Abstract
Plant MADS-box genes encode transcriptional regulators that are critical for a number of developmental processes, such as the establishment of floral organ identity, flowering time, and fruit development. It appears that the MADS-box gene family has undergone considerable gene duplication and divergence within various angiosperm lineages. SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1)/Tomato MADS-box gene 3 (TM3)-like genes are members of the MADS-box gene family and have undergone repeated duplication events. Here, we isolated and characterized the SOC1/TM3-like gene TrcMADS1 from Trillium camtschatcense (Trilliaceae) to infer the ancestral function of SOC1/TM3-like genes. The alignment of SOC1/TM3-like genes revealed the presence of a highly conserved region in the C-terminal of predicted protein sequences, designated the SOC1 motif. Phylogenetic analysis indicated that TrcMADS1 is at the basal position of the SOC1/TM3-like gene family. The TrcMADS1 mRNA was detected in both vegetative and reproductive organs by RT-PCR. Our results suggest that duplicated copies of SOC1/TM3-like gene evolved to become variously functionally specialized.
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Acknowledgements
We thank Prof. M. Ohara for kindly providing plant materials. In addition, we thank Mr. H. Tokairin for collaboration in culturing the plants. We are additionally grateful to Y. Ishikawa, P.-Y. Yun, T. Ito, Y. Mashiko, H. Ashizawa, M. Nakada, R. Shinohara, M. Komatsu, S-Y. Kim, and M. Hirai for providing help and advice. This work was supported, in part, by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and by the Sasakawa Scientific Research Grant from the Japan Science Society.
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Nakamura, T., Song, IJ., Fukuda, T. et al. Characterization of TrcMADS1 gene of Trillium camtschatcense (Trilliaceae) reveals functional evolution of the SOC1/TM3-like gene family. J Plant Res 118, 229–234 (2005). https://doi.org/10.1007/s10265-005-0215-5
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DOI: https://doi.org/10.1007/s10265-005-0215-5