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Changes in C–N metabolism under elevated CO2 and temperature in Indian mustard (Brassica juncea L.): an adaptation strategy under climate change scenario

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Abstract

The present study was performed to investigate the possible role of carbon (C) and nitrogen (N) metabolism in adaptation of Indian mustard (Brassica juncea L.) growing under ambient (370 ± 15 ppm) and elevated CO2 (700 ± 15 ppm), and jointly in elevated CO2 and temperature (30/22 °C for day/night). The key enzymes responsible for C–N metabolism were studied in different samples of Brassica juncea L. collected from ambient (AMB), elevated (ELE) and ELExT growth conditions. Total percent amount of C and N in leaves were particularly estimated to establish a clear understanding of aforesaid metabolism in plant adaptation. Furthermore, key morphological and physiological parameters such as plant height, leaf area index, dry biomass, net photosynthetic rate, stomatal conductance, transpiration, total protein and chlorophyll contents were also studied in relation to C/N metabolism. The results indicated that the C-metabolizing enzymes, such as (ribulose-1,5-bisphosphate carboxylase/oxygenase, phosphoenolpyruvate carboxylase, malate dehydrogenase, NAD-malic enzyme, NADP-malic enzyme and citrate synthase) and the N-metabolizing enzymes, such as (aspartate amino transferase, glutamine synthetase, nitrate reductase and nitrite reductase) showed significantly (P < 0.05) higher activities along with the aforesaid physiological and biochemical parameters in order of ELE > ELExT > AMB growth conditions. This is also evident by significant (P < 0.05) increase in percent contents of C and N in leaves as per said order. These findings suggested that improved performance of C–N metabolism could be a possible approach for CO2 assimilation and adaptation in Brassica juncea L. against elevated CO2 and temperature prevailing in climate change scenarios.

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References

  • Arnon DI (1949) Copper enzymes in isolated chloroplasts: polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Ashton AR, Burnell JN, Furbank RT, Jenkins CLD, Hatch MD (1990) Enzymes of C4 photosynthesis. Methods Plant Biochem 3:39–72

    Article  CAS  Google Scholar 

  • Aslam M, Huffaker C (1989) Role of nitrate and nitrite in the induction of nitrite reductase in leaves of barley seedlings. Plant Physiol 91:1152–1156

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Aubry S, Brown NJ, Hibberd JM (2011) The role of proteins in C3 plants prior to their recruitment into the C4 pathway. J Exp Bot 62:3049–3059

    Article  PubMed  CAS  Google Scholar 

  • Bogin E, Wallace A (1969) Citrate synthase from lemon fruit. Method Enzymol 13:19–22

    Article  CAS  Google Scholar 

  • Boomiraj K, Chakrabarti B, Aggarwal PK, Choudhary R, Chander S (2010) Assessing the vulnerability of Indian mustard to climate change. Agric Ecosyst Environ 138:265–273

    Article  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  PubMed  CAS  Google Scholar 

  • Britto DT, Kronzucker HJ (2005) Nitrogen acquisition, PEP carboxylase, and cellular pH homeostasis: new views on old paradigms. Plant Cell Environ 28:1396–1409

    Article  CAS  Google Scholar 

  • Brulfert J, Güclü S, Taybi T, Pierre JN (1993) Enzymatic responses to water stress in detached leaves of the CAM plant Kalanchoë blossfeldiana. J Plant Physiol 133:222–227

    Article  Google Scholar 

  • Carmen Antolín M, Laura Fiasconaro M, Sánchez-Díaz M (2010) Relationship between photosynthetic capacity, nitrogen assimilation and nodule metabolism in alfalfa (Medicago sativa) grown with sewage sludge. J Hazard Mater 182:210–216

    Article  PubMed  Google Scholar 

  • Davies DD (1969) Malate dehydrogenase from pea epicotyls. Methods Enzymol 13:148–150

    Article  CAS  Google Scholar 

  • Geiger M, Haake V, Ludewig F, Sonnewald V, Stitt M (1999) The nitrate and ammonium nitrate supply have a major influence on the response of photosynthesis, carbon metabolism, nitrogen metabolism and growth to elevated carbon dioxide in tobacco. Plant Cell Environ 22:1177–1199

    Article  Google Scholar 

  • Gerard VA, Driscoll T (1996) A Spectrophotometric assay for Rubisco activity: application to the kelp Laminaria saccharina and implications for radiometric assays. J Phycol 32:880–884

    Article  CAS  Google Scholar 

  • Harley S (1993) Use of a simple colorimetric assay to determine conditions for induction of nitrate reductase in plants. Am Biol Teach 55:161–164

    Article  Google Scholar 

  • Hiscox JD, Israeclstam GF (1979) A method for interaction of chlorophyll from leaf tissue without maceration. Can J Bot 57:1332–1334

    Article  CAS  Google Scholar 

  • Högy P, Keck M, Niehaus K, Franzaring J, Fangmeier A (2010) Effects of atmospheric CO2 enrichment on biomass, yield and low molecular weight metabolites in wheat grain. J Cereal Sci 52:215–220

    Article  Google Scholar 

  • Ireland RJ, Joy K (1990) Aminotransferases. Methods Plant Biochem 3:277–286

    Article  CAS  Google Scholar 

  • Kumar N, Kumar S, Vats SK, Ahuja PS (2006) Effect of altitude on primary products of photosynthesis and the associated enzymes in barley and wheat. Photosynth Res 88:63–71

    Article  PubMed  CAS  Google Scholar 

  • Lancien M, Gadal P, Hodges M (2000) Enzyme redundancy and the importance of 2-oxoglutarate in higher plant ammonium assimilation. Plant Physiol 123:817–824

