Journal of Plant Research

, Volume 130, Issue 6, pp 1013–1021 | Cite as

Effects of foliage litter of a pioneer shrub (Artemisia halodendron) on germination from the soil seedbank in a semi-arid sandy grassland in China

  • Yongqing Luo
  • Xueyong Zhao
  • Yuqiang Li
  • Tao Wang
Regular Paper


Vegetation recovery during succession is an important process for ecological restoration of the soil, especially in degraded sandy land. However, the driving mechanisms, such as how a pioneer species competes with other species, is uncertain. In China’s Horqin Sandy Land, Artemisia halodendron is an important shrub that is common on semi-fixed dunes, where it replaces Agriophyllum squarrosum during succession, and is an important indicator species of the second stage of dune stabilization. However, how it outcompetes other species is still unclear. In this study, we conducted a seed bank germination experiment using soil from the native habitats of A. halodendron on semi-fixed dunes. We covered the soil with foliage litter of A. halodendron at a range of concentrations. Seed germination and seedling growth were strongly affected by the foliage litter. Seed germination and seedling growth were not harmed by a low concentration (≤50 g m−2) of the foliage litter but severely inhibited by high concentrations (≥100 g m−2). Strong allelopathy, indicated by decreased germination, increased seedling loss, and decreased plant biomass, appeared during the later stages of germination (after about 20 days of incubation). Our results suggest that as a pioneer shrub during the vegetation succession that occurs during dune stabilization, A. halodendron outcompeted other species through the allelopathic effect of its foliage litter. This helps to explain the patchy distribution and heterogeneity of vegetation communities in the Horqin Sandy Land.


Allelopathy Artemisia halodendron Degraded sandy grassland Seed bank Seedling growth Vegetation recovery 



We thank Drs. Jingdong Bi, Peng Lv and Jing Zhang of the Naiman station for their help in the field and the laboratory. We appreciate valuable comments on our manuscript and assistance from Tonghui Zhang, Yulin Li, Xinping Liu, Xiaoan Zuo, Shaokun Wang, and Jie Lian of the Naiman station. We also thank two anonymous reviewers for their constructive comments on this manuscript. This work was financially supported by the National Natural Science Foundation of China (Nos. 31500369, 31640012) and by the “One Hundred Talents” Program (Y551821001) of the Chinese Academy of Sciences.


