Transmission ratio distortion of molecular markers in a doubled haploid population originated from a natural hybrid between Osmunda japonica and O. lancea

Abstract

In ferns, intra-gametophytic selfing occurs as a mode of reproduction where two gametes from the same gametophyte form a completely homozygous sporophyte. Intra-gametophytic selfing is considered to be prevented by lethal or deleterious recessive genes in several diploid species. In order to investigate the modes and tempo of selection acting different developmental stages, doubled haploids obtained from intra-gametophytic selfing within isolated gametophytes of a putative F1 hybrid between Osmunda japonica and O. lancea were analyzed with EST_derived molecular markers, and the distribution pattern of transmission ratio distortion (TRD) along linkage map was clarified. As the results, the markers with skewness were clustered in two linkage groups. For the two highly distorted regions, gametophytes and F2 population were also examined. The markers skewed towards O. japonica on a linkage group (LG_2) showed skewness also in gametophytes, and the TRD was generated in the process of spore formation or growth of gametophytes. Also, selection appeared to be operating in the gametophytic stage. The markers on other linkage group (LG_11) showed highest skewness towards O. lancea in doubled haploids, and it was suggested that the segregation of LG_11 were influenced by zygotic lethality or genotypic evaluation and that some deleterious recessive genes exist in LG_11 and reduce the viability of homozygotes with O. japonica alleles. It is very likely that a region of LG_11were responsible for the low frequencies of intra-gametophytic selfing in O. japonica.

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Acknowledgments

We thank S. Matsumoto and W. Shinohara for assistance with the cultivation of materials. This study was supported by Grants-in-Aid number 20247006 to M.K. from the Japan Society for the Promotion of Science, research grant to Y.Y. from the Asahi Glass Foundation and that from the New Technology Development Foundation.

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Correspondence to Yoko Yatabe-Kakugawa.

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Y. Yatabe-Kakugawa is the recipient of the BSJ Award for Young Scientist, 2011.

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Yatabe-Kakugawa, Y., Tsutsumi, C., Hirayama, Y. et al. Transmission ratio distortion of molecular markers in a doubled haploid population originated from a natural hybrid between Osmunda japonica and O. lancea . J Plant Res 126, 469–482 (2013). https://doi.org/10.1007/s10265-012-0540-4

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Keywords

  • Intra-gametophytic selfing
  • Inbreeding depression
  • Doubled haploid
  • Osmunda
  • Transmission ratio distortion