Advertisement

Journal of Plant Research

, Volume 129, Issue 4, pp 667–673 | Cite as

Water entry for the black locust (Robinia pseudoacacia L.) seeds observed by dedicated micro-magnetic resonance imaging

  • Mika Koizumi
  • Hiromi Kano
Technical Note
  • 190 Downloads

Abstract

Water entry at germination for black locust (Robinia pseudoacacia L.) seeds which are known as hard seeds with impermeable seed coat to water, was examined using micro-magnetic resonance imaging (MRI). The MRI apparatus equipped with a low-field (1 T; Tesla) permanent magnet was used, which is open access, easy maintenance, operable and transportable. The excellent point of the apparatus is that T 1-enhancement of water signals absorbed in dry seeds against steeping free water is stronger than the apparatuses with high-field superconducting magnets, which enabled clear detection of water entry. Water hardly penetrated into the seeds for more than 8 h but approximately 60 % of seeds germinated by incubating on wet filter papers for several days. Hot water treatments above 75 °C for 3 min effectively induced water gap; scarification was 70 % at 100 °C and 75 °C, declined to 15 % at 50 °C and decreased further at room temperature. Water entered into the scarified seeds exclusively through the lens, spread along the dorsal side of the seeds and reached the hypocotyl, whereas water migrated slowly through hilum side to radicle within 3 h.

Keywords

Black locust (Robinia pseudoacacia L.) Dedicated MRI Imbibition Seed coat Time-lapse imaging Water entry 

Notes

Acknowledgments

We thank Handling Editor for valuable advice in preparation of the manuscript and Prof. H. Sakio of Niigata University for kind orientation about the ecology of black locust species.

