Safer DNA extraction from plant tissues using sucrose buffer and glass fiber filter
- 959 Downloads
For some plant species, DNA extraction and downstream experiments are inhibited by various chemicals such as polysaccharides and polyphenols. This short communication proposed an organic-solvent free (except for ethanol) extraction method. This method consists of an initial washing step with STE buffer (0.25 M sucrose, 0.03 M Tris, 0.05 M EDTA), followed by DNA extraction using a piece of glass fiber filter. The advantages of this method are its safety and low cost. The purity of the DNA solution obtained using this method is not necessarily as high as that obtained using the STE/CTAB method, but it is sufficient for PCR experiments. These points were demonstrated empirically with two species, Japanese speedwell and common dandelion, for which DNA has proven difficult to amplify via PCR in past studies.
KeywordsDNA extraction Organic solvent free Polysaccharide Taraxacum Veronica
This work was partly supported by a Grant-in-Aid for Young Scientists (B, No. 22710240 to K.-I.T.) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
- Kudo M, Kawabe K, Ookutsu S, Okamoto S, Yamakuchi H, Yasue H, Maeda Y, Shimogiri T (2010) Examination of the method of glass filter paper in the genomic DNA extraction of domestic animal/poultry. Bull Exp Farm Fac Agric Kagoshima Univ 32:13–17 (in Japanese)Google Scholar
- Takakura KI (2011) Improved method of plant DNA extraction using glass-fiber filter. Bunrui 11:139–149 (in Japanese)Google Scholar