Immunological alterations in lupus-prone autoimmune (NZB/NZW) F1 mice by mycelia Chinese medicinal fungus Cordyceps sinensis-induced redistributions of peripheral mononuclear T lymphocytes

  • Jiun-Liang Chen
  • Yen-Chen Chen
  • Sien-Hung Yang
  • Yun-Fei Ko
  • Shao-Yuan ChenEmail author
Original Article


Mycelia products from wild-form Cordyceps sinensis could be constantly produced in a large scale and would be a better source of this herbal medicine. Our purpose was to investigate the immunological effects of an orally administered hot-water extract cultured mycelium of C. sinensis in lupus-prone (NZB/NZW) F1 hybrids. Forty female mice were divided into four groups and were given 2.4 mg/g/day oral doses of C. sinensis starting at three (group A), six (group B), or eight (group C) months of age, whereas the remaining group (group D) served as a control. Survival, proteinuria, and titers of anti-double-stranded DNA autoantibodies were evaluated. Treatment with C. sinensis resulted in increased survival, decreased proteinuria, and reduced titers of anti-double-stranded DNA antibody in groups A and B. Moreover, the mice in groups A and B showed significantly reduced percentages of CD4+ T cells (*P < 0.05) and increased percentages of CD8+ T cells in peripheral blood mononuclear cells (PBMC) after C. sinensis administration. At 6 months of age, the proliferation rate of BrdU-incorporated spleen cells was significantly decreased after 48 and 72 h of C. sinensis treatment (**P < 0.01) in group A of mice. In conclusions, early medication with C. sinensis induced the redistribution of PBMC and attenuated the disease severity of lupus in (NZB/NZW) F1 mice.


Lupus Cordyceps sinensis (NZB/NZW) F1 mice Immune modulation 



This research was supported in part by grants NSC 92-2320-B-016-051 from National Science Council, TSGH-C93-28 from Tri-Service General Hospital Research, and C Y Foundation for Advancement of Education, Sciences and Medicine.

Conflict of interest statement

The authors declare that they have no conflict of interest related to the publication of this manuscript.


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Jiun-Liang Chen
    • 1
  • Yen-Chen Chen
    • 2
  • Sien-Hung Yang
    • 1
  • Yun-Fei Ko
    • 3
  • Shao-Yuan Chen
    • 2
    • 4
    Email author
  1. 1.Graduate Institute and School of Traditional Chinese MedicineChang-Gung UniversityTaoyuanTaiwan, ROC
  2. 2.Graduate Institute of Medical SciencesNational Defense Medical CenterTaipeiTaiwan, ROC
  3. 3.Department of Biochemical EngineeringMingchi University of TechnologyTaipeiTaiwan, ROC
  4. 4.Department of Hyperbaric MedicineCardinal Tien HospitalHsintienTaiwan, ROC

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