References

  1. Akiyama R, Amada T, Otsubo T (2002) A vegetation change after planting Robinia pseudo-acacia at landslide scar on upper area of Oi River. Bull Tsukuba Univ Forests 18:75–84Google Scholar
  2. Baskin CC (2003) Breaking physical dormancy in seeds—focussing on the lens. New Phytol 158:229–232CrossRefGoogle Scholar
  3. Baskin JM, Baskin CC, Li X (2000) Taxonomy, anatomy and evolution of physical dormancy in seeds. Plant Species Biol 15:139–152CrossRefGoogle Scholar
  4. Dell B (1980) Structure and function of the strophiolar plug in seeds of Albizia lophantha. Am J Bot 67:556–563CrossRefGoogle Scholar
  5. Fukuda K, Utsuzawa S, Sakaue D (2007) Correlation between acoustic emission, water status and xylem embolism in pine wilt disease. Tree Physiol 27:969–976CrossRefPubMedGoogle Scholar
  6. Garnczarska M, Zalewski T, Kempka M (2007) Water uptake and distribution in germinating lupine seeds studied by magnetic resonance imaging and NMR spectroscopy. Physiol Plant 130:23–32CrossRefGoogle Scholar
  7. Gruwel MLH, Latta P, Volotovskyy V, Šramek M, Tomanek B (2004) Magnetic resonance imaging of seeds by use of single point acquisition. J Agric Food Chem 52:4979–4983CrossRefPubMedGoogle Scholar
  8. Heil JR, McCarthy MJ, Özilgen M (1992) Magnetic resonance imaging and modeling of water up-take into dry beans. Lebensm-Wiss Technol 25:280–285Google Scholar
  9. Hu XW, Wang YR, Wu YP, Baskin CC (2009) Role of the lens in controlling water uptake in seeds of two Fabaceae (Papilionoideae) species treated with sulphuric acid and hot water. Seed Sci Res 19:73–80CrossRefGoogle Scholar
  10. Kano H, Koizumi M (2014) Dynamic state of water in excised Ligustrum lucidum branches observed by dedicated micro-magnetic resonance imaging. Plant 2:33–40. doi: 10.11648/j.plant.20140203.12 CrossRefGoogle Scholar
  11. Karaki T, Watanabe Y, Kondo T, Koike T (2012) Strophiole of seeds of the black locust acts as a water gap. Plant Species Biol 27:226–232. doi: 10.1111/j.1442-1984.2011.00343.x CrossRefGoogle Scholar
  12. Kikuchi K, Koizumi M, Ishida N, Kano H (2006) Water uptake by dry beans observed by micro-magnetic resonance imaging. Ann Bot 98:545–553CrossRefPubMedPubMedCentralGoogle Scholar
  13. Koizumi M, Kano H (2014a) Water entry in dry soybeans at imbibition observed by dedicated micro-magnetic resonance imaging. Am J Biol Life Sci 2:6–11. http://www.openscienceonline.com/journal/ajbls
  14. Koizumi M, Kano H (2014b) Lens: Water channel for dry broad bean seeds at germination observed by micro-magnetic resonance imaging. Am J Biol Life Sci 2:37–40. http://www.openscienceonline.com/journal/ajbls
  15. Koizumi M, Naito S, Haishi T, Utsuzawa S, Ishida N, Kano H (2006) Thawing of frozen vegetables observed by a small dedicated MRI for food research. Magn Reson Imaging 24:1111–1119CrossRefPubMedGoogle Scholar
  16. Koizumi M, Kikuchi K, Isobe S, Ishida N, Naito S, Kano H (2008) Role of seed coat in imbibing soybean seeds observed by micro-magnetic resonance imaging. Ann Bot 102:343–352CrossRefPubMedPubMedCentralGoogle Scholar
  17. Maekawa M, Nakagoshi N (1997) Riparian landscape changes over a period of 46 years, on the Azusa River in Central Japan. Landsc Urban Plan 37:37–43CrossRefGoogle Scholar
  18. Manning JC, Van Staden J (1987) The role of the lens in seed imbibition and seedling vigour of Sesbania punicea (Cav.) Benth. (Leguminosae: Papilionoideae). Ann Bot 59:705–713Google Scholar
  19. Masaka K, Yamada K (2009) Variation in germination character of Robinia pseudoacacia L. (Leguminosae) seeds at individual tree level. J Forest Res 14:167–177CrossRefGoogle Scholar
  20. McEntyre E, Ruan R, Fulcher RG (1998) Comparison of water absorption patterns in two barley cultivars, using magnetic resonance imaging. Cereal Chem 75:792–795CrossRefGoogle Scholar
  21. Molina-Cano J-L, Sopena A, Polo JP, Bergareche C, Moralejo MA, Swanston JS, Glidewell SM (2002) Relationships between barley hordeins and malting quality in a mutant of cv. Triumph. II. Genetic and environmental effects on water uptake. J Cereal Sci 36:39–50CrossRefGoogle Scholar
  22. Pietrzak LN, Frégeau-Reid J, Chatson B, Blackwell B (2002) Observations on water distribution in soybean seed during hydration processes using nuclear magnetic resonance imaging. Can J Plant Sci 82:513–519CrossRefGoogle Scholar
  23. Ruan R, Litchfield JB (1992) Determination of water distribution and mobility inside maize kernels during steeping using magnetic resonance imaging. Cereal Chem 69:13–17Google Scholar
  24. Ruan R, Litchfield JB, Eckhoff SR (1992) Simultaneous and nondestructive measurement of transient moisture profiles and structural changes in corn kernels during steeping using microscopic nuclear magnetic resonance imaging. Cereal Chem 69:600–606Google Scholar
  25. Singh DP, Hooda MS, Bonner FT (1991) An evaluation of scarification methods for seeds of two leguminous trees. New Forest 5:139–145CrossRefGoogle Scholar
  26. Song HP, Delwiche SR, Line MJ (1998) Moisture distribution in a mature soft wheat grain by three-dimensional magnetic resonance imaging. J Cereal Sci 27:191–197CrossRefGoogle Scholar
  27. Takahashi A, Koyama H, Takahashi N (2008) Habitat expansion of Robinia pseudoacacia L. and role of seed banks in the Akagawa river basin. J Jpn Forest Soc 90:1–5CrossRefGoogle Scholar
  28. Tamura H, Kaneko T, Makita A (2006) Structure of 50 year old black locust (Robinia pseudoacacia L.) populations planted in a heavily smoke polluted area around Kosaka Mine, Akita Prefecture, northern Japan. J Jpn Soc Reveget Tech 32:432–439CrossRefGoogle Scholar
  29. Terskikh VV, Feurtado JA, Ren C, Abrams SR, Kermode AR (2005) Water uptake and oil distribution during imbibition of seeds of western white pine (Pinus monticola Dougl. ex D. Don) monitored in vivo using magnetic resonance imaging. Planta 221:17–27CrossRefPubMedGoogle Scholar
  30. Umebayashi T, Fukuda K, Haishi T, Sotooka R, Zuhair S, Otsuki K (2011) The developmental process of xylem embolisms in pine wilt disease monitored by multipoint imaging using compact magnetic resonance imaging. Plant Physiol 156:943–951CrossRefPubMedPubMedCentralGoogle Scholar
  31. Wojtyla Ł, Garnczarska M, Zalewski T, Bednarski W, Ratajczak L, Jurga S (2006) A comparative study of water distribution, free radical production and activation of antioxidative metabolism in germinating pea seeds. J Plant Physiol 163:1207–1220CrossRefPubMedGoogle Scholar

Copyright information

© The Botanical Society of Japan and Springer Japan 2016

Authors and Affiliations

  1. 1.Research Institute for Science and EngineeringWaseda UniversityTokyoJapan
  2. 2.Oak-Hill Georgic Patch-Work LaboratoryChibaJapan

Personalised recommendations