Journal of Plant Research

, Volume 125, Issue 6, pp 805–807 | Cite as

Safer DNA extraction from plant tissues using sucrose buffer and glass fiber filter

Technical Note

Abstract

For some plant species, DNA extraction and downstream experiments are inhibited by various chemicals such as polysaccharides and polyphenols. This short communication proposed an organic-solvent free (except for ethanol) extraction method. This method consists of an initial washing step with STE buffer (0.25 M sucrose, 0.03 M Tris, 0.05 M EDTA), followed by DNA extraction using a piece of glass fiber filter. The advantages of this method are its safety and low cost. The purity of the DNA solution obtained using this method is not necessarily as high as that obtained using the STE/CTAB method, but it is sufficient for PCR experiments. These points were demonstrated empirically with two species, Japanese speedwell and common dandelion, for which DNA has proven difficult to amplify via PCR in past studies.

Keywords

DNA extraction Organic solvent free Polysaccharide Taraxacum Veronica 

Notes

Acknowledgments

This work was partly supported by a Grant-in-Aid for Young Scientists (B, No. 22710240 to K.-I.T.) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

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Copyright information

© The Botanical Society of Japan and Springer 2012

Authors and Affiliations

  1. 1.Osaka City Institute of Public Health and Environmental SciencesOsakaJapan

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