Journal of Plant Research

, Volume 125, Issue 4, pp 499–505 | Cite as

Origin of Dryopteris shibipedis (Dryopteridaceae), a fern species extinct in the wild

  • Atsushi EbiharaEmail author
  • Sadamu Matsumoto
  • Masahiro Kato
Regular Paper


Dryopteris shibipedis was once treated as an extinct species in the Red List (2007) by the Japanese Ministry of the Environment, but ‘rediscovered’ in the Tsukuba Botanical Garden. To clarify its origin, using ‘overlooked’ cultivated stocks we analyzed nuclear PgiC intron sequences. As the PgiC genotype of D. shibipedis can be explained by a combination of alleles of D. kinkiensis and those of D. pacifica, the hypothesis of hybrid origin of the species is supported.


Apogamy Dryopteris Extinct species Hybrid PgiC 



The authors thank K. Abe, Y. Hirayama, C. Tsutsumi, K. Uno and K. Yamada for their assistance of molecular analysis and spore cultivation, and also thank K. Kawahara and M. Otomasu for providing living materials.


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Copyright information

© The Botanical Society of Japan and Springer 2012

Authors and Affiliations

  • Atsushi Ebihara
    • 1
    Email author
  • Sadamu Matsumoto
    • 1
  • Masahiro Kato
    • 1
  1. 1.Department of BotanyNational Museum of Nature and ScienceTsukubaJapan

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