Abstract
Using flow cytometry, we measured the nuclear DNA contents of all known taxa in Hepatica. Nuclear DNA content of Hepatica falconeri (diploid, crenate leaf lobes) was significantly lower than that of diploid entire species. Among the tetraploid species, crenate species had lower DNA contents than the entire taxon H. nobilis var. pubescens. The DNA content of the tetraploid species was more than double that of the diploid species among the same leaf-type groups.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bennett MD, Smith JB (1971) The 4C nuclear DNA content of several Hordeum genotypes. Can J Genet Cytol 13:607–611
Bennett MD, Smith JB (1976) Nuclear DNA amounts in angiosperms. Phil Trans R Soc Lond B 274:227–274
Bennetzen JL, Kellogg EA (1997) Do plants have a one-way ticket to genomic obesity? Plant Cell 9:1509–1514
Buitendijk JH, Boon EJ, Ramanna MS (1997) Nuclear content in twelve species of Alstroemeria L. and some of their hybrids. Ann Bot 79:343–353
Ehrendorfer F, Samuel R (2001) Contributions to a molecular phylogeny and systematics of Anemone and related genera (Ranunculaceae–Anemoninae). Acta Phytotax Sin 39:293–307
Furuta Y, Nishikawa K, Tanino T (1974) Stability in DNA content of AB genome component of common wheat during the past seven thousand years. Jpn J Genet 49:179–187
Hara H, Kurosawa S (1958) Differentiation within Anemone Hepatica L. of Japan. J Jpn Bot 33:265–274
Heimburger M (1959) Cytotaxonomic studies in the genus Anemone. Can J Bot 37:587–612
Hoot SB, Reznicek AA, Palmer JD (1994) Phylogenetic relationships in Anemone (Ranunculaceae) based on morphology and chloroplast DNA. Systematic Bot 19:169–200
Johnston JS, Bennett MD, Rayburn AL, Galbraith DW, Price HJ (1999) Reference standards for determination of DNA content of plant nuclei. Am J Bot 86:609–613
Kitamura S, Murata G (1962) New names and new conceptions adopted in our coloured illustration of herbaceous plants of Japan II (Chripetalae). Acta Phytotax Geobot 20:195–208
Kurita M (1955) Cytological studies in Ranunculaceae. II. The karyotype of Anemone and Hepatica. Bot Mag Tokyo 68:187–190
Kurita M (1957) Chromosome studies in Ranunculaceae. I. Karyotypes of the subtribe Anemoninae. Rep Biol Inst Ehime Univ 1:1–10
Mabuchi T (1998) Intra-species differentiation of Japanese Hepatica nobilis. PhD Thesis, Gifu University, Gifu, Japan
Mabuchi T, Ogisu M, Xu JM (1987) Chromosome number and karyotype of three species of Anemone hepatica group. J Jpn Bot 62:49–53
Manzini G, Narcellona ML, Avitabile M, Quadrifoglio F (1983) Interaction of diamidino-2-phenylindole (DAPI) with natural and synthetic nucleic acids. Nucleic Acids Res 11:8861–8876
Mishiba K, Ando T, Mii M, Watanabe H, Kokubun H, Hashimoto G, Marchesi E (2000) Nuclear DNA content as an index character discriminating taxa in the genus Petunia sensu Jussieu (Solanaceae). Ann Bot 85:665–673
Nishikawa K, Furuta Y (1969) DNA content per nucleus in relation to phylogeny of wheat and its relatives. Jpn J Genet 44:23–29
Ogisu M, Awan MR, Mabuchi T, Mikanagi Y (2002) Morphology, phenology and cytology of Hepatica falconeri in Pakistan. Kew Bull 57:943–953
Raina SN, Parida A, Koul KK, Salimath SS, Bisht MS, Raja V (1994) Associated chromosomal DNA changes in polyploids. Genome 37:560–564
Rees H, Walters MR (1965) Nuclear DNA and the evolution of wheat. Heredity 20:73–82
Rothfels K, Sexsmith E, Heimburger M, Kause MO (1966) Chromosome size and DNA content of species of Anemone L. and related genera (Ranunculaceae). Chromosoma (Berl) 20:54–74
Schuettpelz E, Hoot SB, Samuel R, Ehrendorfer F (2002) Multiple origins of southern hemisphere Anemone (Ranunculaceae) based on plasitid and nuclear sequence data. Plant Syst Evol 231:143–151
Soltis DE, Soltis PS, Bennett MD, Leitch IJ (2003) Evolution of genome size in the angiosperms. Am J Bot 90:1596–1603
Thomson T (1852) Anemone falconeri Thoms. Hooker’s Icones Plantarum 9:t899
Acknowledgements
The authors would like to thank Kei-ichiro Mishiba of Iwate Bio-technology Research Center for the useful information; Kozo Nishikawa and Yoshihiko Furuta of Gifu University, and Katsumi Kataoka of Tamagawa University for their help with the statistical analysis. We also thank the following people for supplying the materials: Mikinori Ogisu (Hepatica henryi and H. yamatutai), Koichi Iwafuchi (H. nobilis from Spain), Tokio Naito (H. transsilvanica), and Kyohei Matsuda (H. nobilis from Switzerland).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Mabuchi, T., Kokubun, H., Mii, M. et al. Nuclear DNA content in the genus Hepatica (Ranunculaceae). J Plant Res 118, 37–41 (2005). https://doi.org/10.1007/s10265-005-0191-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10265-005-0191-9