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Stoichiometry in aboveground and fine roots of Seriphidium korovinii in desert grassland in response to artificial nitrogen addition

Journal of Plant Research volume 130, pages 689–697 (2017)Cite this article

Abstract

Nitrogen (N) input by atmospheric deposition and human activity enhances the availability of N in various ecosystems, which may further affect N and phosphorus (P) cycling and use by plants. However, the internal use of N, P, and N:P stoichiometry by plants in response to N supply, particularly for grass species in a desert steppe ecosystem, remains unclear. In this work, a field experiment was conducted at an infertile area in a desert steppe to investigate the effects of N fertilizer addition rates on the stoichiometry of N and P in a dominant grass species, Seriphidium korovinii. Results showed that for both aboveground and fine roots of S. korovinii, N inputs exponentially increased the N concentration and N:P ratios while P concentrations decreased. Meanwhile, the relationships between N and P concentrations for both aboveground and fine roots were significantly negative. Furthermore, while the N concentrations in the plants were relatively low, P concentrations were higher than the global means, resulting in a relatively low N:P ratio. These results suggest that the stoichiometric characteristics of N were different from that of P for this desert plant species. Results also show that the intraspecific variations in the main element traits (N, P, and N:P ratios) were consistent at the whole-plant level. Our results also suggest that N should be part of any short-term fertilization plan that is part of a management strategy designed to restore degraded desert grassland. These findings highlight that nutrient addition by atmospheric N deposition and human activity can have significant effects on the internal use of N and P by plants. Therefore, establishing a nutrient-conservation strategy for desert grasslands is important.

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References

  • Aerts R, Cornelissen J, Van Logtestijn R, Callaghan T (2007) Climate change has only a minor impact on nutrient resorption parameters in a high-latitude peatland. Oecologia 151:132–139

    Article  PubMed  CAS  Google Scholar 

  • Drenovsky RE, Richards JH (2004) Critical N:P values: predicting nutrient deficiencies in desert shrublands. Plant Soil 259:59–69

    Article  CAS  Google Scholar 

  • FordeBG 2002 The role of long-distance signalling in plant responses to nitrate and other nutrients. J Exp Bot 53:39–43

    Google Scholar 

  • Freschet GT, Cornelissen JHC, Van Logtestijn RSP, Aerts R (2010) Evidence of the ‘plant economics spectrum’ in a subarctic flora. J Ecol 98:362–373

    Article  Google Scholar 

  • Galloway JN, Dentener FJ, Capone DG, Boyer EW, Howarth RW, Seitzinger SP, Asner GP, Cleveland C, Green P, Holland E (2004) Nitrogen cycles: past, present, and future. Biogeochemistry 70:153–226

    Article  CAS  Google Scholar 

  • Geng Y, Wang L, Jin D, Liu H, He J-S (2014) Alpine climate alters the relationships between leaf and root morphological traits but not chemical traits. Oecologia 175:445–455

    Article  PubMed  Google Scholar 

  • Gordon WS, Jackson RB (2000) Nutrient concentrations in fine roots. Ecology 81:275–280

    Article  Google Scholar 

  • Güsewell S (2004) N: P ratios in terrestrial plants: variation and functional significance. New phytol 164:243–266

    Article  Google Scholar 

  • Han W, Fang J, Guo D, Zhang Y (2005) Leaf nitrogen and phosphorus stoichiometry across 753 terrestrial plant species in China. New Phytol 168:377–385

    Article  PubMed  CAS  Google Scholar 

  • Hendricks JJ, Aber JD, Nadelhoffer KJ, Hallett RD (2000) Nitrogen controls on fine root substrate quality in temperate forest ecosystems. Ecosystems 3:57–69

    Article  CAS  Google Scholar 

  • Holdaway RJ, Richardson SJ, Dickie IA, Peltzer DA, Coomes DA (2011) Species-and community-level patterns in fine root traits along a 120 000-year soil chronosequence in temperate rain forest. J Ecol 99:954–963

    Article  Google Scholar 

  • Holub P, Tůma I (2010) The effect of enhanced nitrogen on aboveground biomass allocation and nutrient resorption in the fern Athyrium distentifolium. Plant Ecol 207:373–380

    Article  Google Scholar 

  • Imsande J and Touraine B (1994) N demand and the regulation of nitrate uptake. Plant Physiol 105:3

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Jackson RB, Mooney H, Schulze E-D (1997) A global budget for fine root biomass, surface area, and nutrient contents. P Natl Acad Sci USA 94:7362–7366

    Article  CAS  Google Scholar 

  • Kerkhoff AJ, Fagan WF, Elser JJ, Enquist BJ (2006) Phylogenetic and growth form variation in the scaling of nitrogen and phosphorus in the seed plants. Am Nat 168:E103–E122

    Article  PubMed  Google Scholar 

  • Kozovits A, Bustamante M, Garofalo C, Bucci S, Franco A, Goldstein G, Meinzer F (2007) Nutrient resorption and patterns of litter production and decomposition in a Neotropical Savanna. Funct Ecol 21:1034–1043

    Article  Google Scholar 

  • Li Y, Song XL, Zhao JN, WangH, BaiL, Yang DL (2015) Responses of plant diversity and primary productivity to nutrient addition in a Stipa baicalensis grassland, China. J Integr Agr 14:2099–2108

    Article  CAS  Google Scholar 

  • Li L, Gao X, Li X, Lin L, Zeng F, Gui D, Lu Y (2016) Nitrogen (N) and phosphorus (P) resorption of two dominant alpine perennial grass species in response to contrasting N and P availability. Environ Exp Bot 127:37–44

