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Journal of Plant Research

, Volume 128, Issue 4, pp 653–663 | Cite as

Reciprocal translocation identified in Vigna angularis dominates the wild population in East Japan

  • Lixia Wang
  • Shinji Kikuchi
  • Chiaki Muto
  • Ken NaitoEmail author
  • Takehisa Isemura
  • Masao Ishimoto
  • Xuzhen Cheng
  • Akito Kaga
  • Norihiko Tomooka
Regular Paper

Abstract

Using an F2 population derived from cultivated and wild azuki bean, we previously detected a reciprocal translocation and a seed size QTL near the translocation site. To test the hypothesis that the translocation in the cultivated variety contributed to the larger seed size, we performed further linkage analyses with several cross combinations between cultivated and wild azuki beans. In addition, we visually confirmed the translocation by cytogenetic approach using 25 wild and cultivated accessions. As a result, we found the translocation-type chromosomes in none of the cultivated accessions, but in a number of the wild accessions. Interestingly, all the wild accessions with the translocation were originally collected from East Japan, while all the accessions with normal chromosomes were from West Japan or the Sea of Japan-side region. Such biased geographical distribution could be explained by the glacial refugium hypothesis, and supported narrowing down the domestication origin of cultivated azuki bean.

Keywords

Azuki bean Linkage analysis Cytogenetics Reciprocal translocation Wild population 

Notes

Acknowledgments

We are very grateful to Dr. Takaya Iwasaki for his academic adivice about glacial refugium hypothesis. We are also grateful to Hokkaido Prefectural Central Agricultural Experiment Station for supplying seeds of a cultivar ‘Shumari’. This research was partially conducted while the first author was a visiting scholar in NIAS.

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

10265_2015_720_MOESM1_ESM.xlsx (66 kb)
Supplementary material 1 (XLSX 65 kb)

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Copyright information

© The Botanical Society of Japan and Springer Japan 2015

Authors and Affiliations

  • Lixia Wang
    • 1
  • Shinji Kikuchi
    • 2
  • Chiaki Muto
    • 3
  • Ken Naito
    • 3
    Email author
  • Takehisa Isemura
    • 3
  • Masao Ishimoto
    • 4
  • Xuzhen Cheng
    • 1
  • Akito Kaga
    • 3
  • Norihiko Tomooka
    • 3
  1. 1.Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijingChina
  2. 2.Graduate School of HorticultureChiba UniversityChibaJapan
  3. 3.Genetic Resource CenterNational Institute of Agrobiological SciencesTsukubaJapan
  4. 4.Agrogenomics Research CenterNational Institute of Agrobiological SciencesTsukubaJapan

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