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Interaction between proliferating cell nuclear antigen (PCNA) and a DnaJ induced by DNA damage

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Abstract

Proliferating cell nuclear antigen (PCNA) is an essential protein for both DNA replication and DNA repair. In the present study using two-hybrid analysis with PCNA from rice, Oryza sativa L. cv. Nipponbare (OsPCNA), we found that OsPCNA interacted with rice DnaJ protein. We have identified DnaJ and designated it as OsDnaJ. OsDnaJ was able to bind to OsPCNA in vitro. Transcripts of OsDnaJ were found to be strongly expressed in the proliferating cells. mRNA of DnaJ was induced by UV and DNA-damaging agents such as H2O2. The expression patterns of OsPCNA were almost the same as OsDnaJ. The relationship between OsPCNA and OsDnaJ is discussed.

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Abbreviations

PCNA:

Proliferating cell nuclear antigen

OsPCNA:

Oryza sativa proliferating cell nuclear antigen

OsDnaJ:

Oryza sativa DnaJ

OsDnaK:

Oryza sativa DnaK

MMS:

Methyl methanesulfonate

HSP:

Heat shock protein

CPRG:

Chlorophenol red-β-D-galactopyranoside

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Acknowledgements

This work was supported in part by a grant from Futaba Electronics Memorial Foundation (Japan).

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Authors and Affiliations

  1. Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan

    Taichi Yamamoto, Yoko Mori, Toyotaka Ishibashi, Yukinobu Uchiyama, Tadamasa Ueda, Tsuyu Ando, Junji Hashimoto, Seisuke Kimura & Kengo Sakaguchi

  2. National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305-0854, Japan

    Tadamasa Ueda & Junji Hashimoto

  3. Institute of the Society for Techno-innovation of Agrobiological Sciences, Tsukuba, Ibaraki, 305-0854, Japan

    Tsuyu Ando

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  1. Taichi Yamamoto
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  2. Yoko Mori
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  3. Toyotaka Ishibashi
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  4. Yukinobu Uchiyama
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  9. Kengo Sakaguchi
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Correspondence to Kengo Sakaguchi.

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Yamamoto, T., Mori, Y., Ishibashi, T. et al. Interaction between proliferating cell nuclear antigen (PCNA) and a DnaJ induced by DNA damage. J Plant Res 118, 91–97 (2005). https://doi.org/10.1007/s10265-005-0197-3

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  • DOI: https://doi.org/10.1007/s10265-005-0197-3

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