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Phylogenetic relationships of Petrocoptis A. Braun ex Endl. (Caryophyllaceae), a discussed genus from the Iberian Peninsula

Abstract

Petrocoptis is a small genus of chasmophytic plants endemic to the Iberian Peninsula, with some localized populations in the French Pyrenees. Within the genus, a dozen species have been recognized based on morphological diversity, most of them with limited distribution area, in small populations and frequently with potential threats to their survival. To date, however, a molecular evaluation of the current systematic treatments has not been carried out. The aim of the present study is to infer phylogenetic relationships among its subordinate taxa by using plastidial rps16 intron and nuclear internal transcribed spacer (ITS) DNA sequences; and evaluate the phylogenetic placement of the genus Petrocoptis within the family Caryophyllaceae. The monophyly of Petrocoptis is supported by both ITS and rps16 intron sequence analyses. Furthermore, time estimates using BEAST analyses indicate a Middle to Late Miocene diversification (10.59 Myr, 6.44–15.26 Myr highest posterior densities [HPD], for ITS; 14.30 Myr, 8.61–21.00 Myr HPD, for rps16 intron).

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References

  • Bañares A, Blanca G, Güemes J, Moreno JC, Ortiz S (eds) (2004) Atlas y Libro Rojo de la Flora Vascular Amenazada de España. Dirección General de Conservación de la Naturaleza, Madrid

    Google Scholar 

  • Bañares A, Blanca G, Güemes J, Moreno JC, Ortiz S (eds) (2008) Atlas y Libro Rojo de la Flora Vascular Amenazada de España. Adenda 2008. Dirección General de Medio Natural y Política Forestal (Ministerio de Medio Ambiente, y Medio Rural y Marino)-Sociedad Española de Biología de la Conservación de Plantas, Madrid

  • Bañares A, Blanca G, Güemes J, Moreno JC, Ortiz S (eds) (2010) Atlas y Libro Rojo de la Flora Vascular Amenazada de España. Adenda 2010. Dirección General de Medio Natural y Política Forestal (Ministerio de Medio Ambiente, y Medio Rural y Marino)-Sociedad Española de Biología de la Conservación de Plantas, Madrid

  • Bittrich V (1993) Caryophyllaceae. In: Kubitzki K, Rohwer J, Bittrich V (eds) The families and genera of vascular plants, vol 2., Magnoliid, Hamamelid, and Caryophyllid familiesSpringer, Berlin, pp 206–236

    Google Scholar 

  • Bolòs O, Rivas-Martínez S (1970) Comentarios sobre el Género Petrocoptis. Petrocoptis montsicciana sp. nova. Anal Inst Bot Cavanilles 26:53–60

    Google Scholar 

  • Desfeux C, Lejeune B (1996) Systematics of Euromediterranean Silene (Caryophyllaceae): evidence from a phylogenetic analysis using ITS sequences. C R Acad Sci, Ser III 319:351–358

    CAS  Google Scholar 

  • Drummond AJ, Suchard MA, Xie D, Rambaut A (2012) Bayesian phylogenetics with BEAUti and the BEAST 1.7. Mol Phylogenet Evol 29:1969–1973

    Article  CAS  Google Scholar 

  • Eggens F (2006) Systematics in Sileneae (Caryophyllaceae)—Taxonomy and Philogenetic patterns. PhD thesis, University of Uppsala

  • Fernández Casas J (1973) Estudios sobre el género Petrocoptis A. Br. (Caryophyllaceae). Cuad Cienc Biol 2:43–45

    Google Scholar 

  • Fernández González D, Díaz González TE, Fombella Blanco MA, Valencia Barrera RM (1988) Contribución al estudio polínico del género Petrocoptis A. Br. (Caryophyllaceae). Lagascalia 15:213–220

    Google Scholar 

  • Fior F, Karis PO, Casazza G, Minuto L, Sala F (2006) Molecular phylogeny of the Caryophyllaceae (Caryophyllales) inferred from chloroplast matK and nuclear rDNA ITS sequences. Am J Bot 93:399–411

    Article  CAS  PubMed  Google Scholar 

  • Frajman B, Oxelman B (2007) Reticulate phylogenetics and phytogeographical structure of Heliosperma (Silene, Cariophyllaceae) inferred from chloroplast and nuclear DNA sequences. Mol Phylogenet Evol 43:140–155

    Article  CAS  PubMed  Google Scholar 

  • Frajman B, Eggens F, Oxelman B (2009) Hybrid origins and homoploid reticulate evolution within Heliosperma (Sileneae, Caryophyllaceae)—a multigene phylogenetic approach with relative dating. Syst Biol 58:328–345

