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Journal of Plant Research

, Volume 128, Issue 4, pp 697–707 | Cite as

Isolation and functional characterization of salt-stress induced RCI2-like genes from Medicago sativa and Medicago truncatula

  • Ruicai Long
  • Fan Zhang
  • Zhenyi Li
  • Mingna Li
  • Lili Cong
  • Junmei Kang
  • Tiejun Zhang
  • Zhongxiang Zhao
  • Yan Sun
  • Qingchuan YangEmail author
Regular Paper

Abstract

Salt stress is one of the most significant adverse abiotic factors, causing crop failure worldwide. So far, a number of salt stress-induced genes, and genes improving salt tolerance have been characterized in a range of plants. Here, we report the isolation and characterization of a salt stress-induced Medicago sativa (alfalfa) gene (MsRCI2A), which showed a high similarity to the yeast plasma membrane protein 3 gene (PMP3) and Arabidopsis RCI2A. The sequence comparisons revealed that five genes of MtRCI2(AE) showed a high similarity to MsRCI2A in the Medicago truncatula genome. MsRCI2A and MtRCI2(AE) encode small, highly hydrophobic proteins containing two putative transmembrane domains, predominantly localized in the plasma membrane. The transcript analysis results suggest that MsRCI2A and MtRCI2(AD) genes are highly induced by salt stress. The expression of MsRCI2A and MtRCI2(AC) in yeast mutants lacking the PMP3 gene can functionally complement the salt sensitivity phenotype resulting from PMP3 deletion. Overexpression of MsRCI2A in Arabidopsis plants showed improved salt tolerance suggesting the important role of MsRCI2A in salt stress tolerance in alfalfa.

Keywords

Complementation Medicago Overexpression RCI2 Salt stress Subcellular localization 

Abbreviations

ABA

Abscisic acid

EST

Expressed sequence tag

GFP

Green fluorescent Protein

RCI

Rare cold inducible

PMP

Plasma membrane protein

Notes

Acknowledgments

This work was supported by the China Forage and Grass Research System (CARS-35-04) and Basic Scientific Research Fund of IAS-CAAS (2014ywf-zd-2).

Supplementary material

10265_2015_715_MOESM1_ESM.doc (130 kb)
Supplementary material 1 (DOC 130 kb)
10265_2015_715_MOESM2_ESM.doc (38 kb)
Supplementary material 2 (DOC 38 kb)

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Copyright information

© The Botanical Society of Japan and Springer Japan 2015

Authors and Affiliations

  • Ruicai Long
    • 1
  • Fan Zhang
    • 1
  • Zhenyi Li
    • 1
  • Mingna Li
    • 2
  • Lili Cong
    • 1
  • Junmei Kang
    • 1
  • Tiejun Zhang
    • 1
  • Zhongxiang Zhao
    • 3
  • Yan Sun
    • 2
  • Qingchuan Yang
    • 1
    Email author
  1. 1.Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijingChina
  2. 2.College of Animal Science and TechnologyChina Agriculture UniversityBeijingChina
  3. 3.Cangzhou Technical College and Cangzhou Academy of Agriculture and Forestry SciencesCangzhouChina

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