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In vitro and in vivo study of human amniotic fluid-derived stem cell differentiation into myogenic lineage

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Abstract

Recent findings have shown that amniotic fluid (AF) could be a putative new source of multipotent stem cells (SC). We investigated whether these human SC could efficiently differentiate into myogenic lineage in vitro and integrate in vivo skeletal muscle in severe combined immunodeficiency (SCID) mice. C/kit immunomagnetic-sorted AF (AF c/kit+) SC were characterized by immunocytochemistry and Southern blotting for myogenic markers (desmin, MyoD). In vitro, AF c/kit+ SC phenotypic conversion into myogenic cells was assayed by myogenic-specific induction media. AF c/kit+ SC without ex vivo manipulation were transplanted into the tibialis anterior (TA) of (SCID) mice. Acquisition of a myogenic-like phenotype (desmin, MyoD) in AF c/kit+ SC was observed after culture in myogenic-specific induction media. In vivo, transplanted AF c/kit+ SC showed an engraftment in the skeletal muscle of SCID mice, but with unexpected tubular glandular tissue-like differentiation. Importantly, no immuno-rejection, inflammatory response or tumorigenicity of these cells was found. Within these experimental conditions, AF c/kit+ SC were able to differentiate into myogenic cells in vitro, but not in vivo after their transplantation into the skeletal muscle of SCID mice. Because AF c/kit+ SC survived and differentiated into tubular gland-like cells after their transplantation in the TA, an ex vivo engagement in myogenic pathway prior their transplantation could favor their differentiation into myogenic cells in vivo.

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Acknowledgments

We are grateful to J. Puymirat, MD, PhD, for his support in this ongoing multidisciplinary project. We also thank J. Leroy for his excellent technical assistance. G. Walther was supported with a post-doctoral grant from Quebec Foundation for Health Research. O.F. Bertrand is a research scholar from Quebec Foundation for Health Research.

Conflict of interest statement

The authors declare that they have no conflict of interest related to the publication of this manuscript.

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Authors and Affiliations

  1. Laboratoire de Génétique Humaine, Centre Hospitalier de l’Université Laval, Quebec City, QC, Canada

    Jean Gekas, Guillaume Walther, Daniel Skuk & Emmanuel Bujold

  2. Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, 2725, chemin Ste Foy, Quebec City, QC, G1V 4G5, Canada

    Guillaume Walther & Olivier François Bertrand

  3. Laboratoire d’Anatomo-pathologie, CHUQ (Hôtel-Dieu), Quebec City, QC, Canada

    Isabelle Harvey

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  1. Jean Gekas
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  2. Guillaume Walther
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Correspondence to Olivier François Bertrand.

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Gekas, J., Walther, G., Skuk, D. et al. In vitro and in vivo study of human amniotic fluid-derived stem cell differentiation into myogenic lineage. Clin Exp Med 10, 1–6 (2010). https://doi.org/10.1007/s10238-009-0060-2

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  • DOI: https://doi.org/10.1007/s10238-009-0060-2

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