Journal of Plant Research

, Volume 130, Issue 6, pp 999–1012 | Cite as

Viridiuvalis adhaerens gen. et sp. nov., a novel colony-forming chlorarachniophyte

  • Takashi Shiratori
  • Sakuya Fujita
  • Tenma Shimizu
  • Takeshi Nakayama
  • Ken-ichiro Ishida
Regular Paper

Abstract

A new chlorarachniophyte, Viridiuvalis adhaerens gen. et sp. nov. was isolated from the mucus on a coral reef from Zanpa Beach, Okinawa, Japan. The main vegetative stage of V. adhaerens consisted of unicellular coccoid cells with cell walls, although sarcinoid colonies and uniflagellate zoospores were also observed. V. adhaerens had chloroplasts with nucleomorphs and pyrenoids that were completely embedded in the chloroplast. A deep plate-like invagination of the periplastidal compartment (PPC) almost partitioned the pyrenoid and chloroplast components, which were surrounded by two membranes. The nucleomorph was positioned in the base of the invagination of the PPC. Molecular phylogenetic analyses using rRNA genes showed that V. adhaerens branched as a sister lineage of the Amorphochlora clade. The sarcinoid colony, pyrenoid embedded in the chloroplast, and nucleomorph located at the base of the deep invagination of the PPC have not been reported in other chlorarachniophytes. Based on these morphological and ultrastructural characteristics and the results of the molecular phylogenetic analyses, we propose V. adhaerens as a new genus and species of chlorarachniophyte.

Keywords

Chlorarachniophyceae Chlorarachniophycota Phylogeny Taxonomy Viridiuvalis adhaerens Ultrastructure 

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Copyright information

© The Botanical Society of Japan and Springer Japan KK 2017

Authors and Affiliations

  • Takashi Shiratori
    • 1
  • Sakuya Fujita
    • 2
  • Tenma Shimizu
    • 3
  • Takeshi Nakayama
    • 1
  • Ken-ichiro Ishida
    • 1
  1. 1.Faculty of Life and Environmental SciencesUniversity of TsukubaTsukubaJapan
  2. 2.Graduate School of Life and Environmental SciencesUniversity of TsukubaTsukubaJapan
  3. 3.Graduate School of Frontier BiosciencesOsaka UniversitySuitaJapan

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