Sequence and expression analysis of putative Arachis hypogaea (peanut) Nod factor perception proteins
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Peanut, like most legumes, develops a symbiotic relationship with rhizobia to overcome nitrogen limitation. Rhizobial infection of peanut roots occurs through a primitive and poorly characterized intercellular mechanism. Knowledge of the molecular determinants of this symbiotic interaction is scarce, and little is known about the molecules implicated in the recognition of the symbionts. Here, we identify the LysM extracellular domain sequences of two putative peanut Nod factor receptors, named AhNFR1 and AhNFP. Phylogenetic analyses indicated that they correspond to LjNFR1 and LjNFR5 homologs, respectively. Transcriptional analysis revealed that, unlike LjNFR5, AhNFP expression was not induced at 8 h post bradyrhizobial inoculation. Further examination of AhNFP showed that the predicted protein sequence is identical to GmNFR5 in two positions that are crucial for Nod factor perception in other legumes. Analysis of the AhNFP LysM2 tridimensional model revealed that these two amino acids are very close, delimiting a zone of the molecule essential for Nod factor recognition. These data, together with the analysis of the molecular structure of Nod factors of native peanut symbionts previously reported, suggest that peanut and soybean could share some of the determinants involved in the signalling cascade that allows symbiosis establishment.
KeywordsBradyrhizobia Molecular recognition Nod factor Nod factor receptor proteins Peanut Symbiosis
The authors would like to thank Dr. Peggy Ozias-Akins and Dr. Yuofang Guo (UGA, Athens) for performing BLAST searches and providing peanut contig sequences. This study was financially supported by the SECyT-UNRC, CONICET, Ministerio de Ciencia y Tecnología de Córdoba and ANPCyT grants. V. Muñoz and M. Figueredo are recipients of scholarships from CONICET. F. Ibáñez, J. Angelini, M. L. Tonelli and A. Fabra are members of the Research Career from CONICET.
- Arrighi JF, Barre A, Ben Amor B, Bersoult A, Soriano LC, Mirabella R, de Carvalho-Niebel F, Journet EP, Ghérardi M, Huguet T, Geurts R, Dénarié J, Rougé P, Gough C (2006) The Medicago truncatula lysin motif-receptor-like kinase gene family includes NFP and new nodule-expressed genes. Plant Physiol 142:265–279PubMedCentralPubMedCrossRefGoogle Scholar
- Bonaldi K, Gargani D, Prin Y, Fardoux J, Gully D, Nouwen N, Goormachtig S, Giraud E (2011) Nodulation of Aeschynomene afraspera and A. indica by photosynthetic Bradyrhizobium sp. strain ORS285: the nod-dependent versus the nod-independent symbiotic interaction. Mol Plant Microbe Interact 24:1359–1371PubMedCrossRefGoogle Scholar
- Broghammer A, Krusell L, Blaise M, Sauer J, Sullivan JT, Maolanon N, Vinther M, Lorentzen A, Madsen EB, Jensen KJ, Roepstorff P, Thirup S, Ronson CW, Thygesen MB, Stougaard J (2012) Legume receptors perceive the rhizobial lipochitin oligosaccharide signal molecules by direct binding. Proc Natl Acad Sci USA 109:13859–13864PubMedCentralPubMedCrossRefGoogle Scholar
- Carlson RW, Sanjuan J, Bhat UR, Glushka J, Spaink HP, Wijfjes AH, van Brussel AA, Stokkermans TJ, Peters NK, Stacey G (1993) The structures and biological activities of the lipo-oligosaccharide nodulation signals produced by type I and II strains of B. japonicum. J Biol Chem 268:18372–18381PubMedGoogle Scholar
- Giraud E, Moulin L, Vallenet D, Barbe V, Cytryn E, Avarre JC, Jaubert M, Simon D, Cartieaux F, Prin Y, Bena G, Hannibal L, Fardoux J, Kojadinovic M, Vuillet L, Lajus A, Cruveiller S, Rouy Z, Mangenot S, Segurens B, Dossat C, Franck WL, Chang WS, Saunders E, Bruce D, Richardson P, Normand P, Dreyfus B, Pignol D, Stacey G, Emerich D, Verméglio A, Médigue C, Sadowsky M (2007) Legumes symbioses: absence of Nod genes in photosynthetic bradyrhizobia. Science 316:1307–1312PubMedCrossRefGoogle Scholar
- Hoagland D, Arnon D (1950) Water culture method for growing plants without soil. Calif Agric Exp Stn 347:1–32Google Scholar
- Indrasumunar A, Kereszt A, Searle I, Miyagi M, Li D, Nguyen CD, Men A, Carroll BJ, Gresshoff PM (2010) Inactivation of duplicated nod factor receptor 5 (NFR5) genes in recessive loss-of-function non-nodulation mutants of allotetraploid soybean (Glycine max L. Merr.). Plant Cell Physiol 51:201–214PubMedCrossRefGoogle Scholar
- Lefebvre B, Klaus-Heisen D, Pietraszewska-Bogiel A, Hervé C, Camut S, Auriac MC, Gasciolli V, Nurisso A, Gadella TW, Cullimore J (2012) Role of N-glycosylation sites and CXC motifs in trafficking of Medicago truncatula Nod factor perception protein to the plasma membrane. J Biol Chem 287:10812–10823PubMedCentralPubMedCrossRefGoogle Scholar
- Madsen EB, Antolín-Llovera M, Grossmann C, Ye J, Vieweg S, Broghammer A, Krusell L, Radutoiu S, Jensen ON, Stougaard J, Parniske M (2011) Autophosphorylation is essential for the in vivo function of the Lotus japonicus Nod factor receptor 1 and receptor-mediated signalling in cooperation with Nod factor receptor 5. Plant J 65:404–417PubMedCrossRefGoogle Scholar
- Rey T, Nars A, Bonhomme M, Bottin A, Huguet S, Balzergue S, Jardinaud MF, Bono JJ, Cullimore J, Dumas B, Gough C, Jacquet C (2013) NFP, a LysM protein controlling Nod factor perception, also intervenes in Medicago truncatula resistance to pathogens. New Phytol 198:875–886PubMedCrossRefGoogle Scholar
- Uheda E, Daimon H, Yoshizako F (2001) Colonization and invasion of peanut Arachis hypogea L. roots by gusA-marked Bradyrhizobium sp. Can J Bot 79:733–738Google Scholar