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VEGF-A/HGF induce Prox-1 expression in the chick embryo chorioallantoic membrane lymphatic vasculature

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Abstract

Vascular endothelial growth factor-A (VEGF-A) and hepatocyte growth factor (HGF) are well known angiogenesis inductors and promoters in normal and pathologic conditions. Recent data showed that VEGF-A and HGF could also influence lymphangiogenesis but this matter has not been completely elucidated. Administration of VEGF-A and HGF in combination has been used to improve the angiogenic response in different experimental models, but their effects on lymphangiogenesis have not been investigated. The aim of this study was to characterize blood and lymphatic vascular response to VEGF-A/HGF administration. To this purpose, we built a pBlast VEGF-A/HGF combination suitable for in vivo research. By using as an experimental in vivo model the chick embryo chorioallantoic membrane (CAM) assay, we applied pBlast VEGF-A/HGF combination for 7 days. Results showed that VEGF-A/HGF combination was able to induce a strong angiogenic response and the expression of Prox-1 in the lymphatic endothelial cells of the CAM. The possible mechanisms involved have been speculated.

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Acknowledgments

This work was supported by grants PN II 41-054, IDEI 1147/2009 of Romanian Ministry of Research and Education, grant MIUR (PRIN 2007) and grant 41-052/2007 of Romanian Ministry of Education and Research.

Conflict of interest statement

The authors declare that they have no conflict of interest related to the publication of this manuscript.

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Authors and Affiliations

  1. Department of Histology, “Victor Babes” University of Medicine and Pharmacy, Timisoara, Romania

    Anca Maria Cimpean, Raluca Ceauşu, Pusa Gaje, Stefana Feflea & Marius Raica

  2. Department of Biochemistry, “Victor Babes” University of Medicine and Pharmacy, Timisoara, Romania

    Edward Seclaman & Andrei Anghel

  3. Department of Human Anatomy and Histology, University of Bari Medical School, Piazza G. Cesare, 11, 70124, Bari, Italy

    Domenico Ribatti

Authors
  1. Anca Maria Cimpean
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  2. Edward Seclaman
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  3. Raluca Ceauşu
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  4. Pusa Gaje
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  5. Stefana Feflea
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  6. Andrei Anghel
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  7. Marius Raica
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  8. Domenico Ribatti
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Correspondence to Domenico Ribatti.

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Cimpean, A.M., Seclaman, E., Ceauşu, R. et al. VEGF-A/HGF induce Prox-1 expression in the chick embryo chorioallantoic membrane lymphatic vasculature. Clin Exp Med 10, 169–172 (2010). https://doi.org/10.1007/s10238-009-0085-6

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  • DOI: https://doi.org/10.1007/s10238-009-0085-6

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