Origin of Dryopteris shibipedis (Dryopteridaceae), a fern species extinct in the wild

Abstract

Dryopteris shibipedis was once treated as an extinct species in the Red List (2007) by the Japanese Ministry of the Environment, but ‘rediscovered’ in the Tsukuba Botanical Garden. To clarify its origin, using ‘overlooked’ cultivated stocks we analyzed nuclear PgiC intron sequences. As the PgiC genotype of D. shibipedis can be explained by a combination of alleles of D. kinkiensis and those of D. pacifica, the hypothesis of hybrid origin of the species is supported.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

References

  1. Ebihara A (2011) rbcL phylogeny of Japanese pteridophyte flora and implications on intrafamilial systematics. Bull Nat Mus Nat Sci B 37:63–74

    Google Scholar 

  2. Ebihara A, Ishikawa H, Matsumoto S, Lin S-J, Iwatsuki K, Takamiya M, Watano Y, Ito M (2005) Nuclear DNA, chloroplast DNA, and ploidy analysis clarified biological complexity of the Vandenboschia radicans complex (Hymenophyllaceae) in Japan and adjacent areas. Am J Bot 92:1535–1547

    PubMed  Article  CAS  Google Scholar 

  3. Ebihara A, Nitta JH, Ito M (2010) Molecular species identification with rich floristic sampling: DNA barcoding the pteridophyte flora of Japan. PLoS ONE 5:e15136

    PubMed  Article  Google Scholar 

  4. Fraser-Jenkins CR (1986) A classification of the genus Dryopteris (Pteridophyta: Dryopteridaceae). Bull Brit Mus Nat Hist (Bot) 14:183–218

    Google Scholar 

  5. Hirabayashi H (1974) Cytogeographic studies on Dryopteris of Japan. Hara Shobo, Tokyo

    Google Scholar 

  6. Ishikawa H, Watano Y, Kano K, Ito M, Kurita S (2002) Development of primer sets for PCR amplification of the PgiC gene in ferns. J Plant Res 115:65–70

    PubMed  Article  CAS  Google Scholar 

  7. Ito H (1938) Nova flora japonica, vol 4. Sanseido, Tokyo

    Google Scholar 

  8. Iwatsuki K (1992) Ferns and fern allies of Japan. Heibonsha, Tokyo (in Japanese)

    Google Scholar 

  9. Iwatsuki K, Yamazaki T, Boufford DE, Ohba H (1995) Flora of Japan, vol I. Kodansha, Tokyo

    Google Scholar 

  10. Japanese Ministry of Environment (2007) Red List of Japan, plant I (vascular plants). Japanese Ministry of Environment, Tokyo. http://www.env.go.jp/press/file_view.php?serial=9947&hou_id=8648

  11. Kurata S (1966) Notes on Japanese ferns (39). J Geobot 14:82–86

    Google Scholar 

  12. Kurata S (1978) Shida Sanka. Chikyusha, Tokyo (in Japanese)

    Google Scholar 

  13. Kurata S, Nakaike T (1979) Illustrations of Pteridophytes of Japan, vol 1. University of Tokyo Press, Tokyo

    Google Scholar 

  14. Kurata S, Nakaike T (1985) Illustrations of Pteridophytes of Japan, vol 4. University of Tokyo Press, Tokyo

    Google Scholar 

  15. Kurata S, Nakaike T (1997) Illustrations of Pteridophytes of Japan, vol 8. University of Tokyo Press, Tokyo

    Google Scholar 

  16. Lin S-J, Kato M, Iwatsuki K (1992) Diploid and triploid offspring of triploid agamosporous fern Dryopteris pacifica. J Plant Res 105:443–452

    Google Scholar 

  17. Lin S-J, Kato M, Iwatsuki K (2003) Morphological and cytological variations on Japanese Dryopteris varia group (Dryopteridaceae). In: Chandra S, Srivastava M (eds) Pteridology in the new millennium. Kluwer Academic Publishers, The Netherlands, pp 217–231

  18. Sheffield E (2008) Alternation of generations. In: Ranker TA, Haufler CH (eds) Biology and evolution of ferns and lycophytes. Cambridge University Press, New York., pp 49–74

    Google Scholar 

  19. Takamiya M (1996) Index to chromosomes of Japanese Pteridophyta (1910–1996). Japan Pteridological Society, Tokyo

    Google Scholar 

  20. Tanaka K (1999) Variation of Dryopteris pacifica in the western part of Kanagawa Prefecture. J Nippon Fern Club 3(19–20):1–7 (in Japanese)

    Google Scholar 

Download references

Acknowledgments

The authors thank K. Abe, Y. Hirayama, C. Tsutsumi, K. Uno and K. Yamada for their assistance of molecular analysis and spore cultivation, and also thank K. Kawahara and M. Otomasu for providing living materials.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Atsushi Ebihara.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ebihara, A., Matsumoto, S. & Kato, M. Origin of Dryopteris shibipedis (Dryopteridaceae), a fern species extinct in the wild. J Plant Res 125, 499–505 (2012). https://doi.org/10.1007/s10265-012-0474-x

Download citation

Keywords

  • Apogamy
  • Dryopteris
  • Extinct species
  • Hybrid
  • PgiC