  1. Cheng W, Chang XX, Dong HJ, Li DF, Liu JY (2008) Allelopathic inhibitory effect of Myriophyllum aquaticum (Vell.) Verdc. on Microcystis aeruginosa and its physiological mechanism. Acta Ecol Sin 28:2595–2603CrossRefGoogle Scholar
  2. Chu C, Mortimer PE, Wang H, Wang Y, Liu X, Yu S (2014) Allelopathic effects of Eucalyptus on native and introduced tree species. Forest Ecol Manag 323:79–84CrossRefGoogle Scholar
  3. Del Fabbro C, Prati D (2015) The relative importance of immediate allelopathy and allelopathic legacy in invasive plant species. Basic Appl Ecol 16:28–35CrossRefGoogle Scholar
  4. Fernández-Herrera LJ, Band-Schmidt CJ, López-Cortés DJ, Hernández-Guerrero CJ, Bustillos-Guzmán JJ, Núñez-Vázquez E (2016) Allelopathic effect of Chattonella marina var. marina (Raphidophyceae) on Gymnodinium catenatum (Dinophycea). Harm Algae 51:1–9CrossRefGoogle Scholar
  5. Fiorentino A, D’Abrosca B, Esposito A, Izzo A, Pascarella MT, D’Angelo G, Monaco P (2009) Potential allelopathic effect of neo-clerodane diterpenes from Teucrium chamaedrys (L.) on stenomediterranean and weed cosmopolitan species. Biochem Syst Ecol 37:349–353CrossRefGoogle Scholar
  6. Huang WD, Zhao XY, Zhao X, Zhao HL, Wang SK, Lian J (2011) A combined approach using ISSR and ITS analysis for the characterization of Artemisia halodendron from Horqin sandy land, northern China. Biochem Syst Ecol 39:346–351CrossRefGoogle Scholar
  7. Huang G, Zhao XY, Padilla FM, Zhao HL (2012) Fine root dynamics and longevity of Artemisia halodendron reflect plant growth strategy in two contrasting habitats. J Arid Environ 79:1–7CrossRefGoogle Scholar
  8. Li FR, Zhao LY, Zhang H, Zhang TH, Shirato Y (2004) Wind erosion and airborne dust deposition in farmland during spring in the Horqin Sandy Land of eastern Inner Mongolia, China. Soil Till Res 75:121–130CrossRefGoogle Scholar
  9. Li FR, Zhang AS, Duan SS, Kang LF (2005) Patterns of reproductive allocation in Artemisia halodendron inhabiting two contrasting habitats. Acta Oecol 28:57–64CrossRefGoogle Scholar
  10. Li YQ, Zhao HL, Zhao XY, Zhang TH, Chen YP (2006) Biomass energy, carbon and nitrogen stores in different habitats along a desertification gradient in the semiarid Horqin Sandy Land. Arid Land Res Manag 20:43–60CrossRefGoogle Scholar
  11. Li YQ, Zhao HL, Zhao XY, Zhang TH, Li YL, Cui JY (2010) Effects of grazing and livestock exclusion on soil physical and chemical properties in desertified sandy grassland, Inner Mongolia, northern China. Environ Earth Sci 63:771–783CrossRefGoogle Scholar
  12. Li YQ, Zhao XY, Chen YP, Luo YQ, Wang SK (2012a) Effects of grazing exclusion on carbon sequestration and the associated vegetation and soil characteristics at a semi-arid desertified sandy site in Inner Mongolia, northern China. Can J Soil Sci 92:807–819CrossRefGoogle Scholar
  13. Li YQ, Zhou XH, Brandle JR, Zhang TH, Chen YP, Han JJ (2012b) Temporal progress in improving carbon and nitrogen storage by grazing exclosure practice in a degraded land area of China’s Horqin Sandy Grassland. Agric Ecosyst Environ 159:55–61CrossRefGoogle Scholar
  14. Li YQ, Brandle JR, Awada T, Chen YP, Han JJ, Zhang FX, Luo YQ (2013) Accumulation of carbon and nitrogen in the plant–soil system after afforestation of active sand dunes in China’s Horqin Sandy Land. Agric Ecosyst Environ 177:75–84CrossRefGoogle Scholar
  15. Liu B, Liu ZM, Guan DX (2007) Seedling growth variation in response to sand burial in four Artemisia species from different habitats in the semi-arid dune field. Trees 22:41–47CrossRefGoogle Scholar
  16. Liu Y, Li F, Huang QX (2013) Allelopathic effects of gallic acid from Aegiceras corniculatum on Cyclotella caspia. J Environ Sci China 25:776–784CrossRefPubMedGoogle Scholar
  17. Ma JL, Liu ZM, Zeng DH, Liu B (2010) Aerial seed bank in Artemisia species: how it responds to sand mobility. Trees 24(3):435–441CrossRefGoogle Scholar
  18. Miao RH, Jiang DM, Musa A, Zhou QL, Guo MX, Wang YC (2015) Effectiveness of shrub planting and grazing exclusion on degraded sandy grassland restoration in Horqin sandy land in Inner Mongolia. Ecol Eng 74:164–173CrossRefGoogle Scholar
  19. Omezzine F, Haouala R (2013) Effect of Trigonella foenum-graecum L. development stages on some phytochemicals content and allelopathic potential. Sci Hortic Amst 160:335–344CrossRefGoogle Scholar
  20. Pakdel FM, Sim L, Beardall J, Davis J (2013) Allelopathic inhibition of microalgae by the freshwater stonewort, Chara australis, and a submerged angiosperm, Potamogeton crispus. Aquat Bot 110:24–30CrossRefGoogle Scholar
  21. Rawat LS, Maikhuri RK, Negi VS (2013) Inhibitory effect of leachate from Helianthus annuus on germination and growth of Kharif crops and weeds. Acta Ecol Sin 33:245–252CrossRefGoogle Scholar
  22. Silva RMG, Brigatti JGF, Santos VHM, Mecina GF, Silva LP (2013) Allelopathic effect of the peel of coffee fruit. Sci Hortic Amst 158:39–44CrossRefGoogle Scholar
  23. Sohrabi S, Ghanbari A, Mohassel MHR, Gherekhloo J, Vidal RA (2016) Effects of environmental factors on Cucumis melo L. subsp. agrestis var. agrestis (Naudin) Pangalo seed germination and seedling emergence. S Afr J Bot 105:1–8CrossRefGoogle Scholar
