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Induction and inhibition of cyclobutane pyrimidine dimer photolyase in etiolated cucumber (Cucumis sativus) cotyledons after ultraviolet irradiation depends on wavelength

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Abstract

Under polychromatic ultraviolet (UV) irradiation (maximum energy at 327 nm) the activity of DNA photolyase specific to cyclobutane pyrimidine dimers (CPDs), CPD photolyase, increased by an amount which depended on UV irradiance, and the level of CPD photolyase gene (CsPHR) transcripts temporarily increased before the activity reached a constant level. UV light (>320 nm) was more effective than visible light at increasing CPD photolyase activity. In contrast, monochromatic UV irradiation at wavelengths <300 nm increased the level of CsPHR transcripts similarly to irradiation at wavelengths >320 nm, but reduced CPD photolyase activity compared with the dark control. Exposure of a CPD photolyase solution to UV-C (254 nm) reduced enzyme activity and induced accumulation of H2O2. Addition of H2O2 to the enzyme solution also inactivated CPD photolyase activity. These results suggest the possibility that reactive oxygen species participate in the inactivation of CPD photolyase in cotyledons exposed to UV irradiation of <300 nm.

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Abbreviations

CPD:

Cyclobutane pyrimidine dimer

CTAB:

Hexadecyltrimethylammonium bromide

DTT:

Dithiothreitol

ELISA:

Enzyme-linked immunosorbent assay

PBS:

Phosphate-buffered saline

ROS:

Reactive oxygen species

RT-PCR:

Reverse transcription-polymerase chain reaction

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Acknowledgments

Irradiation with monochromatic UV light was performed under the Cooperative Research Program of the National Institute for Basic Biology for the Okazaki large spectrograph. We thank Dr M. Watanabe and Mr S. Higashi of the Large Spectrograph Laboratory at the National Institute for Basic Biology for assistance with UV irradiation. This work was supported in part by Grants-in-Aid from the Japan Society for the Promotion of Science (Research for the Future Program, JSPS-RFTF96L00602) and from the Global Environment Research Fund of the Environment Agency of Japan.

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Author notes

  1. Megumi Hada

    Present address: Division of Space Life Sciences, NASA/Lyndon B. Johnson Space Center, 2102 NASA Parkway, Mail Code: SK, Houston, TX, 77058, USA

  2. Shinya Takahashi

    Present address: Plant Functional Genomics Research Team, Plant Functional Genomics Research Group, RIKEN Plant Science Center, RIKEN Yokohama Institute, Suehiro-cho 1-7-22, Tsurumi-ku, Yokohama, 230-0045, Japan

  3. Noriaki Kondo

    Present address: Department of Biosciences, Teikyo University of Science and Technology, Yatsusawa 2525, Uenohara, Yamanashi, 409-0193, Japan

Authors and Affiliations

  1. Department of Bioscience and Technology, School of Engineering, Hokkaido Tokai University, 5-1-1 Minami-sawa, Minami-ku, Sapporo, 005-8601, Japan

    Yuichi Takeuchi, Kazuya Takemura & Megumi Hada

  2. Course of Environmental and Biological Sciences, Graduated School of Science and Engineering, Hokkaido Tokai University, 5-1-1 Minami-sawa, Minami-ku, Sapporo, 005-8601, Japan

    Taku Inoue

  3. Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan

    Shinya Takahashi, Motohide Ioki & Noriaki Kondo

  4. Biodiversity Conservation Research Project, National Institute for Environmental Studies, Onogawa 16-2, Tsukuba, 305-8506, Japan

    Shinya Takahashi & Nobuyoshi Nakajima

Authors
  1. Yuichi Takeuchi
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  2. Taku Inoue
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Correspondence to Yuichi Takeuchi.

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Takeuchi, Y., Inoue, T., Takemura, K. et al. Induction and inhibition of cyclobutane pyrimidine dimer photolyase in etiolated cucumber (Cucumis sativus) cotyledons after ultraviolet irradiation depends on wavelength. J Plant Res 120, 365–374 (2007). https://doi.org/10.1007/s10265-006-0065-9

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  • DOI: https://doi.org/10.1007/s10265-006-0065-9

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