Abstract
The sphingoid long-chain base (LCB) composition of glucosylceramides was characterized in 31 species of Fabaceae including the model legumes Lotus japonicus and Medicago truncatula. With the exception of Lupinus texensis L, the 8-trans/cis-unsaturated isomers of 4-hydroxy-8-sphingenines [i.e., t18:1 (8t) plus t18:1 (8c)] were the major components in each species. In tribe Fabeae, each species from four genera—Pisum, Lathyrus, Lens, and Vicia—showed that more than 50% of dihydroxy sphingoid LCBs are 8-sphingenines [i.e., d18:1 (8t) plus d18:1 (8c)]. These results suggest that the sphingoid LCB composition of glucosylceramides reflects the phylogenetic relationships within the Fabeae.
This is a preview of subscription content, log in via an institution to check access.
References
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917
Doyle JJ (1997) Trees within trees: genes and species, molecules and morphology. Syst Biol 46:537–553
Imai H, Ohnishi M, Hotsubo K, Kojima M, Ito S (1997) Sphingoid base composition of cerebrosides from plant leaves. Biosci Biotechnol Biochem 61:351–353
Imai H, Morimoto Y, Tamura K (2000a) Sphingoid base composition of monoglucosylceramide in Brassicaceae. J Plant Physiol 157:453–456
Imai H, Yamamoto K, Shibahara A, Miyatani S, Nakayama T (2000b) Determining double-bond positions in monoenoic 2-hydroxy fatty acids of glucosylceramides by gas chromatography–mass spectrometry. Lipids 35:233–236
Ito S, Ohnishi M, Fujino Y (1985) Investigation of sphingolipids in pea seeds. Agric Biol Chem 49:539–540
Kojima M, Ohnishi M, Ito S (1991) Composition and molecular species of ceramide and cerebroside in scarlet runner beans (Phaseolus coccineus L.) and kidney beans (Phaseolus vulgaris L.). J Agric Food Chem 39:1709–1714
Lefebvre B, Furt F, Hartmann MA, Michaelson LV, Carde JP, Sargueil-Boiron F, Rossignol M, Napier JA, Cullimore J, Bessoule JJ, Mongrand S (2007) Characterization of lipid rafts from Medicago truncatula root plasma membranes: a proteomic study reveals the presence of a raft-associated redox system. Plant Physiol 144:402–418
Lewis G, Schrire B, Mackinder B, Lock M (eds) (2005) Legumes of the world. Kew, Richmond
Lock JM (2005) Trifolieae. In: Lewis G, Schrire B, Mackinder B, Lock M (eds) Legumes of the world. Kew, Richmond, pp 499–504
Markham JE, Li J, Cahoon EB, Jaworski JG (2006) Separation and identification of major plant sphingolipid classes from leaves. J Biol Chem 281:22684–22694
Ohnishi M, Fujino Y (1981) Chemical composition of ceramide and cerebroside in azuki bean seeds. Agric Biol Chem 45:1283–1284
Ohnishi M, Fujino Y (1982) Sphingolipids in immature and mature soybeans. Lipids 17:803–810
Ohnishi M, Ito S, Fujino Y (1982) Chemical characterization of ceramide and cerebroside in soybean leaves. Agric Biol Chem 46:2855–2856
Ohnishi M, Ito S, Fujino Y (1983) Characterization of sphingolipids in spinach leaves. Biochim Biophys Acta 752:416–422
Sperling P, Franke S, Lüthje S, Heinz E (2005) Are glucocerebrosides the predominant sphingolipids in plant plasma membranes? Plant Physiol Biochem 43:1031–1038
Acknowledgments
We wish to thank Dr. Kazuhiro Toyoda of Okayama University for his kind donation of the M. truncatula seeds. We also thank Chisako Taki, Maiko Asada, Chihiro Iwami, Naoko Shimada, and Kaoru Nishikawa for their contributions to our study.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Minamioka, H., Imai, H. Sphingoid long-chain base composition of glucosylceramides in Fabaceae: a phylogenetic interpretation of Fabeae. J Plant Res 122, 415–419 (2009). https://doi.org/10.1007/s10265-009-0227-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10265-009-0227-7