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Paternal inheritance of mitochondria in Chlamydomonas

  • JPR Symposium
  • Cytoplasmic inheritance
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Abstract

To analyze mitochondrial DNA (mtDNA) inheritance, differences in mtDNA between Chlamydomonas reinhardtii and Chlamydomonas smithii, respiration deficiency and antibiotic resistance were used to distinguish mtDNA origins. The analyses indicated paternal inheritance. However, these experiments raised questions regarding whether paternal inheritance occurred normally. Mitochondrial nucleoids were observed in living zygotes from mating until 3 days after mating and then until progeny formation. However, selective disappearance of nucleoids was not observed. Subsequently, experimental serial backcrosses between the two strains demonstrated strict paternal inheritance. The fate of mt+ and mt− mtDNA was followed using the differences in mtDNA between the two strains. The slow elimination of mt+ mtDNA through zygote maturation in darkness was observed, and later the disappearance of mt+ mtDNA was observed at the beginning of meiosis. To explain the different fates of mtDNA, methylation status was investigated; however, no methylation was detected. Variously constructed diploid cells showed biparental inheritance. Thus, when the mating process occurs normally, paternal inheritance occurs. Mutations disrupting mtDNA inheritance have not yet been isolated. Mutations that disrupt maternal inheritance of chloroplast DNA (cpDNA) do not disrupt inheritance of mtDNA. The genes responsible for mtDNA inheritance are different from those of chloroplasts.

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Acknowledgments

This work was supported by a Grant-in-Aid for Scientific Research A to T.K. (No. 19207004) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. I am grateful to Dr. J.D. Reimer for a reading of the manuscript.

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  1. Laboratory of Cell and Functional Biology, Faculty of Science, University of the Ryukyus, Nishihara, Okinawa, 903-0123, Japan

    Soichi Nakamura

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  1. Soichi Nakamura
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Correspondence to Soichi Nakamura.

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Nakamura, S. Paternal inheritance of mitochondria in Chlamydomonas . J Plant Res 123, 163–170 (2010). https://doi.org/10.1007/s10265-009-0295-8

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  • DOI: https://doi.org/10.1007/s10265-009-0295-8

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