A root penetration model of Arabidopsis thaliana in phytagel medium with different strength
- First Online:
- 343 Downloads
Phytagel media were evaluated as systems to mechanically impede roots of A. thaliana. Studying mechanical properties of Phytagel and exploring the root response to mechanical stimulation can facilitate plant culture and plant development. Breaking strengths of 0.5–2.0% phytagel media were tested by uniaxial compression test. Different phytagel concentrations were set to alter the strength of layers in growth medium. Negative correlations were observed between root length, straightness and medium strength. When roots elongated through soft upper-layer (0.6%), penetration ratio decreased with the increase of lower-layer strength (0.6–1.2%) and all roots couldn’t penetrate into lower-layer with concentration ≥1.2%. Roots could grow into soft lower-layer (0.6%) from hard upper-layer (0.6–1.2%), with decreased penetration ratio. When roots grew in soft lower-layer, the growth rate linked with upper-layer strength increased to peak. Roots penetration capability into 1.2% lower-layer was improved by growing plants through moderate layer inserted between soft and hard layer, and roots in 0.8% moderate medium have a significant higher penetration ratio than that in 1.0%. It was concluded that the Phytagel systems studied were suitable for studying the effect of mechanical impedance on the elongation of A. thaliana roots. The medium strength affected root penetration significantly and acclimation can improve root penetration capability.
KeywordsPhytagel medium Mechanical impedance Strength Root penetration Root elongation
- Bochicchio R, Sofo A, Terzano R, Gattullo CE, Amato M, Scopa A (2015) Root architecture and morphometric analysis of Arabidopsis thaliana grown in Cd/Cu/Zn-gradient agar dishes: a new screening technique for studying plant response to metals. Plant Physiol Biochem 91:20–27. doi:10.1016/j.plaphy.2015.03.010 CrossRefPubMedGoogle Scholar
- Darwin C, Darwin F (1972) The power of movement in plants. The works of Charles Darwin, vol 15. AMS Press, New YorkGoogle Scholar
- Perrineau F, Wimalasekera R, Effendi Y, Scherer GF (2016) Inhibition of auxin transport and auxin signaling and treatment with far red light induces root coiling in the phospholipase-A mutant ppla-I-1. Significance for surface penetration? J Plant Physiol 196–197:53–59. doi:10.1016/j.jplph.2016.03.010 CrossRefPubMedGoogle Scholar