Skip to main content

Advertisement

Log in

High-affinity nitrate uptake by rice (Oryza sativa) coleoptiles

  • Short Communication
  • Published:
Journal of Plant Research Aims and scope Submit manuscript

Abstract

Nitrate uptake by rice coleoptiles was evaluated using 15N-nitrate in relation to the expression of high-affinity nitrate uptake-related genes, OsNRT2s (OsNRT2.12.4) and OsNAR2s (OsNAR2.1 and 2.2). Apparent nitrate uptake by coleoptiles was about one-sixth of that by hydroponically cultured seedling roots. Real-time RT-PCR analysis revealed that OsNRT2.1, a root-specific key gene of inducible high-affinity transport system for nitrate, was most strongly induced in coleoptiles following nitrate supply initiation, while other OsNRT2s and OsNAR2s showed modest induction. These results suggest that rice coleoptiles may have high-affinity transport systems for nitrate similar to roots, and can be model organs for nutrient uptake by submerged plant shoots.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  • Alpi A, Beevers H (1983) Effects of O2 concentration on rice seedlings. Plant Physiol 71:30–34

    Article  CAS  PubMed  Google Scholar 

  • Araki R (2006) Structural genes consistent with high-affinity nitrate transport system in rice and reed. PhD thesis, University of Shiga Prefecture, Japan (in Japanese)

  • Araki R, Hasegawa H (2006) Expression of rice (Oryza sativa L.) genes involved in high-affinity nitrate transport during the period of nitrate induction. Breed Sci 56:295–302. doi:10.1270/jsbbs.56.295

    Article  CAS  Google Scholar 

  • Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction. Anal Biochem 162:156–159

    Article  CAS  PubMed  Google Scholar 

  • Esau K (1965) Plant anatomy, 2nd edn. Wiley, New York

    Google Scholar 

  • Gehrig HH, Winter K, Cushman J, Borland A, Taybi T (2000) An improved RNA isolation method for succulent plant species rich in polyphenols and polysaccharides. Plant Mol Biol Report 18:369–376. doi:10.1007/BF02825065

    Article  CAS  Google Scholar 

  • Hasegawa H, Ichii M (1994) Variation in Michaelis–Menten kinetic parameters for nitrate uptake by the young seedlings in rice (Oryza sativa L.). Breed Sci 44:383–386

    Google Scholar 

  • Hoagland RE, Paul RN (1978) A comparative SEM study of red rice and several commercial rice (Oryza sativa) varieties. Weed Sci 26:619–625

    Google Scholar 

  • Huang S, Greenway H, Colmer TD (2003) Responses by coleoptiles of intact rice seedlings to anoxia: K+ net uptake from the external solution and translocation from the caryopses. Ann Bot 91:271–278. doi:10.1093/aob/mcf122

    Article  CAS  PubMed  Google Scholar 

  • Magneschi L, Perata P (2009) Rice germination and seedling growth in the absence of oxygen. Ann Bot 103:181–196. doi:10.1093/aob/mcn121

    Article  CAS  PubMed  Google Scholar 

  • Okamoto M, Vidmar JJ, Glass ADM (2003) Regulation of NRT1 and NRT2 gene families of Arabidopsis thaliana: responses to nitrate provision. Plant Cell Physiol 44:304–317

    Article  CAS  PubMed  Google Scholar 

  • Orsel M, Krapp A, Daniel-Vedele F (2002) Analysis of the NRT2 nitrate transporter family in Arabidopsis. Structure and gene expression. Plant Physiol 129:886–896. doi:10.1104/pp.005280

    Article  CAS  PubMed  Google Scholar 

  • Reggiani R, Mattana M, Aurisano N, Bertani A (1993) Utilization of stored nitrate during the anaerobic germination of rice seeds. Plant Cell Physiol 34:379–383

    CAS  Google Scholar 

  • van Donk E, Gulati RD, Iedema A, Meulemans JT (1993) Macrophyte-related shifts in the nitrogen and phosphorus contents of the different trophic levels in a biomanipulated shallow lake. Hydrobiologia 251:19–26. doi:10.1007/BF00007160

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Hiroshi Hasegawa.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Takayanagi, S., Takagi, Y., Araki, R. et al. High-affinity nitrate uptake by rice (Oryza sativa) coleoptiles. J Plant Res 124, 305–309 (2011). https://doi.org/10.1007/s10265-010-0375-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10265-010-0375-9

Keywords

Navigation