A promoter analysis of MOTHER OF FT AND TFL1 1 (JcMFT1), a seed-preferential gene from the biofuel plant Jatropha curcas
- 836 Downloads
MOTHER OF FT AND TFL1 (MFT)-like genes belong to the phosphatidylethanoamine-binding protein (PEBP) gene family in plants. In contrast to their homologs FLOWERING LOCUS T (FT)-like and TERMINAL FLOWER 1 (TFL1)-like genes, which are involved in the regulation of the flowering time pathway, MFT-like genes function mainly during seed development and germination. In this study, a full-length cDNA of the MFT-like gene JcMFT1 from the biodiesel plant Jatropha curcas (L.) was isolated and found to be highly expressed in seeds. The promoter of JcMFT1 was cloned and characterized in transgenic Arabidopsis. A histochemical β-glucuronidase (GUS) assay indicated that the JcMFT1 promoter was predominantly expressed in both embryos and endosperms of transgenic Arabidopsis seeds. Fluorometric GUS analysis revealed that the JcMFT1 promoter was highly active at the mid to late stages of seed development. After seed germination, the JcMFT1 promoter activity decreased gradually. In addition, both the JcMFT1 expression in germinating Jatropha embryos and its promoter activity in germinating Arabidopsis embryos were induced by abscisic acid (ABA), possibly due to two ABA-responsive elements, a G-box and an RY repeat, in the JcMFT1 promoter region. These results show that the JcMFT1 promoter is seed-preferential and can be used to control transgene expression in the seeds of Jatropha and other transgenic plants.
KeywordsABA MOTHER OF FT AND TFL1 (MFT) Physic nut Promoter Seed
This work was supported by grants from the Top Science and Technology Talents Scheme of Yunnan Province (2009CI123), the Natural Science Foundation of Yunnan Province (2011FA034) and the CAS 135 program (XTBG-T02) to Z.-F. Xu. The authors gratefully acknowledge the Central Laboratory of the Xishuangbanna Tropical Botanical Garden for providing the research facilities.
- Albani D, Hammond-Kosack MC, Smith C, Conlan S, Colot V, Holdsworth M, Bevan MW (1997) The wheat transcriptional activator SPA: a seed-specific bZIP protein that recognizes the GCN4-like motif in the bifactorial endosperm box of prolamin genes. Plant Cell 9:171–184PubMedCentralPubMedCrossRefGoogle Scholar
- Argollo Marques D, Siqueira W, Colombo C, Ferrari R (2013) Breeding and Biotechnology of Jatropha curcas. In: Bahadur B, Sujatha M, Carels N (eds) Jatropha, Challenges for a New Energy Crop. Volume 2, Genetic improvement and biotechnology. Springer New York, pp 457–478. doi: 10.1007/978-1-4614-4915-7_23
- Chakrabarti PP, Prasad RBN (2012) Biodiesel Production from Jatropha curcas Oil. In: Carels N, Sujatha M, Bahadur B (eds) Jatropha, challenges for a new energy crop vol 1: farming, economics and biofuel. Springer New York, pp 463–490. doi: 10.1007/978-1-4614-4806-8_25
- Chikara J, Prakash A, Mastan SG, Ghosh A (2013) Genetic Improvement in Jatropha curcas Through Selection and Breeding. In: Bahadur B, Sujatha M, Carels N (eds) Jatropha, Challenges for a New Energy Crop. Volume 2: Genetic Improvement and Biotechnology. Springer New York, pp 119–133. doi: 10.1007/978-1-4614-4915-7_8
- Ezcurra I, Ellerström M, Wycliffe P, Stålberg K, Rask L (1999) Interaction between composite elements in the napA promoter: both the B-box ABA-responsive complex and the RY/G complex are necessary for seed-specific expression. Plant Mol Biol 40:699–709. doi: 10.1023/A:1006206124512 PubMedCrossRefGoogle Scholar
- Ezcurra I, Wycliffe P, Nehlin L, Ellerström M, Rask L (2000) Transactivation of the Brassica napus napin promoter by ABI3 requires interaction of the conserved B2 and B3 domains of ABI3 with different cis-elements: B2 mediates activation through an ABRE, whereas B3 interacts with an RY/G-box. Plant J 24:57–66PubMedCrossRefGoogle Scholar
- Heller J (1996) Physic nut Jatropha curcas L. Promoting the conservation and use of underutilized and neglected crops 1. International Plant Genetic Resources Institute, Rome, ItalyGoogle Scholar
- Kobayashi Y, Kaya H, Goto K, Iwabuchi M, Araki T (1999) A pair of related genes with antagonistic roles in mediating flowering signals. Sci Signal 286:1960–1962Google Scholar
- Kumar N, Reddy M, Sujatha M (2013) Genetic transformation of jatropha curcas: current status and future prospects. In: Bahadur B, Sujatha M, Carels N (eds.) Jatropha, challenges for a new energy crop. Volume 2: Genetic improvement and biotechnology. Springer New York, pp 535–546. doi: 10.1007/978-1-4614-4915-7_28
- Le BH, Cheng C, Bui AQ, Wagmaister JA, Henry KF, Pelletier J, Kwong L, Belmonte M, Kirkbride R, Horvath S (2010) Global analysis of gene activity during Arabidopsis seed development and identification of seed-specific transcription factors. Proc Natl Acad Sci USA 107:8063–8070PubMedCentralPubMedCrossRefGoogle Scholar
- Pan JL, Fu QT, Xu ZF (2010) Agrobacterium tumefaciens-mediated transformation of biofuel plant Jatropha curcas using kanamycin selection. Afr J Biotechnol 9:6477–6481Google Scholar