Journal of Plant Research

, Volume 129, Issue 2, pp 275–284 | Cite as

miR156 modulates rhizosphere acidification in response to phosphate limitation in Arabidopsis

  • Kai Jian Lei
  • Ya Ming Lin
  • Guo Yong AnEmail author
Regular Paper


Rhizosphere acidification is a general response to Pi deficiency, especially in dicotyledonous plants. However, the signaling pathway underlying this process is still unclear. Here, we demonstrate that miR156 is induced in the shoots and roots of wild type Arabidopsis plants during Pi starvation. The rhizosphere acidification capacity was increased in 35S:MIR156 (miR156 overexpression) plants, but was completely inhibited in 35S:MIM156 (target mimicry) plants. Both 35S:MIR156 and 35S:MIM156 plants showed altered proton efflux and H+-ATPase activity. In addition, significant up-regulation of H+-ATPase activity in 35S:MIR156 roots coupled with increased citric acid and malic acid exudates was observed. qRT-PCR results showed that most H+-ATPase and PPCK gene transcript levels were decreased in 35S:MIM156 plants, which may account for the decreased H+-ATPase activity in 35S:MIM156 plants. MiR156 also affect the root architecture system. Collectively, our results suggest that miR156 regulates the process of rhizosphere acidification in plants.


Arabidopsis thaliana miR156 Rhizosphere acidification Pi deficiency 



We thank Professor Jiawei Wang for providing the 35S:MIR156 and 35S:MIM156 seeds. We also thank Dr. Shuping Xing for providing the PromiR156a:GUS and PromiR156d:GUS seeds. This work was supported by the National Natural Science Foundation of China (31371426, 31570263).

Supplementary material

10265_2015_778_MOESM1_ESM.pdf (389 kb)
Supplementary material 1 (PDF 389 kb)
10265_2015_778_MOESM2_ESM.pdf (193 kb)
Supplementary material 2 (PDF 193 kb)


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Copyright information

© The Botanical Society of Japan and Springer Japan 2015

Authors and Affiliations

  1. 1.College of Life Sciences, State Key Laboratory of Cotton BiologyHenan UniversityKaifengPeople’s Republic of China
  2. 2.Pharmacy College of Henan UniversityKaifengPeople’s Republic of China

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