Chloroplast aggregation during the cold-positioning response in the liverwort Marchantia polymorpha
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Under low-light conditions, chloroplasts localize along periclinal cell walls at temperatures near 20 °C, but they localize along anticlinal cell walls near 5 °C. This phenomenon is known as the cold-positioning response. We previously showed that chloroplasts move as aggregates rather than individually during the cold-positioning response in the fern Adiantum capillus-veneris. This observation suggested that chloroplasts physically interact with each other during the cold-positioning response. However, the physiological processes underlying chloroplast aggregation are unclear. In this report, we characterized chloroplast aggregation during the cold-positioning response in the liverwort Marchantia polymorpha. Confocal laser microscopy observations of transgenic liverwort plants expressing a fluorescent fusion protein that localizes to the chloroplast outer envelope membrane (OEP7-Citrine) showed that neighboring chloroplast membranes did not fuse during the cold-positioning response. Transmission electron microscopy analysis revealed that a distance of at least 10 nm was maintained between neighboring chloroplasts during aggregation. These results indicate that aggregated chloroplasts do not fuse, but maintain a distance of at least 10 nm from each other during the cold-positioning response.
KeywordsBryophytes Chloroplast aggregation Chloroplast movement Low temperature Outer envelope membrane Temperature-controlled microscopy
We thank Mr. Koichiro Takaoka (THERMTRON Co. Ltd., Japan) and Mr. Tadao Onuma (Onuma Factory Co. Ltd., Japan; Study Group of Ohtawara Health and Welfare) for their help in developing the temperature-controlled microscope system. We also thank Dr. Takayuki Kohchi (Kyoto University) for providing the Tak-1 and BC3-38 strains. H.T. was supported by a Postdoctoral Fellowship of the Creative Department for Innovation of Utsunomiya University. N.H. was supported by the Hayashi Rheology Memorial Foundation. This work was supported by a Fellowship for Overseas Collaboration Research of the Japanese Society of Plant Physiologists (JSPP) (for collaboration between A.H. and Y.K.), the Japan Society for the Promotion of Science (JSPS) KAKENHI (nos. 23870002 and 26840088 to Y.K.), the JST-ERATO Numata Organelle Reaction Cluster (JPMJER1602 to Y.K.), and Research and Development Grants of the Creative Department for Innovation of Utsunomiya University (Y.K.).
- Kadowaki Y, Sato Y, Ghosh TK, Takezawa D (2015) Inhibition by abscisic acid of cold-induced relocation of chloroplasts in the liverwort Marchantia polymorpha. Cryobiol Cryotechnol 61:145–150Google Scholar
- Qiu YL, Li L, Wang B, Chen Z, Knoop V, Groth-Malonek M, Dombrovska O, Lee J, Kent L, Rest J, Estabrook GF, Hendry TA, Taylor DW, Testa CM, Ambros M, Crandall-Stotler B, Duff RJ, Stech M, Frey W, Quandt D, Davis CC (2006) The deepest divergences in land plants inferred from phylogenomic evidence. Proc Natl Acad Sci USA 103:15511–15516CrossRefPubMedPubMedCentralGoogle Scholar
- Rasband WS (1997–2016) ImageJ. U.S. National Institutes of Health, Bethesda. http://imagej.nih.gov/ij/
- Senn G (1908) Die Gestalts—und Lageveränderung der Pflanzen—Chromatophoren. Wilhelm-Engelmann, LeipzigGoogle Scholar