Rheophilous Osmunda lancea often hybridizes with a dryland ally, Osmunda japonica, to produce O. × intermedia, forming zonation in riverbanks and the adjacent dryland along flooding frequency clines. This study examined the genetic structure of populations consisting of O. × intermedia and the two parental species by analyzing ten nuclear DNA markers [six cleaved amplified polymorphic sequence (CAPS) markers and three simple sequence repeat (SSR) markers developed from an expressed sequence tag (EST) library, and the sequence of the glyceraldehyde-3-phosphate dehydrogenase gene GapCp] and chloroplast DNA sequences. The results suggest that the nuclear genes of O. japonica and O. lancea are genetically differentiated despite shared polymorphism in their chloroplast DNA sequences. This discrepancy may be attributable to natural selection and recent introgression, although it is not evident if introgression occurs between O. japonica and O. lancea in the examined populations. Our findings of putative F2 hybrids in O. × intermedia support its partial reproducibility, and also suggest that formation of later-generation hybrids generates morphological variation in O. × intermedia. O. lancea plants collected from geographically distant localities were genetically very similar, and it is suggested that O. lancea originated monotopically.
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Altshul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z (1997) Gapped Blast and PSI-BlAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402
Anderson EC, Thompson EA (2002) A model-based method for identifying species hybrids using multilocus genetic data. Genetics 160:1217–1229
Bowcock AM, Ruiz-Linares A, Tomfohrde J, Minch E, Kidd JR, Cavalli-Sforza LL (1994) High resolution of human evolutionary trees with polymorphic microsatellites. Nature 368:455–457
Falush D, Stephens M, Pritchard JK (2003) Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies. Genetics 164:1567–1587
Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
Funk DJ, Omland KE (2003) Species-level paraphyly and polyphyly: frequency, causes, and consequences, with insights from animal mitochondrial DNA. Annu Rev Ecol Evol Syst 34:397–423
Gottlieb LD (1972) Levels of confidence in analysis of hybridization in plants. Ann Mo Bot Gard 59:435–446
Hasebe M, Oumori T, Nakazawa M, Iwatsuki K, Kato M (1994) rbcL gene sequences gave new clue to evolutionary lineage for leptosporangiate ferns. Proc Natl Acad Sci USA 91:5730–5734
Heiser CB (1973) Introgression re-examined. Bot Rev 39:347–366
Hey J, Won YJ, Sivasundar A, Nielsen R, Markert JA (2004) Using nuclear haplotypes with microsatellites to study gene flow between recently separated Cichlid species. Mol Ecol 13:909–919
Imaichi R, Kato M (1992) Comparative leaf development of Osmunda lancea and O. japonica (Osmundaceae): heterochronic origin of rheophytic stenophylly. Bot Mag Tokyo 105:199–213
Kato M (2007) Distribution of Osmundaceae. Bull Natl Mus Nat Sci Ser B 33:81–90
Lexer C, Kremer A, Petit RJ (2006) Shared alleles in sympatric oaks: recurrent gene flow is a more parsimonious explanation than ancestral polymorphism. Mol Ecol 15:2007–2012
Martinsen GD, Whitham TG, Turek RJ, Keim P (2001) Hybrid populations selectively filter gene introgression between species. Evolution 55:1325–1335
Metzgar JS, Skog JE, Zimmer EA, Pryer KM (2008) The paraphyly of Osmunda is confirmed by phylogenetic analyses of seven plastid loci. Syst Bot 33:31–36
Muir G, Schlotterer C (2005) Evidence for shared ancestral polymorphism rather than recurrent gene flow at microsatellite loci differentiating two hybridizing oaks (Quercus spp.). Mol Ecol 14:549–561
Ohwi J (1957) Flora of Japan. Pteridophyta. Shibundo, Tokyo
Patterson N, Richter DJ, Gnerre S, Lander ES, Reich D (2006) Genetic evidence for complex speciation of humans and chimpanzees. Nature 441:1103–1108
Petersen J, Brinkmann H, Cerff R (2003) Origin, evolution, and metabolic role of a novel glycolytic GAPDH enzyme recruited by land plant plastids. J Mol Evol 57:16–26
Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959
Rieseberg LH, Wendel J (1993) Introgression and its consequences in plants. In: Harrison R (ed) Hybrid zones and the evolutionary process. Oxford University Press, Oxford, pp 70–114
Rieseberg LH, Whitton J, Linder CR (1996) Molecular marker incongruence in plant hybrid zones and phylogenetic trees. Acta Bot Neerl 45:243–262
Shimura Y (1964) Observations on the fertile fronds of Osmunda lancea var. latipinnula. J Jpn Bot 39:242–246
Shimura Y (1972) Study of reproduction of Osmunda × intermedia Sugimoto. J Geobot 20:38–42
Shimura Y, Matsumoto S (1977) On the chromosome association in meiosis of Osmunda × intermedia. J Jpn Bot 52:377–378
Swofford DL (2002) PAUP*. Phylogenetic Analysis Using Parsimony (*and other methods), version 4. Sinauer Associates, Sunderland
Taberlet P, Gielly L, Pautou G, Bouvet J (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol Biol 17:1105–1109
Tagawa M (1959) Colored illustrations of the Japanese Pteridophyta. Hoikusha, Osaka
Tsutsumi C, Kato M (2005) Molecular phylogenetic study on Davalliaceae. Fern Gaz 17:147–162
van Steennis CGGJ (1981) Rheophytes of the World. Sijthoff & Noordhoff, Alphen aan den Rijn
van Steennis CGGJ (1987) Rheophytes of the world: supplement. Allertonia 4:267–330
Yatabe Y, Nishida H, Murakami N (1999) Phylogeny of Osmundaceae inferred from rbcL nucleotide sequences and comparison to the fossil evidence. J Plant Res 112:397–404
Yatabe Y, Murakami N, Iwatsuki K (2005) Claytosmunda; a new subgenus of Osmunda (Osmundaceae). Acta Phytotax Geobot 56:127–128
We thank S. Akiyama, A. Ebihara, G. Kokubugata, S. Matsumoto and T. Minamitani for providing materials used in this study. We also thank N. Katayama and S. Koi for their collaborative field work and, M. Takamiya, M. Tanaka and S. Kobayashi for information on localities of Osmunda × intermedia and O. lancea. This study was supported by grants-in-aid numbers 1806295 (to Y.Y.) and 20247006 (to M.K.) from the Japan Society for the Promotion of Science.
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Yatabe, Y., Tsutsumi, C., Hirayama, Y. et al. Genetic population structure of Osmunda japonica, rheophilous Osmunda lancea and their hybrids. J Plant Res 122, 585 (2009). https://doi.org/10.1007/s10265-009-0254-4
- Expressed sequence tag
- Simple sequence repeat
- Cleaved amplified polymorphic sequence
- Genetic population structure