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Journal of Plant Research

, Volume 126, Issue 6, pp 847–857 | Cite as

Phototropin 2 is involved in blue light-induced anthocyanin accumulation in Fragaria x ananassa fruits

  • Yasuko Kadomura-Ishikawa
  • Katsuyuki Miyawaki
  • Sumihare Noji
  • Akira TakahashiEmail author
Regular Paper

Abstract

Anthocyanins are widespread, essential secondary metabolites in higher plants during color development in certain flowers and fruits. In strawberries, anthocyanins are also key contributors to fruit antioxidant capacity and nutritional value. However, the effects of different light qualities on anthocyanin accumulation in strawberry (Fragaria x ananassa, cv. Sachinoka) fruits remain elusive. In the present study, we showed the most efficient increase in anthocyanin content occurred by blue light irradiation. Light sensing at the molecular level was investigated by isolation of two phototropin (FaPHOT1 and FaPHOT2), two cryptochrome (FaCRY1 and FaCRY2), and two phytochrome (FaPHYA and FaPHYB) homologs. Expression analysis revealed only FaPHOT2 transcripts markedly increased depending on fruit developmental stage, and a corresponding increase in anthocyanin content was detected. FaPHOT2 knockdown resulted in decreased anthocyanin content; however, overexpression increased anthocyanin content. These findings suggested blue light induced anthocyanin accumulation, and FaPHOT2 may play a role in sensing blue light, and mediating anthocyanin biosynthesis in strawberry fruits. This is the first report to find a relationship between visible light sensing, and color development in strawberry fruits.

Keywords

Anthocyanins Blue light Flavonoid pathway Photoreceptor Strawberry fruits 

Abbreviations

Cry

Cryptochrome

Phot

Phototropin

Phy

Phytochrome

LOV

Light, oxygen or voltage

PAL

Phenylalanine ammonia-lyase

CHS

Chalcone synthase

CHI

Chalcone isomerase

F3H

Flavanone 3-hydroxylase

DFR

Dihydroflavonol-4-reductase

ANS

Anthocyanidin synthase

FGT

Flavonoid glycosyltransferase

Notes

Acknowledgments

We thank Koichi Hayashi for donating strawberry fruit samples. We also thank Toshifumi Miki and Keisuke Hirota (Tokushima Agricultural Research Center, Japan) for invaluable advice and donations of strawberry fruit samples. Ourgenic Co., Ltd. supported this work (Tokushima, Japan). This work was also financially supported by the LED-Life project of the University of Tokushima, Japan.

Supplementary material

10265_2013_582_MOESM1_ESM.pptx (1.4 mb)
Supplementary material 1 (PPTX 1449 kb)
10265_2013_582_MOESM2_ESM.docx (110 kb)
Supplementary material 2 (DOCX 110 kb)

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Copyright information

© The Botanical Society of Japan and Springer Japan 2013

Authors and Affiliations

  • Yasuko Kadomura-Ishikawa
    • 1
  • Katsuyuki Miyawaki
    • 2
  • Sumihare Noji
    • 2
  • Akira Takahashi
    • 1
    Email author
  1. 1.Department of Nutrition, Faculty of MedicineThe University of TokushimaTokushimaJapan
  2. 2.Department of Life System, Institute of Technology and ScienceThe University of TokushimaTokushimaJapan

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