livestock and Environment DivisionJapan International Research Centre for Agricultural ScienceTsukubaJapan

Personalised recommendations