Journal of Plant Research

, Volume 130, Issue 2, pp 373–386 | Cite as

Overexpression of the ascorbate peroxidase gene from eggplant and sponge gourd enhances flood tolerance in transgenic Arabidopsis

  • Chih-Ming Chiang
  • Chiu-Chen Chen
  • Shi-Peng Chen
  • Kuan-Hung Lin
  • Li-Ru Chen
  • Yu-Huei Su
  • His-Cheng Yen
Regular Paper


Previously, we found that the flood resistance of eggplant (Solanum melongena) and sponge gourd (Luffa cylindrica) enhanced ascorbate peroxidase (APX) activity under flooding, and consequently, both the SmAPX and LcAPX genes were cloned. In this study, the SmAPX and LcAPX genes were transferred under a ubiquitin promoter to Arabidopsis (At) via Agrobacterium tumefaciens. The expression and amount of APX and APX activities of the SmAPX and LcAPX transgenic lines were significantly higher than those of non-transgenic (NT) plants under a waterlogged condition. Furthermore, the SmAPX, LcAPX, At-sucrose synthases (SUS)-1, phosphoenolpyruvate carboxylase (PEPC), and lactate dehydrogenase (LDH) genes were overexpressed in all transgenic Arabidopsis lines after flooding treatment. Compared to NT plants, the malondialdehyde (MDA) contents and H2O2 accumulation were significantly lower, but germination rates were significantly higher in all transgenic lines with higher APX activity, indicating that the overexpression of SmAPX and LcAPX in Arabidopsis could enhance flood tolerance by eliminating H2O2. Moreover, Arabidopsis seedlings overexpressing SmAPX and LcAPX also displayed greater resistance to flooding and less oxidative injury than NT plants subjected to flooding condition.


Flood tolerance Transgenic Arabidopsis Ascorbate peroxidase 

Supplementary material

10265_2016_902_MOESM1_ESM.docx (18 kb)
Supplementary material 1 (DOCX 18 KB)
10265_2016_902_MOESM2_ESM.docx (423 kb)
Supplementary material 2 (DOCX 422 KB)


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Copyright information

© The Botanical Society of Japan and Springer Japan 2017

Authors and Affiliations

  • Chih-Ming Chiang
    • 1
  • Chiu-Chen Chen
    • 1
  • Shi-Peng Chen
    • 2
  • Kuan-Hung Lin
    • 3
    • 4
  • Li-Ru Chen
    • 4
  • Yu-Huei Su
    • 1
  • His-Cheng Yen
    • 1
  1. 1.Department of BiotechnologyMing Chuan UniversityTaoyuanTaiwan, Republic of China
  2. 2.Graduate Institute of Plant BiologyNational Taiwan UniversityTaipeiTaiwan, Republic of China
  3. 3.Faculty of Applied SciencesTon Duc Thang UniversityHo Chi Minh CityVietnam
  4. 4.Department of Horticulture and BiotechnologyChinese Culture UniversityTaipeiTaiwan, Republic of China

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