    Article  CAS  PubMed  Google Scholar 

  • Greenberg AK, Donoghue MJ (2011) Molecular systematics and character evolution in Caryophyllaceae. Taxon 60:1637–1652

    Google Scholar 

  • Greuter W (1995) Silene (Caryophyllaceae) in Greece: a subgeneric and sectional classification. Taxon 44:543–581

    Article  Google Scholar 

  • Greuter W, Burdet HM, Long G (1984) Med-Checklist, 1. Conservatoire et Jardin Botanique de la Ville Genève/Botanischer Garten & Botanisches Museum Berlin-Dahlem, Geneve & Berlin

  • Harbaugh DT, Nepokroeff M, Rabeler RK, McNeill J, Zimmer EA, Wagner WL (2010) A new lineage-based tribal classification of the family Caryophyllaceae. Int J Plant Sci 171:185–198

    Article  CAS  Google Scholar 

  • Huson DH, Bryant D (2006) Application of phylogenetic networks in evolutionary studies. Mol Phylogenet Evol 23:254–267

    Article  CAS  Google Scholar 

  • Huson DH, Scornavacca C (2010) A survey of combinatorial methods for phylogenetic networks. Genome Biol Evol 3:23–35

    Article  PubMed Central  PubMed  Google Scholar 

  • Huson DH, Rupp R, Scornavacca C (2010) Phylogenetic networks. Concepts, algorithms and applications. Cambridge University Press, Cambridge

    Book  Google Scholar 

  • Jordan GJ, Macphail MK (2003) A Middle-Late Eocene inflorescence of Caryophyllaceae from Tasmania, Australia. Am J Bot 90:761–768

    Article  PubMed  Google Scholar 

  • Laínz M, Muñoz-Garmendia F (1990) Caryophyllaceae. In: Castroviejo S, Laínz M, López González G, Monstserrat P, Muñoz Garmendia F, Paiva J, Villar L (eds) Flora Iberica, vol 2. Consejo Superior de Investigaciones Cientifícas, Madrid, pp 98–464

  • Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) ClustalW and ClustalX version 2. Bioinformatics 23:2947–2948

    Article  CAS  PubMed  Google Scholar 

  • Mayol M (1998) Biosistemática y evolución en el género Petrocoptis A. Braun (Caryophyllaceae). PhD Thesis, University of Valencia

  • Mayol M, Roselló JA (2001) Seed isozyme variation in Petrocoptis A. Braun (Caryophyllaceae). Biochem Syst Ecol 29:379–392

    Article  CAS  PubMed  Google Scholar 

  • Mayol M, Rosselló JA (1999) A synopsis of Silene subgenus Petrocoptis (Caryophyllaceae). Taxon 48:471–482

    Article  Google Scholar 

  • Mayol M, Rosselló JA (2000) Two new combinations in Silene (Caryophyllaceae). Anales Jard Bot Madrid 57:404

    Google Scholar 

  • Mayol M, Rosselló JA (2006) Hybridization studies in Silene Subgen. Petrocoptis (Caryophyllaceae). Folia Geobot 41:203–212

    Article  Google Scholar 

  • Mayol M, Cubas P, Pardo C, Rosselló JA (2000) Taxonomic usefulness of pollen features in Petrocoptis (Caryophyllaceae): a reevaluation. Isr J Plant Sci 48:1–6

    Article  Google Scholar 

  • Montserrat P, Fernández Casas J (1990) Petrocoptis A. Braun. In: Castroviejo S, Laínz M, López González G, Monstserrat P, Muñoz Garmendia F, Paiva J, Villar L (eds) Flora Iberica, vol 2 Consejo Superior de Investigaciones Cientifícas, Madrid, pp 304–312

  • Montserrat P, Montserrat JM (1978) El Petrocoptis crassifolia, su variabilidad y distribución. Doc Phytosociol n.s. 2:321–328

  • Moreno JC (coord) (2008) Lista Roja 2008 de la Flora Vascular Española. Dirección General de Medio Natural y Política Forestal (Ministerio de Medio Ambiente, y Medio Rural y Marino, y Sociedad Española de Biología de la Conservación de Plantas), Madrid

  • Moreno JC (coord) (2011) Lista Roja de la Flora Vascular Española. Actualización con los datos del Adenda 2010 al Atlas y Libro Rojo de la Flora Vascular Amenazada. Dirección General de Conservación de la Naturaleza y Sociedad Española de Biología de la Conservación de Plantas, Madrid

  • Nylander JAA (2004) MrModeltest v2. Program distributed by the author. Evolutionary Biology Centre, Uppsala University, Sweden

