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Phylogeny of Chinese Polystichum (Dryopteridaceae) based on chloroplast DNA sequence data (trnL-F and rps4-trnS)

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Abstract

Polystichum is one of the largest and most taxonomically complex fern genera in China. The evolutionary relationships of Chinese Polystichum and related genera, and the relationship between our Polystichum phylogeny and ecogeographic distribution, were tested by the use of DNA sequence data. Fifty-one species of Polystichum and 21 species in allied genera were sequenced for the plastid intergenic spacers rps4-trnS and trnL-F. Maximum parsimony and Bayesian phylogenetic analyses of both individual and combined data sets showed that Chinese Polystichum as commonly recognized was paraphyletic: one clade (the CCPC clade) included Cyrtomidictyum lepidocaulon, two Cyrtogonellum species, three Cyrtomium species, and a small number of Polystichum species usually occurring on limestone. A second clade, Polystichum sensu stricto, included the remainder of the Polystichum species; these often occur on non-limestone substrates. The remaining Cyrtomium species formed the third clade. Three subclades resolved within Polystichum sensu stricto (s.s.) clade do not correspond with recent sectional classifications, and we outline the issues relevant to a new classification for the genus.

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Acknowledgments

This study was supported by a Major Research Project from the Chinese Academy of Science (KZCX2-SW-130), and the National Natural Science Foundation of China (40302003, 30370116).

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Authors and Affiliations

  1. State Key Laboratory of Palaeobiology and Stratigraphy of the Nanjing Institute of Geology and Palaeontology, The Chinese Academy of Science, Nanjing, Jiangsu Province, 210008, China

    Chun-xiang Li

  2. Institute of Ecology and Geobotany, Yunnan University, Kunming, Yunnan Province, China

    Shu-gang Lu

  3. Pringle Herbarium, Department of Plant Biology, The University of Vermont, Burlington, VT, USA

    David S. Barrington

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  1. Chun-xiang Li
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  2. Shu-gang Lu
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  3. David S. Barrington
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Correspondence to Chun-xiang Li.

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Li, Cx., Lu, Sg. & Barrington, D.S. Phylogeny of Chinese Polystichum (Dryopteridaceae) based on chloroplast DNA sequence data (trnL-F and rps4-trnS). J Plant Res 121, 19–26 (2008). https://doi.org/10.1007/s10265-007-0120-1

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