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Sucrose transporter NtSUT4 from tobacco BY-2 involved in plant cell shape during miniprotoplast culture

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Abstract

Sucrose plays an important role in several cellular processes since it is a general source of metabolic energy, serves as a precursor for starch and cellulose synthesis, and is a metabolic starting point for carboxylate- and amino acid synthesis. While plant vacuole is the main cellular storage pool, where sucrose accumulates to high concentrations, only a small number of vacuolar sugar transporters have been identified and characterized to date. We initially identified a vacuolar sucrose transporter (NtSUT4) from tobacco BY-2 cells and established transgenic tobacco BY-2 cell lines that overexpress NtSUT4-GFP (BY-SUTG cells). Using a model system for synchronous cell elongation in miniprotoplasts (evacuolated cells) prepared from tobacco BY-2 cells, we found that NtSUT4-GFP overexpression inhibited cell growth towards the cell major axis. Moreover, under the same conditions, we found that the cell walls were well stained by calcofluor in BY-SUTG cells than in wild type BY-2 cells. These results suggest that NtSUT4 is involved in cell shape via sucrose homeostasis in plant cells.

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Abbreviations

BY-2:

Bright Yellow 2

BY-SUTG:

BY-2 cells expressing NtSUT4-GFP fusion proteins

FM4-64:

N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino)phenyl)hexatrienyl)-pyridinium dibromide

GFP:

Green fluorescent protein

Nt:

Nicotiana tabacum

SUT:

Sucrose transporter

VM:

Vacuolar membrane

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Acknowledgments

This study was financially supported in part by a Grant-in-Aid for Scientific Research on Priority Areas (Grant No. 19039008) from the Ministry of Education, Culture, Sports, Science and Technology, Japan and Institute for Bioinformatics Research and Development (BIRD) from Japan Science and Technology Agency to S.H. We are grateful to Dr. T. Nakagawa (Shimane University) for the kind gift of the pGWB451 binary vector.

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Authors and Affiliations

  1. Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha 5-1-5, Kashiwa, Chiba, 277-8562, Japan

    Emiko Okubo-Kurihara, Takumi Higaki, Natsumaro Kutsuna & Seiichiro Hasezawa

  2. Institute for Bioinformatics Research and Development (BIRD), Japan Science and Technology Agency (JST), Suita, Japan

    Natsumaro Kutsuna & Seiichiro Hasezawa

  3. Plant Genomic Network Research Team, Plant Functional Genomics Research Group, RIKEN Plant Science Center, Yokohama, Kanagawa, Japan

    Yukio Kurihara

  4. Faculty of Advanced Life Science and Graduate School of Life Science, Hokkaido University, Kita-ku N10-W8, Sapporo, 060-0810, Japan

    Junji Yamaguchi

Authors
  1. Emiko Okubo-Kurihara
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  2. Takumi Higaki
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  3. Yukio Kurihara
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  4. Natsumaro Kutsuna
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  5. Junji Yamaguchi
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  6. Seiichiro Hasezawa
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Corresponding author

Correspondence to Seiichiro Hasezawa.

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10265_2010_377_MOESM1_ESM.pptx

Fig. S1 Cell growth comparison between BY-2 cells and BY-SUTG cells. Fresh weights, dry weights and cell number were measured as described in Materials and Methods section. The experiments examined three independent measurements. Data represent mean values (n = 3, SEM). (PPTX 40 kb)

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Okubo-Kurihara, E., Higaki, T., Kurihara, Y. et al. Sucrose transporter NtSUT4 from tobacco BY-2 involved in plant cell shape during miniprotoplast culture. J Plant Res 124, 395–403 (2011). https://doi.org/10.1007/s10265-010-0377-7

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  • DOI: https://doi.org/10.1007/s10265-010-0377-7

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