  24. Su YZ, Zhang TH, Li YL, Wang F (2005) Changes in soil properties after establishment of Artemisia halodendron and Caragana microphylla on shifting sand dunes in semiarid Horqin Sandy Land, northern China. Environ Manag 36:272–281CrossRefGoogle Scholar
  25. Tesio F, Weston LA, Ferrero A (2011) Allelochemicals identified from Jerusalem artichoke (Helianthus tuberosus L.) residues and their potential inhibitory activity in the field and laboratory. Sci Hortic Amst 129:361–368CrossRefGoogle Scholar
  26. Tesio F, Vidotto F, Ferrero A (2012) Allelopathic persistence of Helianthus tuberosus L. residues in the soil. Sci Hortic Amst 135:98–105CrossRefGoogle Scholar
  27. Vidotto F, Tesio F, Ferrero A (2013) Allelopathic effects of Ambrosia artemisiifolia L. in the invasive process. Crop Prot 54:161–167CrossRefGoogle Scholar
  28. Wang PC, Mo BT, Long ZF, Fan SQ, Wang HH, Wang LB (2016) Factors affecting seed germination and emergence of Sophora davidii. Ind Crop Prod 87:261–265CrossRefGoogle Scholar
  29. Yang L, Han G, Luo YQ, Wang Y, Zhu MN (2016) The intensity of sand drift activities in spring in the downstream of the Laoha River, China. J Arid Land Resour Environ 30:174–179Google Scholar
  30. Zhang CL, Fu SL (2009) Allelopathic effects of eucalyptus and the establishment of mixed stands of eucalyptus and native species. For Ecol Manag 258:1391–1396CrossRefGoogle Scholar
  31. Zhang TH, Zhao HL, Li SG, Li FR, Shirato Y, Ohkuro T, Taniyama I (2004) A comparison of different measures for stabilizing moving sand dunes in the Horqin Sandy Land of Inner Mongolia, China. J Arid Environ 58:203–214CrossRefGoogle Scholar
  32. Zhang JY, Zhao HL, Zhang TH, Zhao XY, Drake S (2005) Community succession along a chronosequence of vegetation restoration on sand dunes in Horqin Sandy Land. J Arid Environ 62:555–566CrossRefGoogle Scholar
  33. Zhang CL, Li XW, Chen YQ, Zhao J, Wan SZ, Lin YB, Fu SL (2016) Effects of Eucalyptus litter and roots on the establishment of native tree species in Eucalyptus plantations in South China. For Ecol Manag 375:76–83CrossRefGoogle Scholar
  34. Zhao HL, Zhao XY, Zhang TH, Wu W (2003) Desertification processes and its restoration mechanisms in the Horqin Sand Land. China Ocean Press, BeijingGoogle Scholar
  35. Zhao HL, Okuro T, Zhou RL, Li YL, Zuo XA (2011) Effects of grazing and climate change on sandy grassland ecosystems in Inner Mongolia. Sci Cold Arid Reg 3:223–232Google Scholar
  36. Zhao HL, Qu H, Zhou RL, Li J, Pan CC, Wang J (2013) Effects of sand burial on growth in two psammophyte seedlings and differences in their physiological responses. Chin J Plant Ecol 37:830–838CrossRefGoogle Scholar
  37. Zuo XA, Zhao HL, Zhao XY, Zhang TH, Guo YR, Wang SK, Drake S (2008a) Spatial pattern and heterogeneity of soil properties in sand dunes under grazing and restoration in Horqin Sandy Land, Northern China. Soil Till Res 99:202–212CrossRefGoogle Scholar
  38. Zuo XA, Zhao XY, Zhao HL, Zhang TH, Guo YR, Li YQ, Huang YX (2008b) Spatial heterogeneity of soil properties and vegetation–soil relationships following vegetation restoration of mobile dunes in Horqin Sandy Land, Northern China. Plant Soil 318:153–167CrossRefGoogle Scholar
  39. Zuo XA, Zhao HL, Zhao XY, Guo YR, Yun JY, Wang SK, Miyasaka T (2009) Vegetation pattern variation, soil degradation and their relationship along a grassland desertification gradient in Horqin Sandy Land, northern China. Environ Geol 58:1227–1237CrossRefGoogle Scholar
  40. Zuo XA, Zhao XY, Zhao HL, Guo YR, Zhang TH, Cui JY (2010) Spatial pattern and heterogeneity of soil organic carbon and nitrogen in sand dunes related to vegetation change and geomorphic position in Horqin Sandy Land, Northern China. Environ Monit Assess 164:29–42CrossRefPubMedGoogle Scholar
  41. Zuo ZJ, Zhang RM, Gao PJ, Wen GS, Hou P, Gao Y (2011) Allelopathic effects of Artemisia frigida Willd. on growth of pasture grasses in Inner Mongolia, China. Biochem Syst Ecol 39:377–383CrossRefGoogle Scholar
  42. Zuo XA, Zhao XY, Wang SK, Li YQ, Lian J, Zhou X (2012) Influence of dune stabilization on relationship between plant diversity and productivity in Horqin Sand Land, Northern China. Environ Earth Sci 67:1547–1556CrossRefGoogle Scholar
  43. Zuo XA, Zhang J, Zhou X, Zhao XY, Wang SK, Lian J, Lv P, Knops J (2015) Changes in carbon and nitrogen storage along a restoration gradient in a semiarid sandy grassland. Acta Oecol 69:1–8CrossRefGoogle Scholar

Copyright information

© The Botanical Society of Japan and Springer Japan 2017

Authors and Affiliations

  • Yongqing Luo
    • 1
  • Xueyong Zhao
    • 1
  • Yuqiang Li
    • 1
  • Tao Wang
    • 2
    • 3
  1. 1.Naiman Desertification Research Station, Northwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesLanzhouPeople’s Republic of China
  2. 2.Key Laboratory of Desert and DesertificationChinese Academy of SciencesLanzhouPeople’s Republic of China
  3. 3.Lanzhou BranchChinese Academy of SciencesLanzhouPeople’s Republic of China

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