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Spatiotemporal metabolic regulation of anthocyanin and related compounds during the development of marginal picotee petals in Petunia hybrida (Solanaceae)


Structures and levels of anthocyanin-related compounds were analyzed during the development of marginal picotee petals in white-center and white-marginal cultivars of Petunia hybrida. In the white site of a white-center cultivar, higher concentrations of quercetin derivatives possessing 7-O-glucoside and/or 3′-O-glucoside occurred than in the colored site, suggesting that these two quercetin glycosylation steps are site-specifically regulated. The boundary areas of petal coloration were composed of cells showing various color densities, whose uniformity among adjacent cells varied between these cultivars. These results indicate diversity in spatiotemporal regulation of anthocyanin biosynthesis and flavonol glycosylations between Petunia cultivars during marginal picotee formation.

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  • Fukui Y, Kusumi T, Yoshida K, Kondo T, Matsuda C, Nomoto K (1998) Structures of two diacylated anthocyanins from Petunia hybrida cv. Surfinia Violet Mini. Phytochem 47:1409–1416

    Article  CAS  Google Scholar 

  • Griesbach RJ, Asen S (1990) Characterization of the flavonol glycosides in petunia. Plant Sci 70:49–56

    Article  CAS  Google Scholar 

  • Griesbach RJ, Asen S, Leonhardt BA (1991) Petunia hybrida anthocyanins acylated with caffeic acid. Phytochem 30:1729–1731

    Article  Google Scholar 

  • Hoshino A, Johzuka-Hisatomi Y, Iida S (2001) Gene duplication and mobile genetic elements in the morning glories. Gene 7:1–10

    Article  Google Scholar 

  • Inagaki Y, Hisatomi Y, Suzuki T, Kasahara K, Iida S (1994) Isolation of a Suppressor-mutator/Enhancer-like transposable element, Tpn1, from Japanese morning glory bearing variegated flowers. Plant Cell 6:375–383

    Article  PubMed  CAS  Google Scholar 

  • Itoh Y, Higeta D, Suzuki A, Yoshida H, Ozeki Y (2002) Excision of transposable elements from the chalcone isomerase and dihydroflavonol 4-reductase genes may contribute to the variegation of the yellow-flowered carnation (Dianthus caryophyllus). Plant Cell Physiol 43:578–585

    Article  PubMed  CAS  Google Scholar 

  • Koseki M, Goto K, Masuta C, Kanazawa A (2005) The star-type color pattern in Petunia hybrida “Red Star” flowers is induced by the sequence-specific degradation of the chalcone synthase RNA. Plant Cell Physiol 46:1879–1883

    Google Scholar 

  • Nielsen JK, Olsen CE, Petersen MK (1993) Acylated flavonol glycosides from cabbage leaves. Phytochem 34:539–544

    Article  CAS  Google Scholar 

  • Saito R, Fukuta N, Ohmiya A, Itoh Y, Ozeki Y, Kuchitsu K, Nakayama M (2006) Regulation of anthocyanin biosynthesis involved in the formation of marginalpicotee petals in Petunia. Plant Sci 170:828–834

    Article  CAS  Google Scholar 

  • Slimestad R, Aaberg A, Andersen ØM (1999) Acylated anthocyanins from petunia flowers. Phytochem 50:1081–1086

    Article  CAS  Google Scholar 

  • Tatsuzawa F, Toya Y, Watanabe H, Hirayama Y, Shinoda K, Hara R, Seki H, Ando T (2004) Acylated peonidin 3-rutinoside-5-glucosides from commercial petunia cultivars with pink flowers. Heterocycles 63:509–517

    Article  CAS  Google Scholar 

  • van Blokland R, Van der Geest N, Mol JNM, Kooter JM (1994) Transgene-mediated suppression of chalcone synthase expression in Petunia hybrida results from an increase in RNA turnover. Plant J 6:861–877

    Google Scholar 

  • Veit M, Geiger H, Czygan FC, Markham KR (1990) Malonylated flavone 5-O-glucosides in the barren sprouts of Equisetum arvense. Phytochem 29:2555–2560

    Article  CAS  Google Scholar 

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We thank Dr. Keiichi Tanaka (National Institute of Fruit Tree Science) for his kind assistance with NMR measurements. This work was supported by a Grant-in-Aid from the ministry of Agriculture, Forestry and Fisheries of Japan.

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Correspondence to Masayoshi Nakayama.

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Saito, R., Kuchitsu, K., Ozeki, Y. et al. Spatiotemporal metabolic regulation of anthocyanin and related compounds during the development of marginal picotee petals in Petunia hybrida (Solanaceae). J Plant Res 120, 563–568 (2007).

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