Journal of Plant Research

, Volume 129, Issue 5, pp 1005–1010 | Cite as

Identifying sex in non-fertile individuals of the moss Drepanocladus turgescens (Bryophyta: Amblystegiaceae) using a novel molecular approach

  • Lars Hedenäs
  • Helena Korpelainen
  • Irene Bisang
Technical Note


Sex identification before sexual maturity is notoriously difficult in plants with separate sexes, but is crucial to address many life history related issues. To study the performance of the two sexes in the rarely sexually reproducing dioecious moss Drepanocladus turgescens a molecular sex marker is needed. The female-targeting marker previously developed for D. trifarius and D. lycopodioides amplifies for a few D. turgescens males, which can thus not be distinguished from females. In a significant addition to the earlier developed method we sequenced the portion successfully amplified by the primers PT−3f and PT−3r for six females and three males. Differences between males and females were revealed at five sequence positions. Examination of a total of fourteen females and seven marker amplifying males confirm that females and such males differ consistently at these positions. The usefulness of a previous protocol for moss sex identification is thus extended to another dioecious moss by the addition of a step where a portion of the sex-correlated region is sequenced.


Biased sex ratio Genetic sex ratio Molecular sex marker New sequencing step 



We thank K. Mirbakhsh and M. Pietiläinen for their efficient labwork. Two reviewers provided valuable comments to an earlier version of the manuscript.


  1. Barrett SCH, Hough J (2013) Sexual dimorphism in flowering plants. J Exp Bot 64:67–82CrossRefPubMedGoogle Scholar
  2. Bertiller MB, Sain CL, Bisigato AJ, Coronato FR, Ares JO, Graff P (2002) Spatial sex segregation in the dioecious grass Poa ligularis in northern Patagonia: the role of environmental patchiness. Biodivers Conserv 11:69–84CrossRefGoogle Scholar
  3. Bisang I, Hedenäs L (2005) Sex ratio patterns in dioicous bryophytes re-visited. J Bryol 27:207–219CrossRefGoogle Scholar
  4. Bisang I, Hedenäs L (2013) Males are not shy in the wetland moss Drepanocladus lycopodioides. Int J Plant Sci 174:733–739CrossRefGoogle Scholar
  5. Bisang I, Korpelainen H, Hedenäs L (2010) Can the sex-specific molecular marker of Drepanocladus trifarius uncover gender in related species? J Bryol 32:305–308CrossRefGoogle Scholar
  6. Bisang I, Ehrlén J, Persson C, Hedenäs L (2014) Family affiliation, sex ratio and sporophyte frequency in unisexual mosses. Bot J Linn Soc 174:163–172CrossRefGoogle Scholar
  7. Bisang I, Ehrlén J, Korpelainen H, Hedenäs L (2015) No evidence of sexual niche partitioning in a dioecious moss with rare sexual reproduction. Ann Bot 116:771–779. doi: 10.1093/aob/mcv133 CrossRefPubMedPubMedCentralGoogle Scholar
  8. Brzyski JR, Taylor W, McLetchie DN (2014) Reproductive allocation between the sexes, across natural and novel habitats, and its impact on genetic diversity. Evol Ecol 28:247–261CrossRefGoogle Scholar
  9. Cronberg N (2002) Colonization dynamics of the clonal moss Hylocomium splendens on islands in a Baltic land uplift area: reproduction, genet distribution and genetic variation. J Ecol 90:925–935CrossRefGoogle Scholar
  10. During HJ (1992) Ecological classification of bryophytes and lichens. In: Bates JW, Farmer AM (eds) Bryophytes and lichens in a changing environment. Clarendon Press, Oxford, pp 1–31Google Scholar
  11. Hedenäs L, Bisang I (2011) The overlooked dwarf males in mosses: unique among green land plants. PPEES 13:121–135Google Scholar
  12. Hedenäs L, Bisang I (2013) Do male and female plants display different haplotype patterns in the moss Drepanocladus trifarius (Bryophyta, Amblystegiaceae)? Pol Bot J 58:279–285Google Scholar
  13. Hedenäs L, Bisang I, Korpelainen H, Cronholm B (2010) The true sex ratio in European Pseudocalliergon trifarium (Bryophyta: Amblystegiaceae) revealed by a novel molecular approach. Biol J Linn Soc 100:132–140CrossRefGoogle Scholar
  14. Korpelainen H, Bisang I, Hedenäs L, Kolehmainen J (2008) The first sex-specific molecular marker discovered in the moss Pseudocalliergon trifarium. J Hered 99:581–587CrossRefPubMedGoogle Scholar
  15. McDaniel SF, Perroud P-F (2012) Invited perspective: bryophytes as models for understanding the evolution of sexual systems. Bryologist 115:1–11CrossRefGoogle Scholar
  16. McDaniel SF, Willis HJ, Shaw AJ (2007) A linkage map reveals a complex basis for segregation distortion in an interpopulation cross in the moss Ceratodon purpureus. Genetics 176:2489–2500CrossRefPubMedPubMedCentralGoogle Scholar
  17. McLetchie DN (2001) Sex-specific germination response in the liverwort Sphaerocarpos texanus (Sphaerocarpaceae). Bryologist 104:69–71CrossRefGoogle Scholar
  18. Newton ME (1971) A cytological distinction between male and female Mnium undulatum Hedw. Trans Brit Bryol Soc 6:230–243CrossRefGoogle Scholar
  19. Norell TE, Jones KS, Payton AC, McDaniel SF (2014) Meiotic sex ratio variation in natural populations of Ceratodon purpureus (Ditrichaceae). Am J Bot 101:1572–1576CrossRefGoogle Scholar
  20. Obbard DJ, Harris SA, Pannell JR (2006) Sexual systems and population genetic structure in an annual plant: testing the metapopulation model. Am Nat 167:354–366. doi: 10.1086/499546 CrossRefPubMedGoogle Scholar
  21. Shaw AJ, Beer SC (1999) Life history variation in gametophyte populations of the moss Ceratodon purpureus. Am J Bot 86:512–521CrossRefPubMedGoogle Scholar
  22. Stark LR, McLetchie DN, Eppley SM (2010) Sex ratios and the shy male hypothesis in the moss Bryum argenteum (Bryaceae). Bryologist 113:788–797CrossRefGoogle Scholar
  23. Stehlik I, Friedman J, Barrett SCH (2008) Environmental influence on primary sex ratio in a dioecious plant. Proc Natl Acad Sci USA 105:10847–10852CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© The Botanical Society of Japan and Springer Japan 2016

Authors and Affiliations

  • Lars Hedenäs
    • 1
  • Helena Korpelainen
    • 2
  • Irene Bisang
    • 1
  1. 1.Department of BotanySwedish Museum of Natural HistoryStockholmSweden
  2. 2.Department of Agricultural SciencesUniversity of HelsinkiHelsinkiFinland

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