Journal of Plant Research

, Volume 130, Issue 4, pp 747–763

Plant dehydrins: shedding light on structure and expression patterns of dehydrin gene family in barley

  • Raha Abedini
  • Farzan GhaneGolmohammadi
  • Reihaneh PishkamRad
  • Ehsan Pourabed
  • Ahad Jafarnezhad
  • Zahra-Sadat Shobbar
  • Maryam Shahbazi
Regular Paper

Abstract

Dehydrins, an important group of late embryogenesis abundant proteins, accumulate in response to dehydration stresses and play protective roles under stress conditions. Herein, phylogenetic analysis of the dehydrin family was performed using the protein sequences of 108 dehydrins obtained from 14 plant species based on plant taxonomy and protein subclasses. Sub-cellular localization and phosphorylation sites of these proteins were also predicted. The protein features distinguishing these dehydrins categories were identified using various attribute weighting and decision tree analyses. The results revealed that the presence of the S motif preceding the K motif (YnSKn, SKn, and SnKS) was more evident and the YnSKn subclass was more frequent in monocots. In barley, as one of the most drought-tolerant crops, there are ten members of YnSKn out of 13 HvDhns. In promoter regions, six types of abiotic stress-responsive elements were identified. Regulatory elements in UTR sequences of HvDhns were infrequent while only four miRNA targets were found. Furthermore, physiological parameters and gene expression levels of HvDhns were studied in tolerant (HV1) and susceptible (HV2) cultivars, and in an Iranian tolerant wild barley genotype (Spontaneum; HS) subjected to gradual water stress and after recovery duration at the vegetative stage. The results showed the significant impact of dehydration on dry matter, relative leaf water, chlorophyll contents, and oxidative damages in HV2 compared with the other studied genotypes, suggesting a poor dehydration tolerance, and incapability of recovering after re-watering in HV2. Under severe drought stress, among the 13 HvDhns genes, 5 and 10 were exclusively induced in HV1 and HS, respectively. The gene and protein structures and the expression patterns of HvDhns as well as the physiological data consistently support the role of dehydrins in survival and recovery of barley plants from drought particularly in HS. Overall, this information would be helpful for functional characterization of the Dhn family in plants.

Keywords

Barley In silico analysis Dehydrin Drought Gene expression 

Abbreviations

CDS

Coding sequence

Dhn

Dehydrin

DW

Dry weight

FW

Fresh weight

HV1

Hordeum vulgare L. cv Yousef

HV2

Hordeum vulgare L. cv Morocco 9–75

HS

Hordeum vulgare L. ssp. Spontaneum

IRES

Internal ribosome entry site

LEA

Late embryogenesis abundant

LT

Leaf temperature

MDA

Malondialdehyde

NLS

Nuclear localization signals

RWC

Relative water content

SPAD

Special products analysis division

TFBS

Transcription factor binding sites

TSS

Transcription start site

TW

Turgid weights

UTR

Untranslated region

WHC

Water holding capacity

Supplementary material

10265_2017_941_MOESM1_ESM.pdf (2.1 mb)
Supplementary material 1 (PDF 2152 KB)

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Copyright information

© The Botanical Society of Japan and Springer Japan 2017

Authors and Affiliations

  1. 1.Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Seed and Plant Improvement Institutes CampusKarajIran
  2. 2.Department of Integrated Biosciences, Graduate School of Frontier SciencesUniversity of TokyoKashiwaJapan

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