    Google Scholar 

  • Oxelman B, Lidén M (1995) Generic boundaries in the tribe Sileneae (Caryophyllaceae) as inferred from nuclear rDNA sequences. Taxon 44:525–542

    Article  Google Scholar 

  • Oxelman B, Lidén M, Berglund D (1997) Chloroplast rps16 intron phylogeny of the tribe Sileneae (Caryophyllaceae). Pl Syst Evol 206:393–410

    Article  Google Scholar 

  • Oxelman B, Lidén M, Rabeler RK, Popp M (2001) A revised generic classification of the tribe Sileneae (Caryophyllaceae). Nord J Bot 20:743–748

    Article  Google Scholar 

  • Popp M, Oxelman B (2007) Origin and evolution of Noth American polyploid Silene (Caryophyllaceae). Am J Bot 94:330–349

    Article  CAS  PubMed  Google Scholar 

  • Popp M, Erixon P, Eggens F, Oxelman B (2005) Origin and evolution of a circumpolar polyploidy species complex in Silene (Caryophyllaceae) inferred from low-copy nuclear RNA polymerase introns, rDNA, and chloroplast DNA. Syst Bot 30:302–313

    Article  Google Scholar 

  • Rambaut A, Drummond AJ (2007) Tracer v1.4. Program distributed by the authors. http://beast.bio.ed.ac.uk/Tracer. Accessed 17 Dec. 2013

  • Rambaut A, Drummond AJ (2008) FigTree. Program distributed by the authors. http://tree.bio.ed.ac.uk/software/figtree/. Accessed 17 Dec. 2013

  • Rohrbach P (1869) Monographie der Gattung Silene. W. Engelmann, Leipzig

    Google Scholar 

  • Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 61:539–542

    Article  PubMed Central  PubMed  Google Scholar 

  • Rothmaler W (1941) Monographie der Gattung Petrocoptis. A Br Bot Jahrb Sist 72:117–130

    Google Scholar 

  • Rothmaler W (1964) Petrocoptis A Braun. In: Tutin TG, Heywood VH, Burges NA, Valentine DH, Walters SM, Webb DA (eds) Flora Europaea, vol 1, 2nd edn. Cambridge University Press, Cambridge, pp 157–158

    Google Scholar 

  • Sloan DB, Oxelman B, Rautenberg A, Taylor DR (2009) Phylogenetic analysis of mitochondrial substitution rate variation in the angiosperm tribe Sileneae. BMC Evol Biol 9:260

    Article  PubMed Central  PubMed  Google Scholar 

  • Smissen RD, Clement JC, Garnock-Jones PJ, Chambers GK (2002) Subfamilial relationships within Caryophyllaceae as inferred from 5′ ndhF sequences. Am J Bot 89:1336–1341

    Article  CAS  PubMed  Google Scholar 

  • Walters SM (1993) Caryophyllaceae. In: Tutin TG, Heywood VH, Burges NA, Valentine DH, Walters SM, Webb DA (eds) Flora Europaea, vol 1, 2nd edn. Cambridge University Press, Cambridge, pp 139–246

    Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal DNA for phylogenetics. In: Innis MA, Gelfund DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315–322

    Chapter  Google Scholar 

  • Williams FN (1896) A revision of the genus Silene L. J Linn Soc, Bot 32:1–196

    Article  Google Scholar 

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Acknowledgments

The authors thank René Pérez for laboratory assistance. We would like to thank Álvaro Bueno, Candela Cuesta, Daniel Goñi, Francisco Javier Fernández Casas, Itziar García, Javier Loidi, José Luis Benito, Juan Antonio Campos, Mercedes Herrera and Miren Idoia Biurrun for their help and critical reading of the manuscript.

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Correspondence to Eduardo Cires.

Appendices

Appendix 1

See Table 3.

Table 3 Genera and type species for the family Caryophyllaceae used to estimate divergence times of Petrocoptis

Appendix 2

See Table 4.

Table 4 Estimated divergence times of Petrocoptis and related lineages from two different data sets (ITS, rps16) implemented in BEAST. A calibration time of 34 million years (Myr) for the split between subfamilies Alsinoideae and Caryophylloideae was used

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Cires, E., Prieto, J.A.F. Phylogenetic relationships of Petrocoptis A. Braun ex Endl. (Caryophyllaceae), a discussed genus from the Iberian Peninsula. J Plant Res 128, 223–238 (2015). https://doi.org/10.1007/s10265-014-0691-6

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  • DOI: https://doi.org/10.1007/s10265-014-0691-6

Keywords

  • BEAST
  • Iberian Peninsula
  • ITS
  • Phylogeny
  • Petrocoptis
  • rps16 intron