    Article  CAS  Google Scholar 

  • Liu G, Freschet GT, Pan X, Cornelissen JH, Li Y, Dong M (2010) Coordinated variation in leaf and root traits across multiple spatial scales in Chinese semi-arid and arid ecosystems. New Phytol 188:543–553

    Article  PubMed  Google Scholar 

  • Lü X-T, Kong D-L, Pan Q-M, Simmons ME, Han X-G (2012a) Nitrogen and water availability interact to affect leaf stoichiometry in a semi-arid grassland. Oecologia 168:301–310

    Article  PubMed  Google Scholar 

  • Lü XT, Freschet GT, Flynn DF, Han XG (2012b) Plasticity in leaf and stem nutrient resorption proficiency potentially reinforces plant–soil feedbacks and microscale heterogeneity in a semi-arid grassland. J Ecol 100:144–150

    Article  Google Scholar 

  • Lü XT, Reed S, Yu Q, He NP, Wang ZW, Han XG (2013) Convergent responses of nitrogen and phosphorus resorption to nitrogen inputs in a semiarid grassland. Global Change Biol 19:2775–2784

    Article  Google Scholar 

  • Menge DNL, Field CB (2007) Simulated global changes alter phosphorus demand in annual grassland. Global Change Biol 13:2582–2591

    Article  Google Scholar 

  • Nadelhoffer KJ (2000) The potential effects of nitrogen deposition on fine-root production in forest ecosystems. New Phytol 147:131–139

    Article  CAS  Google Scholar 

  • Ostertag R (2001) Effects of nitrogen and phosphorus availability on fine-root dynamics in Hawaiian montane forests. Ecology 82:485–499

    Article  Google Scholar 

  • Parkinson J, Allen S (1975) A wet oxidation procedure suitable for the determination of nitrogen and mineral nutrients in biological material. Commun Soil Sci Plant 6:1–11

    Article  CAS  Google Scholar 

  • Sparks DL, Page A, Helmke P, Loeppert R, Soltanpour P, Tabatabai M, Johnston C, Sumner M (1996) Methods of soil analysis. Part 3-chemical methods. Soil Science Society of America Inc, Madison

    Google Scholar 

  • Sterner RW, Elser JJ (2002) Ecological stoichiometry: the biology of elements from molecules to the biosphere. Princeton University, Princeton

    Google Scholar 

  • Van Heerwaarden L, Toet S, Aerts R (2003) Nitrogen and phosphorus resorption efficiency and proficiency in six sub-arctic bog species after 4 years of nitrogen fertilization. J Ecol 91:1060–1070

    Article  Google Scholar 

  • Xia J, Wan S (2008) Global response patterns of terrestrial plant species to nitrogen addition. New Phytol 179:428–439

    Article  PubMed  CAS  Google Scholar 

  • Yu Q, Chen Q, Elser JJ, He N, Wu H, Zhang G, Wu J, Bai Y, Han X (2010) Linking stoichiometric homoeostasis with ecosystem structure, functioning and stability. Ecol Lett 13:1390–1399

    Article  PubMed  Google Scholar 

  • Yuan Z, Chen HY, Reich PB (2011) Global-scale latitudinal patterns of plant fine-root nitrogen and phosphorus. Natcommun 2:344

    CAS  Google Scholar 

Download references

Acknowledgements

This study was supported jointly by the West Light Project of the Chinese Academy of Sciences (2015-XBQN-B-21), the China 1000 Talent Program (Y472171), and National Natural Science Foundation of China (No. 31570002, U1603343). We would like to thank Fengli Chen, Jianrong Lu, Gangliang Tang, Zichun Guo, Yang Ma for assistance with field and laboratory work.

Author contributions

LL is responsible for conception and design, analysis, data collection, manuscript write and critical revision of the article and overall responsibility. XG is responsible for critical revision, DG, BL and BZ are responsible for analysis and data collection, XL is responsible for obtained funding.

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Affiliations

  1. State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, CAS 818 South Beijing Rd., Urumqi, 830011, Xinjiang, China

    Lei Li, Xiaopeng Gao, Dongwei Gui, Bo Zhang & Xiangyi Li

  2. Cele National Station of Observation and Research for Desert-Grassland Ecosystem in Xinjiang, Cele, 848300, Xinjiang, China

    Lei Li, Xiaopeng Gao, Dongwei Gui, Bo Zhang & Xiangyi Li

  3. Key Laboratory of Biogeography and Bioresource in Arid Zone, Chinese Academy of Sciences, Urumqi, 830011, China

    Lei Li, Xiaopeng Gao, Dongwei Gui, Bo Zhang & Xiangyi Li

  4. Department of Soil Science, University of Manitoba, Winnipeg, Canada

    Xiaopeng Gao

  5. College of Resources and Environment, Linyi University, Linyi, 276005, China

    Bo Liu

Authors
  1. Lei Li
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  2. Xiaopeng Gao
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  3. Dongwei Gui
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  4. Bo Liu
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  5. Bo Zhang
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  6. Xiangyi Li
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Correspondence to Xiangyi Li.

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Li, L., Gao, X., Gui, D. et al. Stoichiometry in aboveground and fine roots of Seriphidium korovinii in desert grassland in response to artificial nitrogen addition. J Plant Res 130, 689–697 (2017). https://doi.org/10.1007/s10265-017-0930-8

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  • DOI: https://doi.org/10.1007/s10265-017-0930-8

Keywords

  • Aboveground and fine root
  • Desert grassland
  • Stoichiometry
  • Nutrient concentration
  • N input