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Lawlor DW (2002) Carbon and nitrogen assimilation in relation to yield: mechanisms are the key to understanding production systems. J Exp Bot 53:773–787

    Article  PubMed  CAS  Google Scholar 

  • Lea PJ, Blackwell RD, Chen FL, Hecht U (1990) Enzymes of ammonia assimilation. Methods Plant Biochem 3:257–276

    Article  CAS  Google Scholar 

  • Lilley RMcC, Walker DA (1974) An improved spectrophotometric assay for ribulosebisphosphate carboxylase. Biochim Biophys Acta 358:226–229

    Article  PubMed  CAS  Google Scholar 

  • Morison JIL, Lawlor DW (1999) Interactions between increasing CO2 concentration and temperature on plant growth. Plant Cell Environ 22:659–682

    Article  CAS  Google Scholar 

  • Nunes-Nesi A, Fernie AR, Mark S (2010) Metabolic and signaling aspects underpinning the regulation of plant carbon nitrogen interactions. Mol Rep 3:973–996

    CAS  Google Scholar 

  • Pandey OP, Bhadula SK, Purohit AN (1984) Changes in the activity of some photosynthetic and photorespiratory enzymes in Selinum vaginatum Clarke grown at two altitudes. Photosynthetica 18:153–155

    CAS  Google Scholar 

  • Pierce JW, Mucurry SD, Mulligan RM, Tolbert NE (1982) Activation and assay of ribulose 1,5-bisphosphate carboxylase/oxygenase. Methods Enzymol 89:47–55

    Article  PubMed  CAS  Google Scholar 

  • Pyankov VI, Artyusheva EG, Edwards GE, Black CC, Soltis PS (2001) Phylogenetic analysis of tribe salsoleae (Chenopodiaceae) based on ribosomal ITS sequences: implications for the evolution of photosynthesis types. Am J Bot 88:1189–1198

    Article  PubMed  CAS  Google Scholar 

  • Ramirez JN, Delcampo FF, Paneque A, Losada M (1966) Ferredoxin nitrite reductase from spinach. Biochem Biophys Acta 118:58–71

    PubMed  CAS  Google Scholar 

  • Reyss A, Prioul JL (1975) Carbonic anahydrase and carboxylase activities from plants (Lolium multiflorum) adapted to different light regimes. Plant Sci Lett 5:189–195

    Article  CAS  Google Scholar 

  • Sakata T, Nakano T, Yokoi Y (2006) Altitudinal changes in Rubisco and APX activities in Aconogonum weyrichii in the alpine region of Mt. Fuji. Polar Biosci 19:115–122

    CAS  Google Scholar 

  • Shimono A, Zhou H, Shen H, Hirota M, Ohtsuka T, Tang Y (2010) Patterns of plant diversity at high altitudes on the Qinghai-Tibetan Plateau. J Plant Ecol 3:1–7

    Article  Google Scholar 

  • Singh S, Bhatia A, Tomer R, Kumar V, Singh B, Singh SD (2013) Synergistic action of tropospheric ozone and carbon dioxide on yield and nutritional quality of Indian mustard (Brassica juncea (L.) Czern.). Environ Monit Assess 185:6517–6529

    Article  PubMed  CAS  Google Scholar 

  • Stitt M, Krapp A (1999) The interaction between elevated carbon dioxide and nitrogen nutrition: the physiological and molecular background. Plant Cell Environ 22:583–621

    Article  CAS  Google Scholar 

  • Streb P, Shang W, Feierabend J, Bligny R (1998) Divergent strategies of photoprotection in high-mountain plants. Planta 207:313–324

    Article  CAS  Google Scholar 

  • Tang S, Xi L, Zheng J, Li H (2003) Response to elevated CO2 of Indian mustard and Sunflower growing on copper contaminated soil. Bull Environ Contam Toxicol 71:988–997

    Article  PubMed  CAS  Google Scholar 

  • Uprety DC, Rabha BK (1999) Effect of elevated CO2 and moisture stress on the carbon and nitrogen contents in Brassica Juncea. Biol Plant 42:133–136

    Article  Google Scholar 

  • Wang R, Xing X, Crawford N (2007) Nitrite acts as a transcriptome signal at micromolar concentrations in Arabidopsis roots. Plant Physiol 145:1735–1745

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Wang L, Pedas P, Eriksson D, Schjoerring JK (2013) Elevated atmospheric CO2 decreases the ammonia compensation point of barley plants. J Exp Bot 64:2713–2724

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Yang X, Wang X, Wei M, Hikosaka S, Goto E (2010) Response of ammonia assimilation in cucumber seedlings to nitrate stress. J Plant Biol 53:173–179

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The first author expresses his deep gratitude to the Department of Botany, University of Delhi, Delhi-110007, India for providing the research and development grant to continue the research activity. Prof. R.R. Singh, Department of Botany, University of Lucknow-226001, India is gratefully acknowledged for his valuable suggestions and support in the present experiment.

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Authors and Affiliations

  1. Department of Botany, University of Delhi, Delhi, 110007, India

    Chandra Shekhar Seth

  2. CSIR-Indian Institute of Toxicology Research, Lucknow, 226001, India

    Virendra Misra

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  1. Chandra Shekhar Seth
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  2. Virendra Misra
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Correspondence to Chandra Shekhar Seth.

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Seth, C.S., Misra, V. Changes in C–N metabolism under elevated CO2 and temperature in Indian mustard (Brassica juncea L.): an adaptation strategy under climate change scenario. J Plant Res 127, 793–802 (2014). https://doi.org/10.1007/s10265-014-0664-9

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  • DOI: https://doi.org/10.1007/s10265-014-0664-9

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